Pricing Availability   Qty
Description: Green-yellow lucifer yellow-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name:R)-α,6-Diamino-1,3-dioxo-5,8-disulfo-1H-benz[de]isoquinoline-2(3H)-propanoic acid
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for YADA

YADA is a green-yellow lucifer yellow-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Exhibits large stokes shift and wider emission spectra than other FDAAs, allowing excitation via a violet light source and detection with a green filter. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~426/535 nm.

Optical Data for YADA

YADA Dye Spectra
Emission Color Green-yellow
λabs 426 nm
λem 535 nm
Extinction Coefficient (ε) 24200 M-1cm-1
Quantum Yield (φ) 0.2
Application Confocal Microscopy, Super Resolution Microscopy

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Technical Data for YADA

M. Wt 459.41
Formula C15H13N3O10S2
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 1471982-33-8
PubChem ID 134159307
Smiles O=C(N(C[C@@H](N)C(O)=O)C1=O)C2=C(C1=CC(S(=O)(O)=O)=C3N)C3=CC(S(=O)(O)=O)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for YADA

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 45.94 100

Preparing Stock Solutions for YADA

The following data is based on the product molecular weight 459.41. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.18 mL 10.88 mL 21.77 mL
5 mM 0.44 mL 2.18 mL 4.35 mL
10 mM 0.22 mL 1.09 mL 2.18 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

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References for YADA

References are publications that support the biological activity of the product.

Hsu et al (2017) Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 8 6313 PMID: 28989665

If you know of a relevant reference for YADA, please let us know.

Keywords: YADA, YADA supplier, Fluorescent, D-amino, acid, FDAA, labeling, peptidoglycans, bacteria, Probes, for, Imaging, Bacteria, Unnatural, Amino, Acids, 6650, Tocris Bioscience

Citations for YADA

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals