YADA

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Cat.No. 6650 - YADA | C15H13N3O10S2 | CAS No. 1471982-33-8
Description: Green-yellow lucifer yellow-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name:R)-α,6-Diamino-1,3-dioxo-5,8-disulfo-1H-benz[de]isoquinoline-2(3H)-propanoic acid
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity

Green-yellow lucifer yellow-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Exhibits large stokes shift and wider emission spectra than other FDAAs, allowing excitation via a violet light source and detection with a green filter. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~426/535 nm.

Optical Data for YADA

YADA Dye Spectra
Emission Color Yellow
λabs 426 nm
λem 535 nm
Extinction Coefficient (ε) 24200 M-1cm-1
Quantum Yield (φ) 0.2

Technical Data

M. Wt 459.41
Formula C15H13N3O10S2
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 1471982-33-8
PubChem ID 134159307
InChI Key YXWYHJBYHLJEIR-UHFFFAOYSA-N
Smiles O=C(N(C[C@@H](N)C(O)=O)C1=O)C2=C(C1=CC(S(=O)(O)=O)=C3N)C3=CC(S(=O)(O)=O)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 45.94 100

Preparing Stock Solutions

The following data is based on the product molecular weight 459.41. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.18 mL 10.88 mL 21.77 mL
5 mM 0.44 mL 2.18 mL 4.35 mL
10 mM 0.22 mL 1.09 mL 2.18 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

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References

References are publications that support the biological activity of the product.

Hsu et al (2017) Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 8 6313 PMID: 28989665


If you know of a relevant reference for YADA, please let us know.

Keywords: YADA, YADA supplier, Fluorescent, D-amino, acid, FDAA, labeling, peptidoglycans, bacteria, Probes, for, Imaging, Bacteria, Unnatural, Amino, Acids, 6650, Tocris Bioscience

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