TRAM 34

Pricing Availability Delivery Time Qty
Cat.No. 2946 - TRAM 34 | C22H17ClN2 | CAS No. 289905-88-0
Description: Potent and highly selective KCa3.1 channel blocker
Chemical Name: 1-[(2-Chlorophenyl)diphenylmethyl]-1H-pyrazole
Purity: ≥99% (HPLC)
Datasheet
Citations (8)
Literature

Biological Activity

Potent and highly selective blocker of intermediate conductance Ca2+-activated K+ channels (KCa3.1) (Kd = 20 nM). Exhibits 200-1500-fold selectivity over KV, BKCa, KCa2, Na+, CRAC and Cl- channels. Suppresses the reactivation of lymphocytes by mitogenic stimuli.

Technical Data

M. Wt 344.84
Formula C22H17ClN2
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 289905-88-0
PubChem ID 656734
InChI Key KBFUQFVFYYBHBT-UHFFFAOYSA-N
Smiles ClC(C=CC=C4)=C4C(N2C=CC=N2)(C3=CC=CC=C3)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 3.45 10mM with gentle warming
ethanol 1.72 5mM with sonication

Preparing Stock Solutions

The following data is based on the product molecular weight 344.84. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.9 mL 14.5 mL 29 mL
5 mM 0.58 mL 2.9 mL 5.8 mL
10 mM 0.29 mL 1.45 mL 2.9 mL
50 mM 0.06 mL 0.29 mL 0.58 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References

References are publications that support the products' biological activity.

Schilling and Eder (2007) TRAM-34 inhibits nonselective cation channels. Pflugers Arch. 454 559 PMID: 17318643

Wang et al (2007) An intermediate-conductance Ca2+-activated K+ channel mediates B lymphoma cell cycle progression induced by serum. Pflugers Arch. 454 945 PMID: 17429684

Wulff et al (2000) Design of a potent and selective inhibitor of the intermediate-conductance Ca2+-activated K+ channel, IKCa1: a potential immunosuppressant. Proc.Natl.Acad.Sci. USA 97 8151 PMID:


If you know of a relevant reference for TRAM 34, please let us know.

View Related Products by Product Action

View all Calcium-Activated Potassium (KCa) Channel Blockers

Keywords: selective IKCa1 channel blockers Potassium KCa Channels ca2+-activated ca2+-dependent K+ TRAM34 KCa3.1 Ca2+-Activated Potassium Channels

8 Citations for TRAM 34

Citations are publications that use Tocris products. Selected citations for TRAM 34 include:

Engbers et al (2012) Intermediate conductance calcium-activated potassium channels modulate summation of parallel fiber input in cerebellar Purkinje cells. Elife 109 2601 PMID: 22308379

Zemkova et al (2011) Norepinephrine causes a biphasic change in mammalian pinealocye membrane potential: role of alpha1B-adrenoreceptors, phospholipase C, and Ca2+. Endocrinology 152 3842 PMID: 21828176

Chan and Cipolla (2011) Relaxin causes selective outward remodeling of brain parenchymal arterioles via activation of peroxisome proliferator-activated receptor-γ. JCI Insight 25 3229 PMID: 21602449

Mazzuca et al (2015) Estrogen receptor subtypes mediate distinct microvascular dilation and reduction in [Ca2+]I in mesenteric microvessels of female rat. J Pharmacol Exp Ther 352 291 PMID: 25472954

Cahalan et al (2015) Piezo1 links mechanical forces to red blood cell volume. J Biomed Sci 4 PMID: 26001274

Chien and Su (2015) 5-hydroxytryptamine has an endothelium-derived hyperpolarizing factor-like effect on coronary flow in isolated rat hearts. PLoS One 22 42 PMID: 26076928

Benton et al (2013) Iberiotoxin-sensitive and -insensitive BK currents in Purkinje neuron somata. J Neurophysiol 109 2528 PMID: 23446695

Agarwal et al (2013) TRAM-34, a putatively selective blocker of intermediate-conductance, calcium-activated potassium channels, inhibits cytochrome P450 activity. FASEB J 8 e63028 PMID: 23667566


Do you know of a great paper that uses TRAM 34 from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for TRAM 34

Protocols

TODO: Add Protocols