Tissue Clearing Pro-Organoid

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Description: 3D cell culture clearing reagent kit
Literature (1)

Biological Activity for Tissue Clearing Pro-Organoid

Tissue Clearing Pro-Organoid is a rapid, easy-to-use, reversible and non-destructive kit, which allows effective tissue clearing and staining of organoids, 3D cell cultures and microtissues. Tissue Clearing Pro-Organoid is a solvent based tissue clearing technique, comparable to BABB and DISCO. Tissue Clearing Pro-Organoid does not cause significant shrinkage or tissue damage and does not use as harsh a solvent as other solvent based techniques.

This organoid tissue clearing kit allows 3D cell cultures up to 500 μm thick to be cleared in minutes, and is compatible with fluorescent protein, immunolabeling and small molecule staining techniques, as well as being suitable for use with all standard dyes and buffers. Following 3D imaging, Tissue Clearing Pro-Organoid can be reversed for follow-up 2D histology staining. The kit comes with a getting started guide and a more comprehensive tissue clearing guidebook, providing detailed protocols. Tissue Clearing Pro-Organoid kit contains 30 mL of Tissue Clearing Pro-Organoid clearing reagent plus the 4 buffer solutions required to process 3D cell cultures for immunolabeling and is enough to process three 96-well plates of material.

Please read the Tissue Clearing Pro-Organoid Guidebook for more information and detailed protocols for using this kit.

Scientific Data

Tissue Clearing Pro-Organoid Applications. Tissue Clearing Pro-Organoid Kit (Catalog # 7390) was applied to open liver spheroids, allowing visualization of stained structures: Left DNA, MRPII and MDRI; Middle DNA, CD68, Albumin and Vimentin; Right DNA, panCK and CD31. A wide range of IHC validated antibodies are available from R&D Systems and Novus Biologicals; optimization of antibody incubation conditions for use with the Tissue Clearing Pro-Organoid technique is recommended.

Technical Data for Tissue Clearing Pro-Organoid

Storage Store at RT

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Tissue Clearing Pro-Organoid

Certificate of Analysis / Product Datasheet
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References for Tissue Clearing Pro-Organoid

References are publications that support the biological activity of the product.

Buono et al (2020) Human cardiac organoids for modeling genetic cardiomyopathy. Cells 9 1733 PMID: 32698471

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals