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Tissue Clearing Pro-Organoid is a rapid, easy-to-use, reversible and non-destructive kit, which allows effective tissue clearing and staining of organoids, 3D cell cultures and microtissues. This organoid tissue clearing kit allows 3D cell cultures up to 500 μm thick to be cleared in minutes, and is compatible with fluorescent protein, immunolabeling and small molecule staining techniques, as well as being suitable for use with all standard dyes and buffers. Following 3D imaging, Tissue Clearing Pro-Organoid can be reversed for follow-up 2D histology staining. The kit comes with a getting started guide and a more comprehensive tissue clearing guidebook, providing detailed protocols. Tissue Clearing Pro-Organoid kit contains 30 mL of Tissue Clearing Pro-Organoid clearing reagent plus the 4 buffer solutions required to process 3D cell cultures for immunolabeling and is enough to process three 96-well plates of material.
A wide range of IHC validated antibodies are available from R&D Systems and Novus Biologicals; optimization of antibody incubation conditions for use with the Tissue Clearing Pro-Organoid technique is recommended.
Please read the Tissue Clearing Pro-Organoid guidebook for more information and detailed protocols for using this kit.
Guidebook and Protocol for Use
For information on how to use Tissue Clearing Pro-Organoid download our Guidebook.
Tissue Clearing Pro-Organoid Applications
Tissue Clearing Pro-Organoid Kit (Cat. No 7390) was applied to Open Liver HepaRG NP spheroids, allowing visualization of stained structures: Left DNA, MRPII and MDRI; Middle DNA, CD68, Albumin and Vimentin; Right DNA, panCK and CD31.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
References are publications that support the biological activity of the product.
Buono et al (2020) Human cardiac organoids for modeling genetic cardiomyopathy. Cells 9 1733 PMID: 32698471
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