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Submit ReviewTFAX 594, SE
Description: Red fluorescent dye; amine reactive
Chemical Name: 6-[2-Carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-1,2,10,11-tetrahydro-1,2,2,10,10,11-hexamethyl-4,8-bis(sulfomethyl)pyrano[3,2-g:5,6-g']diquinolin-13-ium inner salt
Biological Activity
Amine reactive red fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, 2-photon excitation microscopy, and super resolution microscopy techniques, such as dSTORM. Excitation/emission maxima ~ 590 / 660 nm; extinction coefficient = 92,000 M-1cm-1; quantum yield = 0.66.
Optical Data for TFAX 594, SE
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 590 nm |
| λem | 617 nm |
| Extinction Coefficient (ε) | 92,000 M-1cm-1 |
| Quantum Yield (φ) | 0.66 |
| Closest Laser line | 594 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Alternative For | Alexa Fluor® 594, Texas Red®, Bodipy-TR |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
| M. Wt | 819.85 |
| Formula | C39H37N3O13S2 |
| Storage | Store at -20°C |
| CAS Number | 295348-87-7 |
| PubChem ID | 25137945 |
| InChI Key | KWHCBZDWVYWWLE-UHFFFAOYSA-N |
| Smiles | CC(C=C1CS(=O)(O)=O)(C)N(C)C2=C1C=C(C(C3=CC=C(C(ON4C(CCC4=O)=O)=O)C=C3C(O)=O)=C5C(O6)=CC7=[N+](C)C(C)(C)C=C(CS(=O)([O-])=O)C7=C5)C6=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solubility | Soluble in DMSO |
Product Datasheets
References
References are publications that support the biological activity of the product.
Panchuk–Voloshina et al (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J.Histochem.Cytochem. 47 1179 PMID: 10449539
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