SPRi 3

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Description: Potent sepiapterin reductase (SPR) inhibitor; attenuates proliferation of CD4+ T cells
Chemical Name: N-[2-(5-Hydroxy-2-methyl-1H-indol-3-yl)ethyl]-2-methoxyacetamide
Purity: ≥98% (HPLC)

Biological Activity for SPRi 3

SPRi 3 is a potent sepiapterin reductase (SPR) inhibitor (IC50 = 53 - 74 nM for human SPR). Inhibits BH4 synthesis pathway and attenuates proliferation in naive CD4+ T cells. Suppresses proliferation of human effector CD4+ T cells following stimulation. Reduces SPR activity in mouse primary cultures of sensory neurons (IC50 = 450 nM). Ameliorates colitis, suppressing the intestinal infiltration of T cells and other immune cells in vivo. Also reduces immune-cell infiltration into mouse lungs. Reduces neuropathic and inflammatory pain in mouse models.

Licensing Information

Sold under license from the Children's Medical Center Corporation.

Compound Libraries for SPRi 3

SPRi 3 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for SPRi 3

M. Wt 262.3
Formula C14H18N2O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1292285-54-1
PubChem ID 52911386
Smiles COCC(NCCC1=C(NC2=CC=C(C=C12)O)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for SPRi 3

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 26.23 100
ethanol 26.23 100

Preparing Stock Solutions for SPRi 3

The following data is based on the product molecular weight 262.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.81 mL 19.06 mL 38.12 mL
5 mM 0.76 mL 3.81 mL 7.62 mL
10 mM 0.38 mL 1.91 mL 3.81 mL
50 mM 0.08 mL 0.38 mL 0.76 mL

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Product Datasheets for SPRi 3

Certificate of Analysis / Product Datasheet
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References for SPRi 3

References are publications that support the biological activity of the product.

Haruki et al (2016) Tetrahydrobiopterin biosynthesis as a potential target of the kynurenine pathway metabolite xanthurenic acid. J.Biol.Chem. 291 652 PMID: 26565027

Latremoliere et al (2015) Reduction of neuropathic and inflammatory pain through inhibition of the tetrahydrobiopterin pathway. Neuron 86 1393 PMID: 26087165

Cronin et al (2018) The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature 563 564 PMID: 30405245

If you know of a relevant reference for SPRi 3, please let us know.

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Keywords: SPRi 3, SPRi 3 supplier, SPRi3, sepiapterin, reductase, SPR, inhibitors, inhibits, tetrahydrobiopterin, pathway, BH4, Tcells, T, cells, CD4+, Other, Reductase, 6597, Tocris Bioscience

Citations for SPRi 3

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