Pricing Availability   Qty
Description: FDAA for super-resolution microscopy of bacteria
Chemical Name: 2-((1E,3E)-5-((E)-1-(6-(((R)-6-Amino-5-ammonio-6-oxohexyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)penta-1,3-dien-1-yl)-1,3,3-trimethyl-3H-indol-1-ium-5-sulfonate
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for sCy5DL-amide

sCy5DL-amide is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into Gram-positive B.subtilis. sCy5DL-amide labeled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/666 nm.

Scientific Data

Fluorescence Imaging Bacillus subtilis cells stained with sCy5DA-amide View Larger

Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA-amide

Optical Data for sCy5DL-amide

sCy5DL-amide Dye Spectra
Emission Color Red
λabs 646 nm
λem 666 nm
Extinction Coefficient (ε) 209,200 M-1cm-1
Cell Permeable Yes
Application STORM

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for sCy5DL-amide

M. Wt 769.97
Formula C38H51N5O8S2
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles CC1(C2=C(N(/C1=C/C=C/C=C/C3=[N+](C)C4=C(C=C(C=C4)S(=O)([O-])=O)C3(C)C)CCCCCC(NCCCC[C@@H]([NH3+])C(N)=O)=O)C=CC(S(=O)([O-])=O)=C2)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for sCy5DL-amide

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 7.7 10

Preparing Stock Solutions for sCy5DL-amide

The following data is based on the product molecular weight 769.97. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 12.99 mL 64.94 mL 129.88 mL
0.5 mM 2.6 mL 12.99 mL 25.98 mL
1 mM 1.3 mL 6.49 mL 12.99 mL
5 mM 0.26 mL 1.3 mL 2.6 mL

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References for sCy5DL-amide

References are publications that support the biological activity of the product.

Zhang et al (2022) Fluorescent D-amino acids for super-resolution microscopy of the bacterial cell wall. ACS Chem.Biol. 117 2418 PMID: 35994360

If you know of a relevant reference for sCy5DL-amide, please let us know.

Keywords: sCy5DL-amide, sCy5DL-amide supplier, fluorescent, D-amino, acid, FDAA, peptidoglycans, labeling, labels, labelling, amino, acids, probes, for, imaging, bacteria, SMLM, PALM, STORM, Fluorescent, Probes, Imaging, Bacteria, 7835, Tocris Bioscience

Citations for sCy5DL-amide

Citations are publications that use Tocris products.

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals