Pricing Availability   Qty

Save up to 40% on RUO Reagents with BIOSPRING24 (see details)

Description: FDAA for super-resolution microscopy of bacteria
Chemical Name: (R)-2-Ammonio-3-(6-((E)-3,3-dimethyl-5-sulfo-2-((2E,4E)-5-(1,3,3-trimethyl-5-sulfonato-3H-indol-1-ium-2-yl)penta-2,4-dien-1-ylidene)indolin-1-yl)hexanamido)propanoate
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for sCy5DA

sCy5DA is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans in live bacteria. It is efficiently incorporated into the peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into gram-negative C. crescentus. sCy5DA labelled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/665 nm.

Scientific Data

Fluorescence Imaging Vibrio cholerae cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Vibrio cholerae cells labeled with 500 μM sCy5DA

Fluorescence Imaging Bacillus subtilis cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA

Fluorescence Imaging Mycobacterium smegmatis cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Mycobacterium smegmatis cells labeled with 100 μM sCy5DA

Fluorescence Imaging Staphylococcus aureus cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Staphylococcus aureus labeled with 100 μM sCy5DA

Optical Data for sCy5DA

sCy5DA Dye Spectra
Emission Color Red
λabs 646 nm
λem 665 nm
Extinction Coefficient (ε) 197,240 M-1cm-1
Cell Permeable Yes
Application STORM

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for sCy5DA

M. Wt 728.88
Formula C35H44N4O9S2
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(NC[C@H](C([O-])=O)[NH3+])=O)/C1=C/C=C/C=C/C3=[N+](C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for sCy5DA

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 7.29 10

Preparing Stock Solutions for sCy5DA

The following data is based on the product molecular weight 728.88. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 13.72 mL 68.6 mL 137.2 mL
0.5 mM 2.74 mL 13.72 mL 27.44 mL
1 mM 1.37 mL 6.86 mL 13.72 mL
5 mM 0.27 mL 1.37 mL 2.74 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

References for sCy5DA

References are publications that support the biological activity of the product.

Zhang et al (2022) Fluorescent D-amino acids for super-resolution microscopy of the bacterial cell wall. ACS Chem.Biol. 17 2418 PMID: 35994360

If you know of a relevant reference for sCy5DA, please let us know.

Keywords: sCy5DA, sCy5DA supplier, fluorescent, D-amino, acid, FDAA, peptidoglycans, labeling, labels, labelling, amino, acids, probes, for, imaging, bacteria, SMLM, PALM, STORM, Fluorescent, Probes, Imaging, Bacteria, 7834, Tocris Bioscience

Citations for sCy5DA

Citations are publications that use Tocris products.

Currently there are no citations for sCy5DA. Do you know of a great paper that uses sCy5DA from Tocris? Please let us know.

Reviews for sCy5DA

There are currently no reviews for this product. Be the first to review sCy5DA and earn rewards!

Have you used sCy5DA?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals