sCy5DA

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Description: FDAA for super-resolution microscopy of bacteria
Chemical Name: (R)-2-Ammonio-3-(6-((E)-3,3-dimethyl-5-sulfo-2-((2E,4E)-5-(1,3,3-trimethyl-5-sulfonato-3H-indol-1-ium-2-yl)penta-2,4-dien-1-ylidene)indolin-1-yl)hexanamido)propanoate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for sCy5DA

sCy5DA is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans in live bacteria. It is efficiently incorporated into the peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into gram-negative C. crescentus. sCy5DA labelled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/665 nm.

Scientific Data

Fluorescence Imaging Vibrio cholerae cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Vibrio cholerae cells labeled with 500 μM sCy5DA

Fluorescence Imaging Bacillus subtilis cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA

Fluorescence Imaging Mycobacterium smegmatis cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Mycobacterium smegmatis cells labeled with 100 μM sCy5DA

Fluorescence Imaging Staphylococcus aureus cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Staphylococcus aureus labeled with 100 μM sCy5DA

Optical Data for sCy5DA

sCy5DA Dye Spectra
Emission Color Red
λabs 646 nm
λem 665 nm
Extinction Coefficient (ε) 197,240 M-1cm-1
Cell Permeable Yes
Application STORM

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for sCy5DA

M. Wt 728.88
Formula C35H44N4O9S2
Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key ZSTSSXALPFZNBO-HHHXNRCGSA-N
Smiles CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(NC[C@H](C([O-])=O)[NH3+])=O)/C1=C/C=C/C=C/C3=[N+](C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for sCy5DA

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.29 10

Preparing Stock Solutions for sCy5DA

The following data is based on the product molecular weight 728.88. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 13.72 mL 68.6 mL 137.2 mL
0.5 mM 2.74 mL 13.72 mL 27.44 mL
1 mM 1.37 mL 6.86 mL 13.72 mL
5 mM 0.27 mL 1.37 mL 2.74 mL

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References for sCy5DA

References are publications that support the biological activity of the product.

Zhang et al (2022) Fluorescent D-amino acids for super-resolution microscopy of the bacterial cell wall. ACS Chem.Biol. 17 2418 PMID: 35994360


If you know of a relevant reference for sCy5DA, please let us know.

Keywords: sCy5DA, sCy5DA supplier, fluorescent, D-amino, acid, FDAA, peptidoglycans, labeling, labels, labelling, amino, acids, probes, for, imaging, bacteria, SMLM, PALM, STORM, Fluorescent, Probes, Imaging, Bacteria, 7834, Tocris Bioscience

Citations for sCy5DA

Citations are publications that use Tocris products.

Currently there are no citations for sCy5DA. Do you know of a great paper that uses sCy5DA from Tocris? Please let us know.

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Literature in this Area

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