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Cat.No. 6659 - sBADA | C17H20BF2N4O6S.Na.C2HF3O2
Description: Green fluorescent D-amino acid for labeling peptidoglycans in bacteria
Chemical Name: Sodium (R)-3-(3-((2-amino-2-carboxyethyl)amino)-3-oxopropyl)-5,5-difluoro-7,9-dimethyl-2-sulfonato-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide trifluoroacetate
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity

Green sulfonated BODIPY-FL 3-amino-D-alanine (sBADA) or fluorescent D-amino acid (FFDA). Labels peptidoglycans in live bacteria. Sulfonated from of BADA with increased hydrophilicity. Excitation/emission λ ~490/510 nm.

Optical Data for sBADA

sBADA Dye Spectra
Emission Color Green
λabs 490 nm
λem 510 nm
Extinction Coefficient (ε) 49000 M-1cm-1

Technical Data

M. Wt 594.25
Formula C17H20BF2N4O6S.Na.C2HF3O2
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(NC[C@@H](N)C(O)=O)=O)C(S(=O)(O[Na])=O)=CC3=C2.OC(C(F)(F)F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 2 4

Preparing Stock Solutions

The following data is based on the product molecular weight 594.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.04 mM 42.07 mL 210.35 mL 420.7 mL
0.2 mM 8.41 mL 42.07 mL 84.14 mL
0.4 mM 4.21 mL 21.03 mL 42.07 mL
2 mM 0.84 mL 4.21 mL 8.41 mL

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References are publications that support the biological activity of the product.

Hsu et al (2017) Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 8 6313 PMID: 28989665

If you know of a relevant reference for sBADA, please let us know.

Keywords: sBADA, sBADA supplier, boron-dipyrromethene, BODIPY®, sulfonated, BODIPY®-FL, 3-amino-D-alanine, Fluorescent, D-amino, acid, FFDA, Unnatural, Amino, Acids, Probes, for, Imaging, Bacteria, 6659, Tocris Bioscience

Citations for sBADA

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Literature in this Area

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