Ro 5-3335

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Description: Core binding factor inhibitor; attenuates hematopoiesis
Alternative Names: CBFβ-Runx1 inhibitor II
Chemical Name: 7-Chloro-1,3-dihydro-5-(1H-pyrrol-2-yl)-2H-1,4-benzodiazepin-2-one
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Reviews

Biological Activity for Ro 5-3335

Ro 5-3335 is a core binding factor (CBF) inhibitor; preferentially kills human leukemia cell lines with CBF fusion proteins (IC50 = 1.1 μM). Represses RUNX1/CBFβ-dependent transactivation in reporter assays and inhibits transcriptional regulation by RUNX1 and CBFβ. Reduces leukemia burden in a mouse model. Attenuates RUNX1-dependent hematopoiesis in zebrafish embryos. Also a Tat antagonist; inhibits HIV-1 replication in vitro.

Compound Libraries for Ro 5-3335

Ro 5-3335 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for Ro 5-3335

M. Wt 259.69
Formula C13H10ClN3O
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 30195-30-3
PubChem ID 64983
InChI Key XWNMORIHKRROGW-UHFFFAOYSA-N
Smiles O=C1CN=C(C3=CC=CN3)C2=C(C=CC(Cl)=C2)N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Ro 5-3335

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 25.96 100

Preparing Stock Solutions for Ro 5-3335

The following data is based on the product molecular weight 259.69. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.85 mL 19.25 mL 38.51 mL
5 mM 0.77 mL 3.85 mL 7.7 mL
10 mM 0.39 mL 1.93 mL 3.85 mL
50 mM 0.08 mL 0.39 mL 0.77 mL

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Product Datasheets for Ro 5-3335

Certificate of Analysis / Product Datasheet
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References for Ro 5-3335

References are publications that support the biological activity of the product.

Cunningham et al (2012) Identification of benzodiazepine Ro5-3335 as an inhibitor of CBF leukemia through quantitative high throughput screen against RUNX1-CBF beta interaction. Proc.Natl.Acad.Sci.USA 109 14592 PMID: 22912405

Cupelli et al (1995) The human immunodeficiency virus type 1 Tat antagonist, Ro 5-3335, predominantly inhibits transcription initiation from the viral promoter. J.Virol. 69 2640 PMID: 7884917

Okuda et al (2001) RUNX1/AML1: a central player in hematopoiesis. Int. J. Hematol. 74 252 PMID: 11721959


If you know of a relevant reference for Ro 5-3335, please let us know.

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Keywords: Ro 5-3335, Ro 5-3335 supplier, Ro5-3335, CBF, core, binding, factor, Leukemia, inhibitors, inhibits, runt-related, transcription, 1, RUNX1, acute, myeloid, leukemia, protein, AML1, CBFβ, CBFα, hematopoiesis, HIV-1, replication, antiviral, CBFβ-Runx1, II, CBFbeta-Runx1, inhibitor, Other, Transcription, Factors, 4694, Tocris Bioscience

1 Citation for Ro 5-3335

Citations are publications that use Tocris products. Selected citations for Ro 5-3335 include:

Luo et al (2016) Runt-related Transcription Factor 1 (RUNX1) Binds to p50 in Macrophages and Enhances TLR4-triggeredInflammation and Septic Shock. Journal of Biological Chemistry PMID: 27573239


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