Ro 5-3335

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Cat.No. 4694 - Ro 5-3335 | C13H10ClN3O | CAS No. 30195-30-3
Description: Core binding factor inhibitor; attenuates hematopoiesis
Alternative Names: CBFβ-Runx1 inhibitor II
Chemical Name: 7-Chloro-1,3-dihydro-5-(1H-pyrrol-2-yl)-2H-1,4-benzodiazepin-2-one
Purity: ≥98% (HPLC)
Citations (1)

Biological Activity

Core binding factor (CBF) inhibitor; preferentially kills human leukemia cell lines with CBF fusion proteins (IC50 = 1.1 μM). Represses RUNX1/CBFβ-dependent transactivation in reporter assays and inhibits transcriptional regulation by RUNX1 and CBFβ. Reduces leukemia burden in a mouse model. Attenuates RUNX1-dependent hematopoiesis in zebrafish embryos. Also a Tat antagonist; inhibits HIV-1 replication in vitro.

Technical Data

M. Wt 259.69
Formula C13H10ClN3O
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 30195-30-3
PubChem ID 64983
Smiles O=C1CN=C(C3=CC=CN3)C2=C(C=CC(Cl)=C2)N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 25.96 100

Preparing Stock Solutions

The following data is based on the product molecular weight 259.69. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.85 mL 19.25 mL 38.51 mL
5 mM 0.77 mL 3.85 mL 7.7 mL
10 mM 0.39 mL 1.93 mL 3.85 mL
50 mM 0.08 mL 0.39 mL 0.77 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References are publications that support the biological activity of the product.

Cunningham et al (2012) Identification of benzodiazepine Ro5-3335 as an inhibitor of CBF leukemia through quantitative high throughput screen against RUNX1-CBF beta interaction. Proc.Natl.Acad.Sci.USA 109 14592 PMID: 22912405

Cupelli et al (1995) The human immunodeficiency virus type 1 Tat antagonist, Ro 5-3335, predominantly inhibits transcription initiation from the viral promoter. J.Virol. 69 2640 PMID: 7884917

Okuda et al (2001) RUNX1/AML1: a central player in hematopoiesis. Int. J. Hematol. 74 252 PMID: 11721959

If you know of a relevant reference for Ro 5-3335, please let us know.

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1 Citation for Ro 5-3335

Citations are publications that use Tocris products. Selected citations for Ro 5-3335 include:

Luo et al (2016) Runt-related Transcription Factor 1 (RUNX1) Binds to p50 in Macrophages and Enhances TLR4-triggeredInflammation and Septic Shock. Journal of Biological Chemistry PMID: 27573239

Do you know of a great paper that uses Ro 5-3335 from Tocris? Please let us know.

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