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Biological Activity for Rf470DL
Rf470DL is a blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria. Rf470DL fluoresces only when it becomes incorporated into the peptidoglycan, enabling no wash experiments. Excitation/emission λ ~470/620 nm; quantum yield = 0.042; extinction coefficient 33,106 M-1 cm-1 (measured in PBS containing 50% glycerol).
Sold under license from Indiana University. U.S. patent application number 16/967,874.
Optical Data for Rf470DL
|Extinction Coefficient (ε)||33106 M-1cm-1|
|Quantum Yield (φ)||0.042|
|Application||Fluorogenic probe for imaging bacterial cell wall biosynthesis (PG biosynthesis)|
Technical Data for Rf470DL
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Rf470DL
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Rf470DL
The following data is based on the product molecular weight 547.07. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.83 mL||9.14 mL||18.28 mL|
|5 mM||0.37 mL||1.83 mL||3.66 mL|
|10 mM||0.18 mL||0.91 mL||1.83 mL|
|50 mM||0.04 mL||0.18 mL||0.37 mL|
References for Rf470DL
References are publications that support the biological activity of the product.
Hsu et al (2019) Fluorogenic D-amino acids enable real-time monitoring of peptidoglycan biosynthesis and high-throughput transpeptidation assays. Nat.Chem. 11 335 PMID: 30804500
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Citations for Rf470DL
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Fluorescent Probes and Dyes Research Product Guide
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