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Description: Blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name: N6-[3-[6-[5-[2-Carboxy-2-cyanoethenyl]-2-thienyl]-3,4-dihydro-1(2H)-quinolinyl]-1-oxopropyl]-D-lysine hydrochloride
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Rf470DL

Rf470DL is a blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria. Rf470DL fluoresces only when it becomes incorporated into the peptidoglycan, enabling no wash experiments. Excitation/emission λ ~470/620 nm; quantum yield = 0.042; extinction coefficient 33,106 M-1 cm-1 (measured in PBS containing 50% glycerol).

Licensing Information

Sold under license from Indiana University. U.S. patent application number 16/967,874.

Optical Data for Rf470DL

Emission Color Red
λabs 470 nm
λem 640 nm
Extinction Coefficient (ε) 33106 M-1cm-1
Quantum Yield (φ) 0.042
Application Fluorogenic Probe for Imaging Bacterial Cell Wall Biosynthesis (PG Biosynthesis)

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Technical Data for Rf470DL

M. Wt 547.07
Formula C26H30N4O5S.HCl
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles N[C@@H](C(O)=O)CCCCNC(CCN1CCCC2=CC(C3=CC=C(/C=C(C(O)=O)\C#N)S3)=CC=C12)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Rf470DL

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 54.71 100

Preparing Stock Solutions for Rf470DL

The following data is based on the product molecular weight 547.07. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.83 mL 9.14 mL 18.28 mL
5 mM 0.37 mL 1.83 mL 3.66 mL
10 mM 0.18 mL 0.91 mL 1.83 mL
50 mM 0.04 mL 0.18 mL 0.37 mL

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References for Rf470DL

References are publications that support the biological activity of the product.

Hsu et al (2019) Fluorogenic D-amino acids enable real-time monitoring of peptidoglycan biosynthesis and high-throughput transpeptidation assays. Nat.Chem. 11 335 PMID: 30804500

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Citations for Rf470DL

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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