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ReZolve-ER™

Cat. No. 6640 Submit a Review Datasheet / COA / SDS
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Cat.No. 6640 - ReZolve-ER | C21H12N7O3Re | CAS No. 1404104-40-0
Description: Fluorescent ER and plasma membrane stain
Chemical Name: (OC-6-33)-Tricarbonyl(1,10-phenanthroline-κN1,κN10)[4-(2H-terazol-5-yl-κN2)pyridinato]Rhenium
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Literature
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Fluorescent endoplasmic reticulum and plasma membrane stain. Exhibits rapid uptake in live cells. Displays low cytotoxicity and is resistant to photobleaching. Suitable for imaging live and fixed cells in short and long time course experiments. Compatible with fluorescent microscopy using single photon excitation at 403 nm or two-photon excitation between 800 and 830 nm. Excitation/emission λ: 350-405 nm / 500-650 nm.

Application of ReZolve-ER™ on PNT2 prostate epithelial cells

PNT2 prostate epithelial cell line stained with ReZolve-ER™ PNT2 prostate epithelial cell line stained with ReZolve-ER™ (green) and CellMask (purple)*

Left: PNT2 prostate epithelial cells stained with ReZolve-ER™. Right: PNT2 prostate epithelial cells stained with ReZolve-ER™ (green) and CellMask (purple)*.

Cells are incubated with growth media containing 20 μM ReZolve-ER™ for 10-20 min before imaging. Cells were viewed using a Nikon+A1 confocal microscope, ReZolve-ER™ is excited at 403 nm and emission is collected using a 525 nm GaaS PMT detect.

*CellMask was purchased from Thermo Fisher

Technical Data

M. Wt 596.58
Formula C21H12N7O3Re
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 1404104-40-0
PubChem ID 137700480
InChI Key RRCDXVYOOGUGOF-UHFFFAOYSA-N
Smiles [O]#C[Re+](C#[O])([N]1=CC=CC2=CC=C3C4=C12)([N]4=CC=C3)([N-]5N=C(C6=CC=NC=C6)N=N5)C#[O]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 5.97 10

Preparing Stock Solutions

The following data is based on the product molecular weight 596.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 16.76 mL 83.81 mL 167.62 mL
0.5 mM 3.35 mL 16.76 mL 33.52 mL
1 mM 1.68 mL 8.38 mL 16.76 mL
5 mM 0.34 mL 1.68 mL 3.35 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Bader et al (2016) Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time. FEBS Lett. 590 3051 PMID: 27543777

Gillam et al (2018) Bright lights down under: Metal ion complexes turning the spotlight on metabolic processes at the cellular level. Coordination Chem.Rev. 375 234

If you know of a relevant reference for ReZolve-ER™, please let us know.

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Keywords: ReZolve-ER, ReZolve-ER supplier, endoplasmic, reticulum, plasma, membrane, dyes, stains, live, cells, fluorescent, Organelle, and, Cell, Structure, Stains, 6640, Tocris Bioscience

Citations for ReZolve-ER™

Citations are publications that use Tocris products.

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Protocols for ReZolve-ER™

The following protocol features additional information for the use of ReZolve-ER™ (Cat. No. 6640).

Literature in this Area

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