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Fluorescent endoplasmic reticulum and plasma membrane stain. Exhibits rapid uptake in live cells. Displays low cytotoxicity and is resistant to photobleaching. Suitable for imaging live and fixed cells in short and long time course experiments. Compatible with fluorescent microscopy using single photon excitation at 403 nm or two-photon excitation between 800 and 830 nm. Excitation/emission λ: 350-405 nm / 500-650 nm.
Application of ReZolve-ER™ on PNT2 prostate epithelial cells
Left: PNT2 prostate epithelial cells stained with ReZolve-ER™. Right: PNT2 prostate epithelial cells stained with ReZolve-ER™ (green) and CellMask (purple)*.
Cells are incubated with growth media containing 20 μM ReZolve-ER™ for 10-20 min before imaging. Cells were viewed using a Nikon+A1 confocal microscope, ReZolve-ER™ is excited at 403 nm and emission is collected using a 525 nm GaaS PMT detect.
*CellMask was purchased from Thermo Fisher
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 596.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||16.76 mL||83.81 mL||167.62 mL|
|0.5 mM||3.35 mL||16.76 mL||33.52 mL|
|1 mM||1.68 mL||8.38 mL||16.76 mL|
|5 mM||0.34 mL||1.68 mL||3.35 mL|
References are publications that support the biological activity of the product.
Bader et al (2016) Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time. FEBS Lett. 590 3051 PMID: 27543777
Gillam et al (2018) Bright lights down under: Metal ion complexes turning the spotlight on metabolic processes at the cellular level. Coordination Chem.Rev. 375 234
If you know of a relevant reference for ReZolve-ER™, please let us know.
Keywords: ReZolve-ER, ReZolve-ER supplier, endoplasmic, reticulum, plasma, membrane, dyes, stains, live, cells, fluorescent, Organelle, and, Cell, Structure, Stains, 6640, Tocris Bioscience
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Protocols for ReZolve-ER™
The following protocol features additional information for the use of ReZolve-ER™ (Cat. No. 6640).
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