Purvalanol A

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Cat.No. 1580 - Purvalanol A | C19H25ClN6O | CAS No. 212844-53-6
Description: Cyclin-dependent kinase inhibitor
Alternative Names: NG 60
Chemical Name: (2R)-2-[[6-[(3-Chlorophenyl)amino]-9-(1-methylethyl)-9H-purin-2-yl]amino]-3-methyl-1-butanol
Purity: ≥98% (HPLC)
Datasheet
Citations (3)
Literature

Biological Activity

Cyclin-dependent kinase inhibitor. IC50 values are 4, 70, 35, 850 and 75 nM for cdc2/cyclin B, cdk2/cyclin A, cdk2/cyclin E, cdk4/cyclin D1 and cdk5-p35 respectively. Reversibly arrests synchronised cells in G1 and G2, and inhibits cell proliferation and cell death.

Licensing Information

Sold under license from the Regents of the University of California

Compound Libraries

Purvalanol A is also offered as part of the Tocriscreen Plus and Tocriscreen Kinase Inhibitor Toolbox I. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 388.9
Formula C19H25ClN6O
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 212844-53-6
PubChem ID 456214
InChI Key PMXCMJLOPOFPBT-HNNXBMFYSA-N
Smiles ClC1=CC=CC(NC2=C3C(N(C(C)C)C=N3)=NC(N[C@H]([C@@H](C)C)CO)=N2)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
ethanol 19.45 50

Preparing Stock Solutions

The following data is based on the product molecular weight 388.9. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.57 mL 12.86 mL 25.71 mL
5 mM 0.51 mL 2.57 mL 5.14 mL
10 mM 0.26 mL 1.29 mL 2.57 mL
50 mM 0.05 mL 0.26 mL 0.51 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Bain et al (2003) The specificities of protein kinase inhibitors: an update. Biochem.J. 371 199 PMID: 12534346

Gray et al (1998) Exploiting chemical libraries, structure, and genomics in the search for kinase inhibitors. Science 281 533 PMID: 9677190

Gray et al (1999) ATP-site directed inhibitors of cyclin-dependent kinases. Curr.Med.Chem. 6 859 PMID: 10495356

Villerbu et al (2002) Cellular effects of purvalanol A: a specific inhibitor of cyclin-dependent kinase activities. Int.J.Cancer 97 761 PMID: 11857351


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Keywords: Purvalanol A, supplier, Cyclin-dependent, protein, kinases, inhibitors, inhibits, Cdk, PurvalanolA, NG60, NG, 60, Cyclin-dependent, Kinase, Tocris Bioscience

3 Citations for Purvalanol A

Citations are publications that use Tocris products. Selected citations for Purvalanol A include:

Ozl├╝ et al (2010) Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition. Mol Cell Proteomics 9 336 PMID: 19786723

Rowe et al (2010) Compounds that target host cell proteins prevent varicella-zoster virus replication in culture, ex vivo, and in SCID-Hu mice. Antiviral Res 86 276 PMID: 20307580

Pennati et al (2005) Potentiation of paclitaxel-induced apoptosis by the novel cyclin-dependent kinase inhibitor NU6140: a possible role for survivin down-regulation. Mol Cancer Ther 4 1328 PMID: 16170024


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Literature in this Area

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Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for Purvalanol A

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