Pricing Availability   Qty
Description: O-GlcNAcase and β-hexosaminidase inhibitor
Chemical Name: O-(2-Acetamido-2-deoxy-D-glucopyranosylidene)amino-Z-N-phenylcarbamate
Purity: ≥98% (HPLC)
Citations (2)

Biological Activity for (Z)-Pugnac

(Z)-Pugnac is an O-GlcNAc-β-N-acetylglucosaminidase (O-GlcNAcase) and β-hexosaminidase inhibitor (Ki values are 46 and 36 nM respectively) that increases O-GlcNAc levels ~ 2-fold in HT29 cells. Z-linked isomer is more potent than the E isomer.

Technical Data for (Z)-Pugnac

M. Wt 353.33
Formula C15H19N3O7
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 132489-69-1
PubChem ID 6074876
Smiles OC(C1NC(C)=O)C(O)C(CO)O/C1=N\OC(NC2=CC=CC=C2)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for (Z)-Pugnac

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 35.33 100

Preparing Stock Solutions for (Z)-Pugnac

The following data is based on the product molecular weight 353.33. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.83 mL 14.15 mL 28.3 mL
5 mM 0.57 mL 2.83 mL 5.66 mL
10 mM 0.28 mL 1.42 mL 2.83 mL
50 mM 0.06 mL 0.28 mL 0.57 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for (Z)-Pugnac

Certificate of Analysis / Product Datasheet
Select another batch:

References for (Z)-Pugnac

References are publications that support the biological activity of the product.

Dong and Hart (1994) Purification and characterization of an O-GlcNAc selective N-acetyl-β-D-glucosaminidase from rat spleen cytosol. J.Biol.Chem. 269 19321 PMID: 8034696

Macauley et al (2005) O-GlcNAcase uses substrate-assisted catalysis. J.Biol.Chem. 280 25313 PMID: 15795231

Perreira et al (2006) Inhibition of O-GlcNAcase by PUGNAc is dependent upon the oxime stereochemistry. Bioorg.Med.Chem. 14 837 PMID: 16214344

If you know of a relevant reference for (Z)-Pugnac, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Other Hydrolase Inhibitors

Keywords: Pugnac, Pugnac supplier, O-GlcNAcase, β-hexosaminidase, beta-hexosaminidase, O-GlcNAc-β-N-acetylglucosaminidase, O-GlcNAc-beta-N-acetylglucosaminidase, inhibitors, inhibits, Hydrolases, Other, 3384, Tocris Bioscience

2 Citations for (Z)-Pugnac

Citations are publications that use Tocris products. Selected citations for (Z)-Pugnac include:

Jo et al (2016) O-GlcNAcylation of ATG4B positively regulates autophagy by increasing its hydroxylase activity. Oncotarget 7 57186 PMID: 27527864

Parween et al (2018) Higher O-GlcNAc Levels Are Associated with Defects in Progenitor Proliferation and Premature Neuronal Differentiation during in-Vitro Human Embryonic Cortical Neurogenesis. Front Cell Neurosci 11 415 PMID: 29311838

Do you know of a great paper that uses (Z)-Pugnac from Tocris? Please let us know.

Reviews for (Z)-Pugnac

There are currently no reviews for this product. Be the first to review (Z)-Pugnac and earn rewards!

Have you used (Z)-Pugnac?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review