proTAME

Pricing Availability   Qty

Save up to 40% on RUO Reagents with BIOSPRING24 (see details)

Description: Cell-permeable APC/C inhibitor
Chemical Name: Methyl (2S)-2-[[(4-methylphenyl)sulfonyl]amino]-9,13-dioxo-14-phenyl-7-[[[[(2-phenylacetyl)oxy]methoxy]carbonyl]amino]-10,12-dioxa-6,8-diazatetradec-6-enoate
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for proTAME

ProTAME is a cell-permeable APC/C (anaphase-promoting complex/cyclosome) inhibitor. It is converted to TAME (Tosyl-L-Arginine Methyl Ester) by intracellular esterases. It induces cell cycle arrest in metaphase followed by cell death.

Technical Data for proTAME

M. Wt 726.75
Formula C34H38N4O12S
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 1362911-19-0
PubChem ID 56924780
InChI Key MHYOVHULCQSDRZ-NDEPHWFRSA-N
Smiles O=C([C@@H](NS(=O)(C1=CC=C(C=C1)C)=O)CCC/N=C(NC(OCOC(CC2=CC=CC=C2)=O)=O)/NC(OCOC(CC3=CC=CC=C3)=O)=O)OC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for proTAME

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 36.34 50

Preparing Stock Solutions for proTAME

The following data is based on the product molecular weight 726.75. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.75 mL 13.76 mL 27.52 mL
2.5 mM 0.55 mL 2.75 mL 5.5 mL
5 mM 0.28 mL 1.38 mL 2.75 mL
25 mM 0.06 mL 0.28 mL 0.55 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for proTAME

References are publications that support the biological activity of the product.

Zeng et al (2010) Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. Cancer Cell 18 382 PMID: 20951947


If you know of a relevant reference for proTAME, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Mitosis Inhibitors

Keywords: proTAME, proTAME supplier, cell, cycle, meiosis, mitosis, anaphase, promoting, complex, cyclosome, metaphase, TAME, apoptosis, cell-permeable, Mitosis, 7734, Tocris Bioscience

Citations for proTAME

Citations are publications that use Tocris products.

Currently there are no citations for proTAME. Do you know of a great paper that uses proTAME from Tocris? Please let us know.

Reviews for proTAME

There are currently no reviews for this product. Be the first to review proTAME and earn rewards!

Have you used proTAME?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Cell Cycle & DNA Damage Repair Poster

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. This poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.