Inhibits p53 binding to mitochondria by reducing its affinity for antiapoptotic proteins Bcl-2 and Bcl-XL. Displays no effect on the transactivational or cell cycle checkpoint control function of p53. Potentially increases reprogramming efficiency of human somatic cells to induced pluripotent stem cells (iPSCs) by silencing p53. Reduces cell death induced by γ-radiation in vitro and protects mice from doses of radiation that cause lethal hematopoietic syndrome. Selectively inhibits heat shock protein 70 (HSP70) activity.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 181.21. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||5.52 mL||27.59 mL||55.18 mL|
|5 mM||1.1 mL||5.52 mL||11.04 mL|
|10 mM||0.55 mL||2.76 mL||5.52 mL|
|50 mM||0.11 mL||0.55 mL||1.1 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Strom et al (2006) Small-molecule inhibitor of p53 binding to mitochondria protects mice from gamma radiation. Nat.Chem.Biol. 2 474 PMID: 16862141
Leu et al (2009) A small molecule inhibitor of inducible heat shock protein 70 (HSP70). Mol.Cell 36 15 PMID: 19818706
Kawamura et al (2009) Linking the p53 tumor suppressor pathway to somatic cell reprogramming. Nature 460 1140 PMID: 19668186
Steele et al (2009) 2-phenylacetylenesulfonamide (PAS) induces p53-independent apoptotic killing of B-chronic lymphocytic leukemia (CLL) cells. Blood 114 1217 PMID: 19515722
If you know of a relevant reference for Pifithrin-μ, please let us know.
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Keywords: inhibitors inhibits p53-mitochondrial binding Transcription Factors MDM2 p14ARF Pifithrin-mu NSC303580 NSC 303580 p53
4 Citations for Pifithrin-μ
Citations are publications that use Tocris products. Selected citations for Pifithrin-μ include:
Blatzer et al (2015) Blocking Hsp70 Enhances the Efficiency of Amphotericin B Treatment against Resistant Aspergillus terreus Strains. Antimicrob Agents Chemother 59 3778 PMID: 25870060
Xia et al (2014) EZH2 silencing with RNA interference induces G2/M arrest in human lung cancer cells in vitro. Biomed Res Int 2014 348728 PMID: 24745014
Wang et al (2013) Active Constituents from Liriope platyphylla Root against Cancer Growth In Vitro. PLoS One 2013 857929 PMID: 23762164
Kojima et al (2010) The novel tryptamine derivative JNJ-26854165 induces wild-type p53- and E2F1-mediated apoptosis in acute myeloid and lymphoid leukemias. Mol Cancer Ther 9 2545 PMID: 20736344
Do you know of a great paper that uses Pifithrin-μ from Tocris? If so please let us know.
Literature in this Area
Cell Cycle & DNA Damage Repair Poster
In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.