PBIT

Pricing Availability Delivery Time Qty
Cat.No. 5930 - PBIT | C14H11NOS | CAS No. 2514-30-9
Description: JARID1 (Jumonji AT-Rich Interactive Domain 1) inhibitor
Chemical Name: 2-(4-Methylphenyl)-1,2-benzisothiazol-3(2H)-one
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

JARID1 (Jumonji AT-Rich Interactive Domain 1) inhibitor (IC50 values are 3, 4.9 and 6 μM for JARID1B, 1A and 1C, respectively). Selective for JARID1 over UTX and JMJD3. Increases levels of methylated H3K4 in JARIDB1-transfected HeLa cells and MCF7 cells. Inhibits proliferation of breast cancer cell lines expressing high levels of JARIDB1.

Technical Data

M. Wt 241.31
Formula C14H11NOS
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 2514-30-9
PubChem ID 935415
InChI Key KRXMYBAZKJBJAB-UHFFFAOYSA-N
Smiles CC1=CC=C(N2SC3=C(C2=O)C=CC=C3)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 24.13 100
ethanol 4.83 20

Preparing Stock Solutions

The following data is based on the product molecular weight 241.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.14 mL 20.72 mL 41.44 mL
5 mM 0.83 mL 4.14 mL 8.29 mL
10 mM 0.41 mL 2.07 mL 4.14 mL
50 mM 0.08 mL 0.41 mL 0.83 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Sayegh et al (2013) Identification of small molecule inhibitors of Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase by a sensitive high throughput screen. J.Biol.Chem. 288 9408 PMID: 23408432


If you know of a relevant reference for PBIT, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Histone Demethylase Inhibitors

Keywords: PBIT, supplier, Jumonji, AT-Rich, Interactive, Domain, 1, JARID1B, histone, lysine, demethylase, inhibitors, inhibits, breast, cancer, Histone, Demethylases, Histone, Demethylases, Tocris Bioscience

Citations for PBIT

Citations are publications that use Tocris products.

Currently there are no citations for PBIT. Do you know of a great paper that uses PBIT from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Epigenetics

Epigenetics Scientific Review

Written by Susanne Müller-Knapp and Peter J. Brown, this review gives an overview of the development of chemical probes for epigenetic targets, as well as the impact of these tool compounds being made available to the scientific community. In addition, their biological effects are also discussed. Epigenetic compounds available from Tocris are listed.

Epigenetics

Epigenetics Research Bulletin

Produced by Tocris and updated in 2014, the epigenetics research bulletin gives an introduction into mechanisms of epigenetic regulation, and highlights key Tocris products for epigenetics targets including:

  • Bromodomains
  • DNA Methyltransferases
  • Histone Deacetylases
  • Histone Demethylases
  • Histone Methyltransferases

Pathways for PBIT

Protocols

TODO: Add Protocols