Pricing Availability   Qty
Description: TLR1/2 agonist; induces cytokine production
Purity: ≥95% (HPLC)
Citations (3)
Reviews (2)
Pathways (1)

Biological Activity for Pam3CSK4

Pam3CSK4 is a toll-like receptor 1/2 (TLR1/2) agonist; induces production of TNF-α and IL-6 in macrophages. Stimulates phosphorylation of p100/p110 and p60 in granulocytic-differentiated HL-60 cells. Promotes differentiation of naive CD4+ T cells into Th17 cells.

Technical Data for Pam3CSK4

M. Wt 1510.24
Formula C81H156N10O13S
Sequence CSKKKK

(Modifications: Cys-1 = S-[2,3-Bis(palmitoyloxy)-(2-R/S)-propyl]-N-palmitoyl]-Cys

Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 112208-00-1
PubChem ID 130704

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Pam3CSK4

Solubility Soluble to 1 mg/ml in 50% Ethanol / water

Product Datasheets for Pam3CSK4

Certificate of Analysis / Product Datasheet
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References for Pam3CSK4

References are publications that support the biological activity of the product.

St Paul et al (2012) Toll-like receptor ligands induce the expression of IF.-gamma and interleukin-17 in chicken CD4+ T cells. BMC Res.Notes 1 616 PMID: 23116495

Offermanns et al (1992) Lipopeptides are effective stimulators of tyrosine phosphorylation in human myeloid cells. Biochem.J. 282 551 PMID: 1312332

Caproni et al (2012) MF59 and Pam3CSK4 boost adaptive responses to influenza subunit vaccine through an IFN type I-independent mechanism of action. J.Immunol. 188 3088 PMID: 22351935

If you know of a relevant reference for Pam3CSK4, please let us know.

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Keywords: Pam3CSK4, Pam3CSK4 supplier, toll, like, receptors, TLRs, agonists, ligands, induces, cytokines, production, IFNg, TNFa, TNFalpha, IL-6, interleukin, Toll-like, Receptors, 4633, Tocris Bioscience

3 Citations for Pam3CSK4

Citations are publications that use Tocris products. Selected citations for Pam3CSK4 include:

Valerie G et al (2022) Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence. Cell Death Differ 29 1267-1282 PMID: 34916628

Tomio et al (2020) Dietary Fiber Pectin Ameliorates Experimental Colitis in a Neutral Sugar Side Chain-Dependent Manner. Front Immunol 10 2979 PMID: 31921214

Alicja et al (2020) Integrative analysis suggests cell type-specific decoding of NF-κB dynamics. Sci Signal 13 PMID: 32098801

Do you know of a great paper that uses Pam3CSK4 from Tocris? Please let us know.

Reviews for Pam3CSK4

Average Rating: 5 (Based on 2 Reviews.)

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Pam3CSK4 inducer works great.
By Xiaogang Wang on 06/21/2021
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: differentiated human macrophages

We used 50 ng/ml of Pam3CSK4 to treat differentiated human macrophages for 2 h at 37C and measured the IL-6 production in culture supernatants of macrophages. As shown in the attached image, Pam3CSK4 treatment, but not untreated cells, significantly induced IL-6 production in human macrophages.

review image

TLR 1/2 agonist.
By Tahmineh Safaie on 01/22/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Raw-Blue

Raw-Blue Cell lines were treated with 125 ng/ml of PAM3CSK4 and the level of SEAP was measured. SEAP level correlates with NFkB activation

review image

Pathways for Pam3CSK4

Toll-like Receptor Signaling Pathway

Toll-like Receptor Signaling Pathway

TLR signaling is involved in the cellular response to threatening molecules such as bacteria and viruses. It results in an inflammatory and immmunological response.