NS 3728

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Cat.No. 6973 - NS 3728 | C16H9BrF6N6O | CAS No. 265646-85-3
Description: VRAC blocker
Alternative Names: Endovion
Chemical Name: N-[3,5-bis(Trifluoromethyl)phenyl]-N'-[4-bromo-2-(2H-tetrazol-5-yl)phenyl]urea
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews

Biological Activity

NS 3728 is a volume-regulated anion channel (VRAC) blocker (IC50 = 460 nM). Blocks red blood cell chloride conductance (IC50 = 0.6 μM). NS 3728 also inhibits Ca2+-activated Cl- current (CaCC) by anoctamin (ANO) in an apparently voltage-dependent manner (IC50 against ANO1 = 2.1 and 0.7 μM at -95 and +50 mV, respectively).

Technical Data

M. Wt 495.18
Formula C16H9BrF6N6O
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 265646-85-3
PubChem ID 10005966
InChI Key AEFYFGMSRKDXHZ-UHFFFAOYSA-N
Smiles FC(F)(C1=CC(C(F)(F)F)=CC(NC(NC2=C(C3=NN=NN3)C=C(C=C2)Br)=O)=C1)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 49.52 100
ethanol 24.76 50

Preparing Stock Solutions

The following data is based on the product molecular weight 495.18. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.02 mL 10.1 mL 20.19 mL
5 mM 0.4 mL 2.02 mL 4.04 mL
10 mM 0.2 mL 1.01 mL 2.02 mL
50 mM 0.04 mL 0.2 mL 0.4 mL

Molarity Calculator

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Reconstitution Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Helix et al (2003) Inhibition of the endogenous volume-regulated anion channel (VRAC) in HEK293 cells by acidic di-aryl-ureas. J.Membr.Biol. 196 83 PMID: 14724745

Kjaer et al (2009) Chloride channel blockers inhibit iNOS expression and NO production in IFNgamma-stimulated microglial BV2 cells. Brain Res. 1281 24 PMID: 19446535

Godwin et al (2019) E-cadherin-deficient cells have synthetic lethal vulnerabilities in plasma membrane organisation, dynamics and function. Gastric Cancer 22 273 PMID: 30066183

Sauter et al (2015) ANO1 (TMEM16A) in pancreatic ductal adenocarcinoma (PDAC). Pflugers Arch. 467 1495 PMID: 25163766


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Keywords: NS 3728, NS 3728 supplier, NS3728, volume-regulated, anion, channel, VRAC, blockers, Endovion, Other, Chloride, Channels, 6973, Tocris Bioscience

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