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Submit ReviewNS 309
Description: Positive modulator of KCa2 (SK) and KCa3.1 (IK) channels
Chemical Name: 6,7-Dichloro-1H-indole-2,3-dione 3-oxime
Purity: ≥98% (HPLC)
Biological Activity for NS 309
NS 309 is a positive modulator of small- and intermediate- conductance Ca2+-activated K+ channels (KCa2 and KCa3.1 channels); increases Ca2+ sensitivity. Displays no activity at BK channels.
Technical Data for NS 309
| M. Wt | 231.04 |
| Formula | C8H4Cl2N2O2 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 18711-16-5 |
| PubChem ID | 11637204 |
| InChI Key | CVOUSAVHMDXCKG-UHFFFAOYSA-N |
| Smiles | ClC1=C2C(C(C(N2)=O)=NO)=CC=C1Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for NS 309
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 23.1 | 100 |
Preparing Stock Solutions for NS 309
The following data is based on the product molecular weight 231.04. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.33 mL | 21.64 mL | 43.28 mL |
| 5 mM | 0.87 mL | 4.33 mL | 8.66 mL |
| 10 mM | 0.43 mL | 2.16 mL | 4.33 mL |
| 50 mM | 0.09 mL | 0.43 mL | 0.87 mL |
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Product Datasheets for NS 309
References for NS 309
References are publications that support the biological activity of the product.
Grgic et al (2009) Endothelial Ca2+-activated K+ channels in normal and impaired EDHF-dilator responses - relevance to cardiovascular pathologies and drug discovery. Br.J.Pharmacol. 157 509 PMID: 19302590
Strobaek et al (2004) Activation of human IK and SK Ca2+-activated K+ channels by NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime). Biochim.Biophys.Acta 1665 1 PMID: 15471565
Morimura et al (2006) Voltage-dependent Ca2+-channel block by openers of intermediate and small conductance Ca2+-activated K+ channels in urinary bladder smooth muscle cells. J.Pharm.Sci. 100 237 PMID: 16518073
If you know of a relevant reference for NS 309, please let us know.
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Keywords: NS 309, NS 309 supplier, NS309, Ca2+-activated, potassium, K+, KCa, channels, activators, positive, modulators, small, intermediate, conductance, SK, IK, KCa2, KCa3, Ca2+-Activated, Potassium, Channels, 3895, Tocris Bioscience
10 Citations for NS 309
Citations are publications that use Tocris products. Selected citations for NS 309 include:
Sarah Y et al (2020) Site-specific opening of the blood-brain barrier by extracellular histones. J Neuroinflammation 17 281 PMID: 32962721
Zhang et al (2015) Molecular overlap in the regulation of SK channels by small molecules and phosphoinositides. Neurochem Int 1 e1500008 PMID: 26366439
Hakim et al (2018) Electrical dynamics of isolated cerebral and skeletal muscle endothelial tubes: Differential roles of G-protein-coupled receptors and K+ channels. Pharmacol Res Perspect 6 e00391 PMID: 29636977
Brant E et al (2021) Brain endothelial cell TRPA1 channels initiate neurovascular coupling. Elife 10 PMID: 33635784
Brant E et al (2021) Endothelial pannexin 1-TRPV4 channel signaling lowers pulmonary arterial pressure in mice. Elife 10 PMID: 34490843
Erik J et al (2021) Methyl-Beta-Cyclodextrin Restores KIR Channel Function in Brain Endothelium of Female Alzheimer's Disease Mice. J Alzheimers Dis Rep 5 693-703 PMID: 34755043
Hipólito et al (2015) In vivo activation of the SK channel in the spinal cord reduces the NMDA receptor antagonist dose needed to produce antinociception in an inflammatory pain model. Pain 156 849 PMID: 25734988
D'Alessandro et al (2013) KCa3.1 channels are involved in the infiltrative behavior of glioblastoma in vivo. Cell Death Dis 4 e773 PMID: 23949222
Wong et al (2014) Sex differences in endothelial function in porcine coronary arteries: a role for H2O2 and gap junctions?. Br J Pharmacol 171 2751 PMID: 24467384
Oliveira et al (2010) Altered expression and function of small-conductance (SK) Ca(2+)-activated K+ channels in PilC-treated epileptic rats. Brain Res 1348 187 PMID: 20553876
Do you know of a great paper that uses NS 309 from Tocris? Please let us know.
Reviews for NS 309
Average Rating: 5 (Based on 1 Review.)
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NS 309 worked nicely on experimental conditions.
By
Anonymous on 10/29/2018
Assay Type: In Vitro
Species: Human
NS 309 is a potent Kca channel activator. I worked with 10,20,30,40,50,80,100 micrograms dissolved in DMSO. The product worked nicely in experimental conditions and would suggest to others researching Kca channel activation.
