NS 309

Pricing Availability   Qty
Description: Positive modulator of KCa2 (SK) and KCa3.1 (IK) channels
Chemical Name: 6,7-Dichloro-1H-indole-2,3-dione 3-oxime
Purity: ≥98% (HPLC)
Datasheet
Citations (10)
Reviews (1)

Biological Activity for NS 309

NS 309 is a positive modulator of small- and intermediate- conductance Ca2+-activated K+ channels (KCa2 and KCa3.1 channels); increases Ca2+ sensitivity. Displays no activity at BK channels.

Technical Data for NS 309

M. Wt 231.04
Formula C8H4Cl2N2O2
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 18711-16-5
PubChem ID 11637204
InChI Key CVOUSAVHMDXCKG-UHFFFAOYSA-N
Smiles ClC1=C2C(C(C(N2)=O)=NO)=CC=C1Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NS 309

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 23.1 100

Preparing Stock Solutions for NS 309

The following data is based on the product molecular weight 231.04. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.33 mL 21.64 mL 43.28 mL
5 mM 0.87 mL 4.33 mL 8.66 mL
10 mM 0.43 mL 2.16 mL 4.33 mL
50 mM 0.09 mL 0.43 mL 0.87 mL

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Product Datasheets for NS 309

Certificate of Analysis / Product Datasheet
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References for NS 309

References are publications that support the biological activity of the product.

Grgic et al (2009) Endothelial Ca2+-activated K+ channels in normal and impaired EDHF-dilator responses - relevance to cardiovascular pathologies and drug discovery. Br.J.Pharmacol. 157 509 PMID: 19302590

Strobaek et al (2004) Activation of human IK and SK Ca2+-activated K+ channels by NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime). Biochim.Biophys.Acta 1665 1 PMID: 15471565

Morimura et al (2006) Voltage-dependent Ca2+-channel block by openers of intermediate and small conductance Ca2+-activated K+ channels in urinary bladder smooth muscle cells. J.Pharm.Sci. 100 237 PMID: 16518073


If you know of a relevant reference for NS 309, please let us know.

View Related Products by Product Action

View all Calcium-Activated Potassium (KCa) Channel Modulators

Keywords: NS 309, NS 309 supplier, NS309, Ca2+-activated, potassium, K+, KCa, channels, activators, positive, modulators, small, intermediate, conductance, SK, IK, KCa2, KCa3, Ca2+-Activated, Potassium, Channels, 3895, Tocris Bioscience

10 Citations for NS 309

Citations are publications that use Tocris products. Selected citations for NS 309 include:

Sarah Y et al (2020) Site-specific opening of the blood-brain barrier by extracellular histones. J Neuroinflammation 17 281 PMID: 32962721

Zhang et al (2015) Molecular overlap in the regulation of SK channels by small molecules and phosphoinositides. Neurochem Int 1 e1500008 PMID: 26366439

Hakim et al (2018) Electrical dynamics of isolated cerebral and skeletal muscle endothelial tubes: Differential roles of G-protein-coupled receptors and K+ channels. Pharmacol Res Perspect 6 e00391 PMID: 29636977

Brant E et al (2021) Brain endothelial cell TRPA1 channels initiate neurovascular coupling. Elife 10 PMID: 33635784


Do you know of a great paper that uses NS 309 from Tocris? Please let us know.

Reviews for NS 309

Average Rating: 5 (Based on 1 Review.)

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NS 309 worked nicely on experimental conditions.
By Anonymous on 10/29/2018
Assay Type: In Vitro
Species: Human

NS 309 is a potent Kca channel activator. I worked with 10,20,30,40,50,80,100 micrograms dissolved in DMSO. The product worked nicely in experimental conditions and would suggest to others researching Kca channel activation.

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