NS 309

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Cat.No. 3895 - NS 309 | C8H4Cl2N2O2 | CAS No. 18711-16-5
Description: Positive modulator of KCa2 and KCa3.1 channels
Chemical Name: 6,7-Dichloro-1H-indole-2,3-dione 3-oxime
Purity: ≥98% (HPLC)
Datasheet
Citations (5)
Reviews (1)
Literature

Biological Activity

Positive modulator of small- and intermediate- conductance Ca2+-activated K+ channels (KCa2 and KCa3.1 channels); increases Ca2+ sensitivity. Displays no activity at BK channels.

Compound Libraries

NS 309 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 231.04
Formula C8H4Cl2N2O2
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 18711-16-5
PubChem ID 11637204
InChI Key CVOUSAVHMDXCKG-UHFFFAOYSA-N
Smiles ClC1=C2C(C(C(N2)=O)=NO)=CC=C1Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 23.1 100

Preparing Stock Solutions

The following data is based on the product molecular weight 231.04. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.33 mL 21.64 mL 43.28 mL
5 mM 0.87 mL 4.33 mL 8.66 mL
10 mM 0.43 mL 2.16 mL 4.33 mL
50 mM 0.09 mL 0.43 mL 0.87 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Grgic et al (2009) Endothelial Ca2+-activated K+ channels in normal and impaired EDHF-dilator responses - relevance to cardiovascular pathologies and drug discovery. Br.J.Pharmacol. 157 509 PMID: 19302590

Strobaek et al (2004) Activation of human IK and SK Ca2+-activated K+ channels by NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime). Biochim.Biophys.Acta 1665 1 PMID: 15471565

Morimura et al (2006) Voltage-dependent Ca2+-channel block by openers of intermediate and small conductance Ca2+-activated K+ channels in urinary bladder smooth muscle cells. J.Pharm.Sci. 100 237


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View Related Products by Product Action

View all Calcium-Activated Potassium (KCa) Channel Activators

Keywords: NS 309, NS 309 supplier, NS309, Ca2+-activated, potassium, K+, KCa, channels, activators, positive, modulators, small, intermediate, conductance, SK, IK, KCa2, KCa3, Ca2+-Activated, Potassium, Channels, 3895, Tocris Bioscience

5 Citations for NS 309

Citations are publications that use Tocris products. Selected citations for NS 309 include:

Wong et al (2014) Sex differences in endothelial function in porcine coronary arteries: a role for H2O2 and gap junctions?. Br J Pharmacol 171 2751 PMID: 24467384

Oliveira et al (2010) Altered expression and function of small-conductance (SK) Ca(2+)-activated K+ channels in pilocarpine-treated epileptic rats. Brain Res 1348 187 PMID: 20553876

Hipólito et al (2015) In vivo activation of the SK channel in the spinal cord reduces the NMDA receptor antagonist dose needed to produce antinociception in an inflammatory pain model. Pain 156 849 PMID: 25734988

D'Alessandro et al (2013) KCa3.1 channels are involved in the infiltrative behavior of glioblastoma in vivo. Cell Death Dis 4 e773 PMID: 23949222

Zhang et al (2015) Molecular overlap in the regulation of SK channels by small molecules and phosphoinositides. Neurochem Int 1 e1500008 PMID: 26366439


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Reviews for NS 309

Average Rating: 5 (Based on 1 Review.)

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NS 309 worked nicely on experimental conditions: Excellent publishable, performed ideally.
By Anonymous on 10/29/2018
Assay Type: In Vitro
Species: Human

NS 309 is a potent Kca channel activator. I worked with 10,20,30,40,50,80,100 micrograms dissolved in DMSO. The product worked nicely in experimental conditions and would suggest to others researching Kca channel activation.

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