NMDA

Pricing Availability Delivery Time Qty
Cat.No. 0114 - NMDA | C5H9NO4 | CAS No. 6384-92-5
Description: Selective NMDA agonist
Chemical Name: N-Methyl-D-aspartic acid
Purity: ≥99% (HPLC)
Datasheet
Citations (71)
Literature

Biological Activity

Prototypic NMDA receptor agonist. Also available as part of the Mixed NMDA Receptor Tocriset™. Caged version also available (Cat. No 2224).

Technical Data

M. Wt 147.13
Formula C5H9NO4
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 6384-92-5
PubChem ID 4376
InChI Key HOKKHZGPKSLGJE-UHFFFAOYSA-N
Smiles O=C(C(NC)CC(=O)O)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
phosphate buffered saline 14.71 100
1eq. NaOH 14.71 100
water 14.71 100

Preparing Stock Solutions

The following data is based on the product molecular weight 147.13. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 6.8 mL 33.98 mL 67.97 mL
5 mM 1.36 mL 6.8 mL 13.59 mL
10 mM 0.68 mL 3.4 mL 6.8 mL
50 mM 0.14 mL 0.68 mL 1.36 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Watkins and Evans (1981) Excitatory amino acid transmitters. Annu.Rev.Pharmacol.Toxicol. 21 165 PMID: 6112965

Watkins (1978) Excitatory amino acids. Kainic acid as a Tool in Neurobiology. Edited by E 37 PMID:


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Keywords: Selective NMDA agonists Glutamate Receptors N-Methyl-D-Aspartate iGluR Ionotropic NMDA Receptors

71 Citations for NMDA

Citations are publications that use Tocris products. Selected citations for NMDA include:

Udagawa et al (2012) Bidirectional control of mRNA translation and synaptic plasticity by the cytoplasmic polyadenylation complex. Mol Cell 47 253 PMID: 22727665

Niwa et al (2012) Gephyrin-independent GABA(A)R mobility and clustering during plasticity. PLoS One 7 e36148 PMID: 22563445

Ali (2011) CB1 modulation of temporally distinct synaptic facilitation among local circuit interneurons mediated by N-type calcium channels in CA1. J Neurophysiol 105 1051 PMID: 21123660

Yang et al (2011) SynGAP moves out of the core of the postsynaptic density upon depolarization. Neuroscience 192 132 PMID: 21736925

Li et al (2011) Soybean-derived Bowman-Birk inhibitor inhibits neurotoxicity of LPS-activated macrophages. J Neuroinflammation 8 15 PMID: 21324129

Fontainhas et al (2011) Microglial morphology and dynamic behavior is regulated by ionotropic glutamatergic and GABAergic neurotransmission. PLoS One 6 e15973 PMID: 21283568

Meissirel et al (2011) VEGF modulates NMDA receptors activity in cerebellar granule cells through Src-family kinases before synapse formation. Proc Natl Acad Sci U S A 108 13782 PMID: 21804034

Wang and Green (2011) Functional role of neurotrophin-3 in synapse regeneration by spiral ganglion neurons on inner hair cells after excitotoxic trauma in vitro. J Neurosci 31 7938 PMID: 21613508

Lussier et al (2011) Activity-dependent ubiquitination of the AMPA receptor subunit GluA2. J Neurosci 31 3077 PMID: 21414928

Mahajan et al (2011) Exposure of neurons to excitotoxic levels of glutamate induces cleavage of the RNA editing enzyme, adenosine deaminase acting on RNA 2, and loss of GLUR2 editing. Neuroscience 189 305 PMID: 21620933

Maier et al (2010) Sustained glutamate receptor activation down-regulates GABAB receptors by shifting the balance from recycling to lysosomal degradation. J Biol Chem 285 35606 PMID: 20826795

Marignier et al (2010) Oligodendrocytes are damaged by neuromyelitis optica immunoglobulin G via astrocyte injury. Brain 133 2578 PMID: 20688809

Baron et al (2010) NR2D-containing NMDA receptors mediate tissue plasminogen activator-promoted neuronal excitotoxicity. Cell Death Differ 17 860 PMID: 19911010

Pansiot et al (2010) Neuroprotective effect of inhaled nitric oxide on excitotoxic-induced brain damage in neonatal rat. PLoS One 5 e10916 PMID: 20532231

Roselli et al (2009) Disassembly of shank and homer synaptic clusters is driven by soluble beta-amyloid(1-40) through divergent NMDAR-dependent signalling pathways. PLoS One 4 e6011 PMID: 19547699

Citri et al (2009) N-methyl-D-aspartate receptor- and metabotropic glutamate receptor-dependent long-term depression are differentially regulated by the ubiquitin-proteasome system. Eur J Neurosci 30 1443 PMID: 19821836

Furness et al (2008) A quantitative assessment of glutamate uptake into hippocampal synaptic terminals and astrocytes: new insights into a neuronal role for excitatory amino acid transporter 2 (EAAT2). Neuroscience 157 80 PMID: 18805467

Iwasato et al (2008) Cortical adenylyl cyclase 1 is required for thalamocortical synapse maturation and aspects of layer IV barrel development. J Neurosci 28 5931 PMID: 18524897

Wayman et al (2008) An activity-regulated microRNA controls dendritic plasticity by down-regulating p250GAP. Proc Natl Acad Sci U S A 105 9093 PMID: 18577589

Davidkova and Carroll (2007) Characterization of the role of microtubule-associated protein 1B in metabotropic glutamate receptor-mediated endocytosis of AMPA receptors in hippocampus. J Neurosci 27 13273 PMID: 18045921

Iida et al (2007) Synaptic scaffolding molecule alpha is a scaffold to mediate N-methyl-D-aspartate receptor-dependent RhoA activation in dendrites. Mol Cell Biol 27 4388 PMID: 17438139

Holman et al (2007) Differential redistribution of native AMPA receptor complexes following LTD induction in acute hippocampal slices. Neuropharmacology 52 92 PMID: 16815481

Sossa et al (2007) AMPAR exocytosis through NO modulation of PICK1. Neuropharmacology 53 92 PMID: 17555774

Ziskin et al (2007) Vesicular release of glutamate from unmyelinated axons in white matter. Nat Neurosci 10 321 PMID: 17293857

Proudfoot et al (2006) Analgesia mediated by the TRPM8 cold receptor in chronic neuropathic pain. Curr Biol 16 1591 PMID: 16920620

Smith et al (2006) cAMP-dependent protein kinase postsynaptic localization regulated by NMDA receptor activation through translocation of an A-kinase anchoring protein scaffold protein. J Neurosci 26 2391 PMID: 16510716

Lesne et al (2005) Akt-dependent expression of NAIP-1 protects neurons against amyloid-{beta} toxicity. J Biol Chem 280 24941 PMID: 15797869

Lea et al (2005) Neuroprotective activity of the mGluR5 antagonists MPEP and MTEP against acute excitotoxicity differs and does not reflect actions at mGluR5 receptors. Br J Pharmacol 145 527 PMID: 15821750

Yang et al (2005) Behavioral stress enhances hippocampal CA1 long-term depression through the blockade of the glutamate uptake. J Neurosci 25 4288 PMID: 15858055

Nakayama et al (2005) Diminished neuronal activity increases neuron-neuron connectivity underlying silent synapse formation and the rapid conversion of silent to functional synapses. J Neurosci 25 4040 PMID: 15843606

Lenz and Avruch (2005) Glutamatergic regulation of the p70S6 kinase in primary mouse neurons. J Biol Chem 280 38121 PMID: 16183639

Srinivasan et al (2004) Cell type-specific interleukin-1beta signaling in the CNS. J Neurosci 24 6482 PMID: 15269258

Morante-Oria et al (2003) Subpallial origin of a population of projecting pioneer neurons during corticogenesis. Proc Natl Acad Sci U S A 100 12468 PMID: 14523241

Casado et al (2000) Presynaptic N-methyl-D-aspartate receptors at the parallel fiber-Purkinje cell synapse. Proc Natl Acad Sci U S A 97 11593 PMID: 11016958

O'Leary et al (2000) Selective mGluR5 antagonists MPEP and SIB-1893 decrease NMDA or glutamate-mediated neuronal toxicity through actions that reflect NMDA receptor antagonism. Br J Pharmacol 131 1429 PMID: 11090117

Sharifi (2017) The major neurotransmitter systems in the basolateral amygdala and the ventral tegmental areamediate morphine-induced memory consolidation impairment. Neuroscience 353 7 PMID: 28412500

Reinhard et al (2016) The calcium sensor Copine-6 regulates spine structural plasticity and learning and memory. Nat Commun 7 11613 PMID: 27194588

Iskandrani et al (2015) Impact of subanesthetic doses of ketamine on AMPA-mediated responses in rats: An in vivo electrophysiological study on monoaminergic and glutamatergic neurons. J Psychopharmacol 29 792 PMID: 25759403

Curcio et al (2015) Brain ischemia downregulates the neuroprotective GDNF-Ret signaling by a calpain-dependent mechanism in cultured hippocampal neurons. Cell Death Dis 6 e1645 PMID: 25675305

Garthwaite et al (2015) Nitric oxide targets oligodendrocytes and promotes their morphological differentiation. Glia 63 383 PMID: 25327839

Rodríguez-Muñoz et al (2015) The ON:OFF switch, σ1R-HINT1 protein, controls GPCR-NMDA receptor cross-regulation: implications in neurological disorders. Oncotarget 6 35458 PMID: 26461475

Eckel et al (2015) Activation of calcineurin underlies altered trafficking of α2 subunit containing GABAA receptors during prolonged epileptiform activity. Neuropharmacology 88 82 PMID: 25245802

Bell et al (2015) Neuronal development is promoted by weakened intrinsic antioxidant defences due to epigenetic repression of Nrf2. Nat Commun 6 7066 PMID: 25967870

Bertrand et al (2015) Conformations of tissue plasminogen activator (tPA) orchestrate neuronal survival by a crosstalk between EGFR and NMDAR. Cell Death Dis 6 e1924 PMID: 26469972

Lee et al (2014) Long-term depression-inducing stimuli promote cleavage of the synaptic adhesion molecule NGL-3 through NMDA receptors, matrix metalloproteinases and presenilin/γ-secretase. Philos Trans R Soc Lond B Biol Sci 369 20130158 PMID: 24298159

Thein et al (2014) CaMKII mediates recruitment and activation of the deubiquitinase CYLD at the postsynaptic density. PLoS One 9 e91312 PMID: 24614225

An et al (2014) Neuritin can normalize neural deficits of Alzheimer's disease. Cell Death Dis 5 e1523 PMID: 25393479

Chen et al (2014) Presynaptic GABAergic inhibition regulated by BDNF contributes to neuropathic pain induction. Nat Commun 5 5331 PMID: 25354791

Thein et al (2014) IKK regulates the deubiquitinase CYLD at the postsynaptic density. Biochem Biophys Res Commun 450 550 PMID: 24928390

Aligny et al (2014) Ketamine alters cortical integration of GABAergic interneurons and induces long-term sex-dependent impairments in transgenic Gad67-GFP mice. Cell Death Dis 5 e1311 PMID: 24991763

Hsu et al (2014) STAT1 negatively regulates spatial memory formation and mediates the memory-impairing effect of Aβ. Neuropsychopharmacology 39 746 PMID: 24081304

Sánchez-Blázquez et al (2014) The calcium-sensitive σ-1 receptor prevents cannabinoids from provoking glutamate NMDA receptor hypofunction: implications in antinociception and psychotic diseases. Int J Neuropsychopharmacol 17 1943 PMID: 24485144

Antoniou et al (2014) PICK1 links Argonaute 2 to endosomes in neuronal dendrites and regulates miRNA activity. EMBO Rep 15 548 PMID: 24723684

Kung et al (2013) Evidence for glutamate as a neuroglial transmitter within sensory ganglia. PLoS One 8 e68312 PMID: 23844184

Pigott et al (2013) On the selectivity of neuronal NOS inhibitors. Br J Pharmacol 168 1255 PMID: 23072468

Vicente-Sánchez et al (2013) HINT1 protein cooperates with cannabinoid 1 receptor to negatively regulate glutamate NMDA receptor activity. Mol Brain 6 42 PMID: 24093505

Tupone et al (2013) Central activation of the A1 adenosine receptor (A1AR) induces a hypothermic, torpor-like state in the rat. J Neurosci 33 14512 PMID: 24005302

Yang et al (2013) Camkii-mediated phosphorylation regulates distributions of Syngap-α1 and -α2 at the postsynaptic density. PLoS One 8 e71795 PMID: 23967245

Selvakumar et al (2013) S-nitrosylation of AMPA receptor GluA1 regulates phosphorylation, single-channel conductance, and endocytosis. Proc Natl Acad Sci U S A 110 1077 PMID: 23277581

Siddoway et al (2013) An essential role for inhibitor-2 regulation of protein phosphatase-1 in synaptic scaling. J Neurosci 33 11206 PMID: 23825423

Pooler et al (2013) Physiological release of endogenous tau is stimulated by neuronal activity. EMBO Rep 14 389 PMID: 23412472

Owaisat et al (2012) In vivo comparison of harmine efficacy against psychostimulants: preferential inhibition of the cocaine response through a glutamatergic mechanism. Neurosci Lett 525 12 PMID: 22877698

Hedegaard et al (2012) Molecular pharmacology of human NMDA receptors. Neurochem Int 61 601 PMID: 22197913

Huang et al (2012) Glutamate may be an efferent transmitter that elicits inhibition in mouse taste buds. PLoS One 7 e30662 PMID: 22292013

Rodríguez-Muñoz et al (2012) The mu-opioid receptor and the NMDA receptor associate in PAG neurons: implications in pain control. Neuropsychopharmacology 37 338 PMID: 21814188

Wang et al (2012) Reducing amyloid-related Alzheimer's disease pathogenesis by a small molecule targeting filamin A. J Neurosci 32 9773 PMID: 22815492

Bahia et al (2012) Neuroprotective effects of phenolic antioxidant tBHQ associate with inhibition of FoxO3a nuclear translocation and activity. J Neurochem 123 182 PMID: 22804756

He et al (2012) Genetic deletion of TNF receptor suppresses excitatory synaptic transmission via reducing AMPA receptor synaptic localization in cortical neurons. FASEB J 26 334 PMID: 21982949

Tallarida et al (2012) Glutamate carboxypeptidase II (GCPII) inhibitor displays anti-glutamate and anti-cocaine effects in an invertebrate assay. Amino Acids 42 2521 PMID: 21850438

Cunningham et al (2012) Glissandi: transient fast electrocorticographic oscillations of steadily increasing frequency, explained by temporally increasing gap junction conductance. Epilepsia 53 1205 PMID: 22686654

Cassé et al (2012) Glutamate controls tPA recycling by astrocytes, which in turn influences glutamatergic signals. J Neurosci 32 5186 PMID: 22496564


Do you know of a great paper that uses NMDA from Tocris? If so please let us know.

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