NMDA (Cat.No. 0114) caged with the photosensitive 4-methoxy-7-nitroindolinyl group. Stoichiometrically releases chirally pure NMDA. Suitable for uncaging with 300-380 nm excitation. Improved localization can be achieved by uncaging at 405 nm (with mM concentration of caged compound in the bath solution). Exhibits rapid uncaging rate relative to ionotropic glutamate receptor activation.
Sold under license from the UK Medical Research Council
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|water||1.62||5mM with gentle warming|
Preparing Stock Solutions
The following data is based on the product molecular weight 323.3. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.09 mL||15.47 mL||30.93 mL|
|5 mM||0.62 mL||3.09 mL||6.19 mL|
|10 mM||0.31 mL||1.55 mL||3.09 mL|
|50 mM||0.06 mL||0.31 mL||0.62 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Palma-Cerda et al (2012) New caged neurotransmitter analogs selective for glutamate receptor sub-types based on methoxynitroindoline and nitrophenylethoxycarbonyl caging groups. Neuropharmacology. 63 624 PMID: 22609535
If you know of a relevant reference for MNI-caged-NMDA, please let us know.
Keywords: Caged NMDA Compounds Glutamate Receptors N-Methyl-D-Aspartate iGluR Ionotropic MNI-NMDA 4-Methoxy-7-nitroindolinyl-caged N-methyl-D-aspartic acid NMDA Receptors
1 Citation for MNI-caged-NMDA
Citations are publications that use Tocris products. Selected citations for MNI-caged-NMDA include:
Zhang et al (2016) Stereotyped initiation of retinal waves by bipolar cells via presynaptic NMDA autoreceptors. Nat.Commun. 7 12650 PMID: 27586999
Do you know of a great paper that uses MNI-caged-NMDA from Tocris? If so please let us know.
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