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Submit ReviewNG 25
Description: TGF-β-activated kinase (TAK1) inhibitor
Chemical Name: N-[4-[(4-Ethyl-1-piperazinyl)methyl]-3-(trifluoromethyl)phenyl]-4-methyl-3-(1H-pyrrolo[2,3-b]pyridin-4-yloxy)benzamide
Purity: ≥98% (HPLC)
Biological Activity for NG 25
NG 25 is a TGF-β-activated kinase (TAK1) inhibitor (IC50 = 149 nM). Also inhibits LYN, MAP4K2 and Abl kinase activity (IC50 values are 12.9, 21.7 and 75.2 nM, respectively). Suppresses CpG A and CpG B-stimulated secretion of IFN-α and TLR7/8 agonist CL097-stimulated secretion of IFN-β in Gen 2.2 leukemia cells in vitro.
Licensing Information
Sold under license from Dana-Farber Cancer Institute.
Technical Data for NG 25
| M. Wt | 537.58 |
| Formula | C29H30F3N5O2 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1315355-93-1 |
| PubChem ID | 53340664 |
| InChI Key | SMPGEBOIKULBCT-UHFFFAOYSA-N |
| Smiles | CCN1CCN(CC2=C(C(F)(F)F)C=C(NC(C3=CC(OC4=CC=NC5=C4C=CN5)=C(C)C=C3)=O)C=C2)CC1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for NG 25
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 10.75 | 20 |
Preparing Stock Solutions for NG 25
The following data is based on the product molecular weight 537.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 9.3 mL | 46.5 mL | 93.01 mL |
| 1 mM | 1.86 mL | 9.3 mL | 18.6 mL |
| 2 mM | 0.93 mL | 4.65 mL | 9.3 mL |
| 10 mM | 0.19 mL | 0.93 mL | 1.86 mL |
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Product Datasheets for NG 25
References for NG 25
References are publications that support the biological activity of the product.
Tan et al (2015) Discovery of type II inhibitors of TGFβ-activated kinase 1 (TAK1) and mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2). J.Med.Chem. 58 183 PMID: 25075558
Pauls et al (2012) Essential role for IKKβ in production of type 1 IF.s by plasmacytoid dendritic cells. J.Biol.Chem. 287 19216 PMID: 22511786
Dzamko et al (2012) The IkappaB kinase family phosphorylates the Parkinson's disease kinase LRRK2 at Ser935 and Ser910 during Toll-like receptor signaling. PLoS ONE 7 e39132 PMID: 22723946
If you know of a relevant reference for NG 25, please let us know.
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Keywords: NG 25, NG 25 supplier, NG25, TAK1, TGFbeta, TGFb, TGFβ, activated, kinases, inhibitors, inhibits, MAP4K2, LYN, Abl, Other, Kinases, 5429, Tocris Bioscience
Citations for NG 25
Citations are publications that use Tocris products.
Currently there are no citations for NG 25. Do you know of a great paper that uses NG 25 from Tocris? Please let us know.
Reviews for NG 25
Average Rating: 5 (Based on 1 Review.)
Have you used NG 25?
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Works for kinase assays.
By
Scott Scarneo on 02/06/2019
Assay Type: In Vitro
Species: Human
Worked in vitro Kinase assay compared to other TAK1 inhibitors.
