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Cat.No. 5025 - Necrosulfonamide | C18H15N5O6S2 | CAS No. 1360614-48-7
Description: Necroptosis inhibitor
Chemical Name: (2E)-N-[4-[[(3-Methoxy-2-pyrazinyl)amino]sulfonyl]phenyl]-3-(5-nitro-2-thienyl)-2-propenamide
Purity: ≥98% (HPLC)
Citations (2)

Biological Activity

Necroptosis inhibitor. Blocks mixed lineage kinase domain-like protein (MLKL), a critical substrate of receptor-interacting serine-threonine kinase 3 (RIP3) during necrosis. Prevents MLKL-RIP1-RIP3 necrosome complex from interacting with downstream necrosis effectors.

Technical Data

M. Wt 461.47
Formula C18H15N5O6S2
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1360614-48-7
PubChem ID 1566236
Smiles O=C(NC2=CC=C(S(NC3=C(OC)N=CC=N3)(=O)=O)C=C2)/C=C/C1=CC=C([N+]([O-])=O)S1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 9.23 20

Preparing Stock Solutions

The following data is based on the product molecular weight 461.47. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.17 mL 10.83 mL 21.67 mL
5 mM 0.43 mL 2.17 mL 4.33 mL
10 mM 0.22 mL 1.08 mL 2.17 mL
50 mM 0.04 mL 0.22 mL 0.43 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet


References are publications that support the products' biological activity.

Zhou et al (2012) New components of the necroptotic pathway. Protein Cell 3 811 PMID: 23073834

Sun et al (2012) Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148 213 PMID: 22265413

Kreuzaler et al (2012) Killing a cancer: what are the alternatives? Nat.Rev.Cancer. 12 411 PMID: 22576162

If you know of a relevant reference for Necrosulfonamide, please let us know.

View Related Products by Target

Keywords: Necrosulfonamide, supplier, non-apoptotic, cell, death, necroptosis, necrosis, necrosome, mixed, lineage, kinase, domain-like, protein, MLKL, receptor, interacting, serine, threonine, kinases, 3, RIP3, Other, Apoptosis, Other, Apoptosis, Tocris Bioscience

2 Citations for Necrosulfonamide

Citations are publications that use Tocris products. Selected citations for Necrosulfonamide include:

Bittner et al (2016) Death receptor 3 mediates necroptotic cell death Cellular and Molecular Life Sciences PMID: 27592300

Gonzalez-Juarbe et al (2015) Pore-Forming Toxins Induce Macrophage Necroptosis during Acute Bacterial Pneumonia. Channels (Austin) 11 e1005337 PMID: 26659062

Do you know of a great paper that uses Necrosulfonamide from Tocris? If so please let us know.

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Literature in this Area


Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Programmed Cell Death

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Pathways for Necrosulfonamide


Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.


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