Pricing Availability   Qty
Cat.No. 5025 - Necrosulfonamide | C18H15N5O6S2 | CAS No. 1360614-48-7
Description: Necroptosis inhibitor
Chemical Name: (2E)-N-[4-[[(3-Methoxy-2-pyrazinyl)amino]sulfonyl]phenyl]-3-(5-nitro-2-thienyl)-2-propenamide
Purity: ≥98% (HPLC)
Citations (8)
Literature (1)
Pathways (1)

Biological Activity

Necroptosis inhibitor. Blocks mixed lineage kinase domain-like protein (MLKL), a critical substrate of receptor-interacting serine-threonine kinase 3 (RIP3) during necrosis. Prevents MLKL-RIP1-RIP3 necrosome complex from interacting with downstream necrosis effectors.

Technical Data

M. Wt 461.47
Formula C18H15N5O6S2
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1360614-48-7
PubChem ID 1566236
Smiles O=C(NC2=CC=C(S(NC3=C(OC)N=CC=N3)(=O)=O)C=C2)/C=C/C1=CC=C([N+]([O-])=O)S1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 9.23 20

Preparing Stock Solutions

The following data is based on the product molecular weight 461.47. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 10.83 mL 54.17 mL 108.35 mL
1 mM 2.17 mL 10.83 mL 21.67 mL
2 mM 1.08 mL 5.42 mL 10.83 mL
10 mM 0.22 mL 1.08 mL 2.17 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:


References are publications that support the biological activity of the product.

Zhou et al (2012) New components of the necroptotic pathway. Protein Cell 3 811 PMID: 23073834

Sun et al (2012) Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148 213 PMID: 22265413

Kreuzaler et al (2012) Killing a cancer: what are the alternatives? Nat.Rev.Cancer. 12 411 PMID: 22576162

If you know of a relevant reference for Necrosulfonamide, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Necroptosis Inhibitors

Keywords: Necrosulfonamide, Necrosulfonamide supplier, non-apoptotic, cell, death, necroptosis, necrosis, necrosome, mixed, lineage, kinase, domain-like, protein, MLKL, receptor, interacting, serine, threonine, kinases, 3, RIP3, Necroptosis, 5025, Tocris Bioscience

8 Citations for Necrosulfonamide

Citations are publications that use Tocris products. Selected citations for Necrosulfonamide include:

Bagnjuk et al (2019) Necroptosis in primate luteolysis: a role for ceramide. Cell Death Discov 5 67 PMID: 30774995

Chefetz et al (2019) A Pan-ALDH1A Inhibitor Induces Necroptosis in Ovarian Cancer Stem-like Cells. Cell Rep 26 3061 PMID: 30865894

Gonzalez-Juarbe et al (2017) Pore-forming toxin-mediated ion dysregulation leads to death receptor-independent necroptosis of lung epithelial cells during bacterial pneumonia. Cell Death Differ 24 917 PMID: 28387756

Xie et al (2017) The Tumor Suppressor p53 Limits Ferroptosis by Blocking DPP4 Activity. Cell Rep 20 1692 PMID: 28813679

Greer et al (2018) ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget 9 18454 PMID: 29719618

Heulot et al (2016) The TAT-RasGAP317-326 anti-cancer peptide can kill in a caspase-, apoptosis-, and necroptosis-independent manner. Oncotarget 7 64342 PMID: 27602963

Bittner et al (2016) Death receptor 3 mediates necroptotic cell death. Cellular and Molecular Life Sciences PMID: 27592300

Gonzalez-Juarbe et al (2015) Pore-Forming Toxins Induce Macrophage Necroptosis during Acute Bacterial Pneumonia. Channels (Austin) 11 e1005337 PMID: 26659062

Do you know of a great paper that uses Necrosulfonamide from Tocris? Please let us know.

Reviews for Necrosulfonamide

There are currently no reviews for this product. Be the first to review Necrosulfonamide and earn rewards!

Have you used Necrosulfonamide?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Immunology Product Listing

A collection of over 190 products for immunology research, the guide includes research tools for the study of:

  • Chemokine and Cytokine Signaling
  • Chemotaxis
  • Complement System
  • Immune Cell Signaling
  • Inflammation

Pathways for Necrosulfonamide

Toll-like Receptor

Toll-like Receptor Signaling Pathway

TLR signaling is involved in the cellular response to threatening molecules such as bacteria and viruses. It results in an inflammatory and immmunological response.