NADA-green

Pricing Availability   Qty
Cat.No. 6648 - NADA-green | C9H9N5O5.HCl | CAS No. 2253733-11-6
Description: Fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name: 3-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-alanine hydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations (2)
Reviews
Literature (1)

Biological Activity for NADA-green

NADA-green is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Results in strong peripheral and septal labeling of taxonomically diverse bacterial cell populations without affecting growth rate. Excitation/emission λ ~450/555 nm.

please note that the spectral data is solvent dependant

Optical Data for NADA-green

NADA-green Dye Spectra
Emission Color Green
λabs 450 nm
λem 555 nm
Extinction Coefficient (ε) 25,000 M-1cm-1

Technical Data for NADA-green

M. Wt 303.66
Formula C9H9N5O5.HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2253733-11-6
PubChem ID 137919868
InChI Key NKFVSANWNYYLLB-PGMHMLKASA-N
Smiles N[C@@H](C(O)=O)CNC1=CC=C([N+]([O-])=O)C2=NON=C12.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NADA-green

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 30.37 100

Preparing Stock Solutions for NADA-green

The following data is based on the product molecular weight 303.66. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.29 mL 16.47 mL 32.93 mL
5 mM 0.66 mL 3.29 mL 6.59 mL
10 mM 0.33 mL 1.65 mL 3.29 mL
50 mM 0.07 mL 0.33 mL 0.66 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for NADA-green

References are publications that support the biological activity of the product.

Kuru et al (2012) In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew.Chem.Int.Ed. 51 12519 PMID: 23055266

Kuru et al (2014) Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat.Protoc. 10 33 PMID: 25474031

Ladokhin et al (2002) Determining the Membrane Topology of Proteins: Insertion Pathway of a Transmembrane Helix of Annexin 12 Biochemistry 41 13617 PMID: 12427023


If you know of a relevant reference for NADA-green, please let us know.

Keywords: NADA-green, NADA-green supplier, fluorescent, green, D-amino, acid, FDAA, peptidoglycans, labeling, bacteria, labels, labelling, Unnatural, Amino, Acids, Fluorescent, Probes, for, Imaging, Bacteria, 6648, Tocris Bioscience

2 Citations for NADA-green

Citations are publications that use Tocris products. Selected citations for NADA-green include:

Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. ELife 7 PMID: 30324906

Garcia-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. ELife 7 PMID: 30198841


Do you know of a great paper that uses NADA-green from Tocris? Please let us know.

Reviews for NADA-green

There are currently no reviews for this product. Be the first to review NADA-green and earn rewards!

Have you used NADA-green?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals