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Cat.No. 6648 - NADA-green | C9H9N5O5.HCl | CAS No. 2253733-11-6
Description: Fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name: 3-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-alanine hydrochloride
Purity: ≥98% (HPLC)
Citations (2)
Literature (1)

Biological Activity for NADA-green

NADA-green is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Results in strong peripheral and septal labeling of taxonomically diverse bacterial cell populations without affecting growth rate. Excitation/emission λ ~450/555 nm.

please note that the spectral data is solvent dependant

Optical Data for NADA-green

NADA-green Dye Spectra
Emission Color Green
λabs 450 nm
λem 555 nm
Extinction Coefficient (ε) 25,000 M-1cm-1

Technical Data for NADA-green

M. Wt 303.66
Formula C9H9N5O5.HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2253733-11-6
PubChem ID 137919868
Smiles N[C@@H](C(O)=O)CNC1=CC=C([N+]([O-])=O)C2=NON=C12.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NADA-green

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 30.37 100

Preparing Stock Solutions for NADA-green

The following data is based on the product molecular weight 303.66. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.29 mL 16.47 mL 32.93 mL
5 mM 0.66 mL 3.29 mL 6.59 mL
10 mM 0.33 mL 1.65 mL 3.29 mL
50 mM 0.07 mL 0.33 mL 0.66 mL

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References for NADA-green

References are publications that support the biological activity of the product.

Kuru et al (2012) In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew.Chem.Int.Ed. 51 12519 PMID: 23055266

Kuru et al (2014) Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat.Protoc. 10 33 PMID: 25474031

Ladokhin et al (2002) Determining the Membrane Topology of Proteins: Insertion Pathway of a Transmembrane Helix of Annexin 12 Biochemistry 41 13617 PMID: 12427023

If you know of a relevant reference for NADA-green, please let us know.

Keywords: NADA-green, NADA-green supplier, fluorescent, green, D-amino, acid, FDAA, peptidoglycans, labeling, bacteria, labels, labelling, Unnatural, Amino, Acids, Fluorescent, Probes, for, Imaging, Bacteria, 6648, Tocris Bioscience

2 Citations for NADA-green

Citations are publications that use Tocris products. Selected citations for NADA-green include:

Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. ELife 7 PMID: 30324906

Garcia-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. ELife 7 PMID: 30198841

Do you know of a great paper that uses NADA-green from Tocris? Please let us know.

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Literature in this Area

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Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

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