MitoPY1

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Cat.No. 4428 - MitoPY1 | C52H53BIN2O5P | CAS No. 1041634-69-8
Description: Fluorescent mitochondrial hydrogen peroxide indicator
Chemical Name: [4-[4-[3-Oxo-6'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl]-1-piperazinyl]butyl]triphenyl-phosphonium iodide
Datasheet
Citations (1)
Literature

Biological Activity

Fluorescent probe for imaging hydrogen peroxide (H2O2) in mitochondria of living cells. Displays enhanced emission at 528 nm in the presence of H2O2. Selective for H2O2 over superoxide, nitric oxide and hydroxyl radical. Detects local increases in H2O2 in an in vitro model of Parkinson's Disease.

Licensing Information

Sold under license from the Regents of the University of California

Technical Data

M. Wt 954.68
Formula C52H53BIN2O5P
Storage Store at -20°C
CAS Number 1041634-69-8
PubChem ID 90488942
InChI Key JPCCULDYUUMFAD-UHFFFAOYSA-M
Smiles O=C(O4)C5=C(C=CC=C5)C24C1=CC=C(N6CCN(CCCC[P+](C9=CC=CC=C9)(C%10=CC=CC=C%10)C8=CC=CC=C8)CC6)C=C1OC3=C2C=CC(B7OC(C)(C)C(C)(C)O7)=C3.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 95.47 100

Preparing Stock Solutions

The following data is based on the product molecular weight 954.68. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.05 mL 5.24 mL 10.47 mL
5 mM 0.21 mL 1.05 mL 2.09 mL
10 mM 0.1 mL 0.52 mL 1.05 mL
50 mM 0.02 mL 0.1 mL 0.21 mL

Molarity Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Dickinson and Chang (2008) A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells. J.Am.Chem.Soc. 130 9638 PMID: 18605728

Zhao (2009) Lighting up H2O2: the molecule that is a "necessary evil" in the cell. Angew.Chem.Int.Ed.Engl. 48 3022 PMID: 19165849

Guo et al (2013) Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes. J.Biomed.Opt. 18 PMID: 24084856


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Keywords: MitoPY1, supplier, supplier, fluorescent, probes, reagents, imaging, mitochondria, living, cells, hydrogen, peroxide, H2O2, selective, oxidative, stress, ROS, reactive, oxygen, species, Fluorescent, Probes, Fluorescent, Probes, Tocris Bioscience

1 Citation for MitoPY1

Citations are publications that use Tocris products. Selected citations for MitoPY1 include:

Camaré et al (2015) Oxidized LDL-induced angiogenesis involves sphingosine 1-phosphate: prevention by anti-S1P antibody. Br J Pharmacol 172 106 PMID: 25176316


Do you know of a great paper that uses MitoPY1 from Tocris? If so please let us know.

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New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

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