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Description: Fluorescent mitochondrial hydrogen peroxide indicator
Chemical Name: [4-[4-[3-Oxo-6'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl]-1-piperazinyl]butyl]triphenyl-phosphonium iodide
Citations (10)
Reviews (1)
Literature (1)

Biological Activity for MitoPY1

MitoPY1 is a fluorescent probe for imaging hydrogen peroxide (H2O2) in mitochondria of living cells. Displays enhanced emission at 528 nm in the presence of H2O2. Selective for H2O2 over superoxide, nitric oxide and hydroxyl radical. Detects local increases in H2O2 in an in vitro model of Parkinson's Disease.

Licensing Information

Sold under license from the Regents of the University of California

Technical Data for MitoPY1

M. Wt 954.68
Formula C52H53BIN2O5P
Storage Store at -20°C
CAS Number 1041634-69-8
PubChem ID 90488942
Smiles O=C(O4)C5=C(C=CC=C5)C24C1=CC=C(N6CCN(CCCC[P+](C9=CC=CC=C9)(C%10=CC=CC=C%10)C8=CC=CC=C8)CC6)C=C1OC3=C2C=CC(B7OC(C)(C)C(C)(C)O7)=C3.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for MitoPY1

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 95.47 100

Preparing Stock Solutions for MitoPY1

The following data is based on the product molecular weight 954.68. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.05 mL 5.24 mL 10.47 mL
5 mM 0.21 mL 1.05 mL 2.09 mL
10 mM 0.1 mL 0.52 mL 1.05 mL
50 mM 0.02 mL 0.1 mL 0.21 mL

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Product Datasheets for MitoPY1

Certificate of Analysis / Product Datasheet
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References for MitoPY1

References are publications that support the biological activity of the product.

Dickinson and Chang (2008) A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells. J.Am.Chem.Soc. 130 9638 PMID: 18605728

Zhao (2009) Lighting up H2O2: the molecule that is a "necessary evil" in the cell. Angew.Chem.Int.Ed.Engl. 48 3022 PMID: 19165849

Guo et al (2013) Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes. J.Biomed.Opt. 18 PMID: 24084856

If you know of a relevant reference for MitoPY1, please let us know.

Keywords: MitoPY1, MitoPY1 supplier, supplier, fluorescent, probes, reagents, imaging, mitochondria, living, cells, hydrogen, peroxide, H2O2, selective, oxidative, stress, ROS, reactive, oxygen, species, Fluorescent, Cell, Indicators, and, Sensors, Other, Mitochondria, Dyes, Probes, 4428, Tocris Bioscience

10 Citations for MitoPY1

Citations are publications that use Tocris products. Selected citations for MitoPY1 include:

Zlatic et al (2018) Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Cell Syst 6 368 PMID: 29397366

Moreira et al (2019) Prooxidative Activity of Celastrol Induces Apoptosis, DNA Damage, and Cell Cycle Arrest in Drug-Resistant Human Colon Cancer Cells. Oxid Med Cell Longev 2019 6793957 PMID: 31485297

Andrienko et al (2016) Real-time fluorescence measurements of ROS and [Ca2+} in ischemic/reperfused rat hearts: detectable increases occur only after mitochondrial pore opening and are attenuated by ischaemic preconditioning. PLoS One. 11 e0167300 PMID: 27907091

Beyer et al (2014) An acute rise in intraluminal pressure shifts the mediator of flow-mediated dilation from nitric oxide to hydrogen peroxide in human arterioles. Am J Physiol Heart Circ Physiol 307 H1587 PMID: 25260615

Do you know of a great paper that uses MitoPY1 from Tocris? Please let us know.

Reviews for MitoPY1

Average Rating: 4 (Based on 1 Review.)

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Cells were incubated in the well with 8 μg/ml MitoPy diluted in PBS.
By Anonymous on 12/10/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Fibroblasts

Cells were incubated in the well with 8 μg/ml MitoPy diluted in PBS

PMID: 29397366
review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals