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Description: Fluorescent mitochondrial hydrogen peroxide indicator
Chemical Name: [4-[4-[3-Oxo-6'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl]-1-piperazinyl]butyl]triphenyl-phosphonium iodide
Biological Activity for MitoPY1
MitoPY1 is a fluorescent probe for imaging hydrogen peroxide (H2O2) in mitochondria of living cells. Displays enhanced emission at 528 nm in the presence of H2O2. Selective for H2O2 over superoxide, nitric oxide and hydroxyl radical. Detects local increases in H2O2 in an in vitro model of Parkinson's Disease.
Licensing Information
Sold under license from the Regents of the University of California
Technical Data for MitoPY1
| M. Wt | 954.68 |
| Formula | C52H53BIN2O5P |
| Storage | Store at -20°C |
| CAS Number | 1041634-69-8 |
| PubChem ID | 90488942 |
| InChI Key | JPCCULDYUUMFAD-UHFFFAOYSA-M |
| Smiles | O=C(O4)C5=C(C=CC=C5)C24C1=CC=C(N6CCN(CCCC[P+](C9=CC=CC=C9)(C%10=CC=CC=C%10)C8=CC=CC=C8)CC6)C=C1OC3=C2C=CC(B7OC(C)(C)C(C)(C)O7)=C3.[I-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for MitoPY1
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 95.47 | 100 |
Preparing Stock Solutions for MitoPY1
The following data is based on the product molecular weight 954.68. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.05 mL | 5.24 mL | 10.47 mL |
| 5 mM | 0.21 mL | 1.05 mL | 2.09 mL |
| 10 mM | 0.1 mL | 0.52 mL | 1.05 mL |
| 50 mM | 0.02 mL | 0.1 mL | 0.21 mL |
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Product Datasheets for MitoPY1
References for MitoPY1
References are publications that support the biological activity of the product.
Dickinson and Chang (2008) A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells. J.Am.Chem.Soc. 130 9638 PMID: 18605728
Zhao (2009) Lighting up H2O2: the molecule that is a "necessary evil" in the cell. Angew.Chem.Int.Ed.Engl. 48 3022 PMID: 19165849
Guo et al (2013) Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes. J.Biomed.Opt. 18 PMID: 24084856
If you know of a relevant reference for MitoPY1, please let us know.
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Keywords: MitoPY1, MitoPY1 supplier, supplier, fluorescent, probes, reagents, imaging, mitochondria, living, cells, hydrogen, peroxide, H2O2, selective, oxidative, stress, ROS, reactive, oxygen, species, Fluorescent, Cell, Indicators, and, Sensors, Other, Mitochondria, Dyes, Probes, 4428, Tocris Bioscience
10 Citations for MitoPY1
Citations are publications that use Tocris products. Selected citations for MitoPY1 include:
Zlatic et al (2018) Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Cell Syst 6 368 PMID: 29397366
Moreira et al (2019) Prooxidative Activity of Celastrol Induces Apoptosis, DNA Damage, and Cell Cycle Arrest in Drug-Resistant Human Colon Cancer Cells. Oxid Med Cell Longev 2019 6793957 PMID: 31485297
Andrienko et al (2016) Real-time fluorescence measurements of ROS and [Ca2+} in ischemic/reperfused rat hearts: detectable increases occur only after mitochondrial pore opening and are attenuated by ischaemic preconditioning. PLoS One. 11 e0167300 PMID: 27907091
Beyer et al (2014) An acute rise in intraluminal pressure shifts the mediator of flow-mediated dilation from nitric oxide to hydrogen peroxide in human arterioles. Am J Physiol Heart Circ Physiol 307 H1587 PMID: 25260615
Freed et al (2014) Ceramide changes the mediator of flow-induced vasodilation from nitric oxide to hydrogen peroxide in the human microcirculation. Circ Res 115 525 PMID: 24920698
Karunakaran et al (2019) CD36 dependent redoxosomes promotes ceramide-mediated pancreatic β-cell failure via p66Shc activation. Free Radic.Biol.Med. 134 505 PMID: 30735834
Abuaita et al (2018) Mitochondria-Derived Vesicles Deliver Antimicrobial Reactive Oxygen Species to Control Phagosome-Localized Staphylococcus aureus. Cell Host Microbe 24 625 PMID: 30449314
Griffith et al (2015) Metabolic Damage and Premature Thymus Aging Caused by Stromal Catalase Deficiency. Cell Rep 12 1071 PMID: 26257169
Camaré et al (2015) Oxidized LDL-induced angiogenesis involves sphingosine 1-phosphate: prevention by anti-S1P antibody. Br J Pharmacol 172 106 PMID: 25176316
Zhang et al (2018) Mitochondrial redox sensing by the kinase ATM maintains cellular antioxidant capacity. Sci Signal 11 PMID: 29991649
Do you know of a great paper that uses MitoPY1 from Tocris? Please let us know.
Reviews for MitoPY1
Average Rating: 4 (Based on 1 Review.)
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Cells were incubated in the well with 8 μg/ml MitoPy diluted in PBS.
By
Anonymous on 12/10/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Fibroblasts
Cells were incubated in the well with 8 μg/ml MitoPy diluted in PBS
Literature in this Area
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals