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Description: Fluorescent mitochondrial hydrogen peroxide indicator. Used for: live cell imaging. Application: confocal microscopy
Chemical Name: [4-[4-[3-Oxo-6'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl]-1-piperazinyl]butyl]triphenyl-phosphonium iodide
Biological Activity for MitoPY1
Key information: mitochondria peroxy yellow 1 (MitoPY1) is a fluorescent probe for imaging hydrogen peroxide (H2O2) in mitochondria of living cells.
Used for: imaging hydrogen peroxide (H2O2) in mitochondria of living cells. Can also detect local increases in H2O2 in an in vitro model of Parkinson's Disease.
Application: confocal microscopy.
Properties and Photophysical Data: selective for H2O2 over superoxide, nitric oxide and hydroxyl radical.
Excitation and emission maxima (λ) are 510 nm and 530 nm, respectively, quantum yield = 0.405.
Licensing Information
Sold under license from the Regents of the University of California
Optical Data for MitoPY1
| λabs | 510 nm |
|---|---|
| λem | 530 nm |
| Quantum Yield (φ) | 0.405 |
| Application | Confocal Imaging |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for MitoPY1
| M. Wt | 954.68 |
| Formula | C52H53BIN2O5P |
| Storage | Store at -20°C |
| CAS Number | 1041634-69-8 |
| PubChem ID | 90488942 |
| InChI Key | JPCCULDYUUMFAD-UHFFFAOYSA-M |
| Smiles | O=C(O4)C5=C(C=CC=C5)C24C1=CC=C(N6CCN(CCCC[P+](C9=CC=CC=C9)(C%10=CC=CC=C%10)C8=CC=CC=C8)CC6)C=C1OC3=C2C=CC(B7OC(C)(C)C(C)(C)O7)=C3.[I-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for MitoPY1
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 95.47 | 100 |
Preparing Stock Solutions for MitoPY1
The following data is based on the product molecular weight 954.68. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.05 mL | 5.24 mL | 10.47 mL |
| 5 mM | 0.21 mL | 1.05 mL | 2.09 mL |
| 10 mM | 0.1 mL | 0.52 mL | 1.05 mL |
| 50 mM | 0.02 mL | 0.1 mL | 0.21 mL |
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Product Datasheets for MitoPY1
References for MitoPY1
References are publications that support the biological activity of the product.
Dickinson and Chang (2008) A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells. J.Am.Chem.Soc. 130 9638 PMID: 18605728
Zhao (2009) Lighting up H2O2: the molecule that is a "necessary evil" in the cell. Angew.Chem.Int.Ed.Engl. 48 3022 PMID: 19165849
Guo et al (2013) Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes. J.Biomed.Opt. 18 PMID: 24084856
If you know of a relevant reference for MitoPY1, please let us know.
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Keywords: MitoPY1, MitoPY1 supplier, supplier, fluorescent, probes, reagents, imaging, mitochondria, living, cells, hydrogen, peroxide, H2O2, selective, oxidative, stress, ROS, reactive, oxygen, species, Fluorescent, Cell, Indicators, and, Sensors, Other, Mitochondria, Dyes, Probes, 4428, Tocris Bioscience
26 Citations for MitoPY1
Citations are publications that use Tocris products. Selected citations for MitoPY1 include:
João et al (2022) Oxidative and Nitrosative Stress Detection in Human Sperm Using Fluorescent Probes. Methods Mol Biol 2566 45-52 PMID: 36152241
Maria et al (2022) Mitochondrial Alterations in Neurons Derived from the Murine AppNL-F Knock-In Model of Alzheimer's Disease. J Alzheimers Dis 90 565-583 PMID: 36155507
Yangming et al (2022) Redox status regulates autophagy in thymic stromal cells and promotes T cell tolerance. Proc Natl Acad Sci U S A 119 e2204296119 PMID: 36161925
Sun Mi et al (2022) RIP3-Dependent Accumulation of Mitochondrial Superoxide Anions in TNF-α-Induced Necroptosis. Mol Cells 45 193-201 PMID: 35289306
Andreas et al (2022) Persistently Elevated Plasma Concentrations of RIPK3, MLKL, HMGB1, and RIPK1 in Patients with COVID-19 in the Intensive Care Unit. Am J Respir Cell Mol Biol 67 405-408 PMID: 35385375
Chayanon et al (2022) Methods for mitochondrial health assessment by High Content Imaging System. MethodsX 9 101685 PMID: 35464807
J et al (2022) Noncanonical Role of Telomerase in Regulation of Microvascular Redox Environment With Implications for Coronary Artery Disease. Function (Oxf) 3 zqac043 PMID: 36168588
Ines et al (2019) UVB-induced inactivation of manganese-containing superoxide dismutase promotes mitophagy via ROS-mediated mTORC2 pathway activation. J Biol Chem 294 6831-6842 PMID: 30858178
Zhang et al (2018) Mitochondrial redox sensing by the kinase ATM maintains cellular antioxidant capacity. Sci Signal 11 PMID: 29991649
Zlatic et al (2018) Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Cell Syst 6 368 PMID: 29397366
Andrienko et al (2016) Real-time fluorescence measurements of ROS and [Ca2+} in ischemic/reperfused rat hearts: detectable increases occur only after mitochondrial pore opening and are attenuated by ischaemic preconditioning. PLoS One. 11 e0167300 PMID: 27907091
Daria V et al (2020) Renal Glomerular Mitochondria Function in Salt-Sensitive Hypertension. Front Physiol 10 1588 PMID: 32116733
Tong-Shin et al (2020) Diclofenac impairs autophagic flux via oxidative stress and lysosomal dysfunction: Implications for hepatotoxicity. Redox Biol 37 101751 PMID: 33080439
Shu-Hao et al (2020) Different Effects of Metformin and A769662 on Sodium Iodate-Induced Cytotoxicity in Retinal Pigment Epithelial Cells: Distinct Actions on Mitochondrial Fission and Respiration. Antioxidants (Basel) 9 PMID: 33126710
Lucio et al (2020) Measurements of Hydrogen Peroxide and Oxidative DNA Damage in a Cell Model of Premature Aging. Methods Mol Biol 2144 245-257 PMID: 32410041
Abuaita et al (2018) Mitochondria-Derived Vesicles Deliver Antimicrobial Reactive Oxygen Species to Control Phagosome-Localized Staphylococcus aureus. Cell Host Microbe 24 625 PMID: 30449314
Karunakaran et al (2019) CD36 dependent redoxosomes promotes ceramide-mediated pancreatic β-cell failure via p66Shc activation. Free Radic.Biol.Med. 134 505 PMID: 30735834
Caihua et al (2021) Prognostic significance of chemokines CCL11 and CCL5 modulated by low-density lipoprotein cholesterol in colon cancer patients with normal body mass index. Ann Transl Med 9 202 PMID: 33708829
Jorge L et al (2021) Neuronal cell-based high-throughput screen for enhancers of mitochondrial function reveals luteolin as a modulator of mitochondria-endoplasmic reticulum coupling. BMC Biol 19 57 PMID: 33761951
Maria et al (2021) The Sigma-1 Receptor Mediates Pridopidine Rescue of Mitochondrial Function in Huntington Disease Models. Neurotherapeutics 18 1017-1038 PMID: 33797036
Valerie M et al (2021) Adhesion-mediated mechanosignaling forces mitohormesis. Cell Metab 33 1322-1341.e13 PMID: 34019840
Griffith et al (2015) Metabolic Damage and Premature Thymus Aging Caused by Stromal Catalase Deficiency. Cell Rep 12 1071 PMID: 26257169
Camaré et al (2015) Oxidized LDL-induced angiogenesis involves sphingosine 1-phosphate: prevention by anti-S1P antibody. Br J Pharmacol 172 106 PMID: 25176316
Moreira et al (2019) Prooxidative Activity of Celastrol Induces Apoptosis, DNA Damage, and Cell Cycle Arrest in Drug-Resistant Human Colon Cancer Cells. Oxid Med Cell Longev 2019 6793957 PMID: 31485297
Beyer et al (2014) An acute rise in intraluminal pressure shifts the mediator of flow-mediated dilation from nitric oxide to hydrogen peroxide in human arterioles. Am J Physiol Heart Circ Physiol 307 H1587 PMID: 25260615
Freed et al (2014) Ceramide changes the mediator of flow-induced vasodilation from nitric oxide to hydrogen peroxide in the human microcirculation. Circ Res 115 525 PMID: 24920698
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Reviews for MitoPY1
Average Rating: 4 (Based on 1 Review.)
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Cells were incubated in the well with 8 μg/ml MitoPy diluted in PBS.
By
Anonymous on 12/10/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Fibroblasts
Cells were incubated in the well with 8 μg/ml MitoPy diluted in PBS
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Fluorescent Dyes and Probes Research Product GuideUpdated
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