Pricing Availability   Qty
Cat.No. 4428 - MitoPY1 | C52H53BIN2O5P | CAS No. 1041634-69-8
Description: Fluorescent mitochondrial hydrogen peroxide indicator
Chemical Name: [4-[4-[3-Oxo-6'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl]-1-piperazinyl]butyl]triphenyl-phosphonium iodide
Citations (5)

Biological Activity

Fluorescent probe for imaging hydrogen peroxide (H2O2) in mitochondria of living cells. Displays enhanced emission at 528 nm in the presence of H2O2. Selective for H2O2 over superoxide, nitric oxide and hydroxyl radical. Detects local increases in H2O2 in an in vitro model of Parkinson's Disease.

Licensing Information

Sold under license from the Regents of the University of California

Technical Data

M. Wt 954.68
Formula C52H53BIN2O5P
Storage Store at -20°C
CAS Number 1041634-69-8
PubChem ID 90488942
Smiles O=C(O4)C5=C(C=CC=C5)C24C1=CC=C(N6CCN(CCCC[P+](C9=CC=CC=C9)(C%10=CC=CC=C%10)C8=CC=CC=C8)CC6)C=C1OC3=C2C=CC(B7OC(C)(C)C(C)(C)O7)=C3.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 95.47 100

Preparing Stock Solutions

The following data is based on the product molecular weight 954.68. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.05 mL 5.24 mL 10.47 mL
5 mM 0.21 mL 1.05 mL 2.09 mL
10 mM 0.1 mL 0.52 mL 1.05 mL
50 mM 0.02 mL 0.1 mL 0.21 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:


References are publications that support the biological activity of the product.

Dickinson and Chang (2008) A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells. J.Am.Chem.Soc. 130 9638 PMID: 18605728

Zhao (2009) Lighting up H2O2: the molecule that is a "necessary evil" in the cell. Angew.Chem.Int.Ed.Engl. 48 3022 PMID: 19165849

Guo et al (2013) Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes. J.Biomed.Opt. 18 PMID: 24084856

If you know of a relevant reference for MitoPY1, please let us know.

Keywords: MitoPY1, MitoPY1 supplier, supplier, fluorescent, probes, reagents, imaging, mitochondria, living, cells, hydrogen, peroxide, H2O2, selective, oxidative, stress, ROS, reactive, oxygen, species, Fluorescent, Cell, Indicators, and, Sensors, 4428, Tocris Bioscience

5 Citations for MitoPY1

Citations are publications that use Tocris products. Selected citations for MitoPY1 include:

Camaré et al (2015) Oxidized LDL-induced angiogenesis involves sphingosine 1-phosphate: prevention by anti-S1P antibody. Br J Pharmacol 172 106 PMID: 25176316

Griffith et al (2015) Metabolic Damage and Premature Thymus Aging Caused by Stromal Catalase Deficiency. Cell Rep 12 1071 PMID: 26257169

Beyer et al (2014) An acute rise in intraluminal pressure shifts the mediator of flow-mediated dilation from nitric oxide to hydrogen peroxide in human arterioles. Am J Physiol Heart Circ Physiol 307 H1587 PMID: 25260615

Freed et al (2014) Ceramide changes the mediator of flow-induced vasodilation from nitric oxide to hydrogen peroxide in the human microcirculation. Circ Res 115 525 PMID: 24920698

Andrienko et al (2016) Real-time fluorescence measurements of ROS and [Ca2+} in ischemic/reperfused rat hearts: detectable increases occur only after mitochondrial pore opening and are attenuated by ischaemic preconditioning. PLoS One. 11 e0167300 PMID: 27907091

Do you know of a great paper that uses MitoPY1 from Tocris? Please let us know.

Reviews for MitoPY1

There are currently no reviews for this product. Be the first to review MitoPY1 and earn rewards!

Have you used MitoPY1?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

New Product Guide

New Product Guide (Fall/Winter 2018/2019)

Our new product guide highlights over 190 new products added to the Tocris Bioscience range during the second half of 2018.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Chemogenetics
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells