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Cat.No. 4428 - MitoPY1 | C52H53BIN2O5P | CAS No. 1041634-69-8
Description: Fluorescent mitochondrial hydrogen peroxide indicator
Chemical Name: [4-[4-[3-Oxo-6'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl]-1-piperazinyl]butyl]triphenyl-phosphonium iodide
Citations (10)
Reviews (1)
Literature (1)

Biological Activity

Fluorescent probe for imaging hydrogen peroxide (H2O2) in mitochondria of living cells. Displays enhanced emission at 528 nm in the presence of H2O2. Selective for H2O2 over superoxide, nitric oxide and hydroxyl radical. Detects local increases in H2O2 in an in vitro model of Parkinson's Disease.

Licensing Information

Sold under license from the Regents of the University of California

Technical Data

M. Wt 954.68
Formula C52H53BIN2O5P
Storage Store at -20°C
CAS Number 1041634-69-8
PubChem ID 90488942
Smiles O=C(O4)C5=C(C=CC=C5)C24C1=CC=C(N6CCN(CCCC[P+](C9=CC=CC=C9)(C%10=CC=CC=C%10)C8=CC=CC=C8)CC6)C=C1OC3=C2C=CC(B7OC(C)(C)C(C)(C)O7)=C3.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 95.47 100

Preparing Stock Solutions

The following data is based on the product molecular weight 954.68. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

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Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.05 mL 5.24 mL 10.47 mL
5 mM 0.21 mL 1.05 mL 2.09 mL
10 mM 0.1 mL 0.52 mL 1.05 mL
50 mM 0.02 mL 0.1 mL 0.21 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References are publications that support the biological activity of the product.

Dickinson and Chang (2008) A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells. J.Am.Chem.Soc. 130 9638 PMID: 18605728

Zhao (2009) Lighting up H2O2: the molecule that is a "necessary evil" in the cell. Angew.Chem.Int.Ed.Engl. 48 3022 PMID: 19165849

Guo et al (2013) Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes. J.Biomed.Opt. 18 PMID: 24084856

If you know of a relevant reference for MitoPY1, please let us know.

Keywords: MitoPY1, MitoPY1 supplier, supplier, fluorescent, probes, reagents, imaging, mitochondria, living, cells, hydrogen, peroxide, H2O2, selective, oxidative, stress, ROS, reactive, oxygen, species, Fluorescent, Cell, Indicators, and, Sensors, 4428, Tocris Bioscience

10 Citations for MitoPY1

Citations are publications that use Tocris products. Selected citations for MitoPY1 include:

Moreira et al (2019) Prooxidative Activity of Celastrol Induces Apoptosis, DNA Damage, and Cell Cycle Arrest in Drug-Resistant Human Colon Cancer Cells. Oxid Med Cell Longev 2019 6793957 PMID: 31485297

Zhang et al (2018) Mitochondrial redox sensing by the kinase ATM maintains cellular antioxidant capacity. Sci Signal 11 PMID: 29991649

Zlatic et al (2018) Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Cell Syst 6 368 PMID: 29397366

Griffith et al (2015) Metabolic Damage and Premature Thymus Aging Caused by Stromal Catalase Deficiency. Cell Rep 12 1071 PMID: 26257169

Do you know of a great paper that uses MitoPY1 from Tocris? Please let us know.

Reviews for MitoPY1

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Cells were incubated in the well with 8 μg/ml MitoPy diluted in PBS.
By Anonymous on 12/10/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Fibroblasts

Cells were incubated in the well with 8 μg/ml MitoPy diluted in PBS

PMID: 29397366
review image

Literature in this Area

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Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals