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Description: Fluorescent amyloid β detector; brain penetrant
Chemical Name: 4,4'-[(2-methoxy-1,4-phenylene)di-(1E)-2,1-ethenediyl]bisphenol
Purity: ≥98% (HPLC)
Citations (8)
Reviews (1)
Literature (1)

Biological Activity for Methoxy-X04

Methoxy-X04 is a fluorescent amyloid β (Aβ) probe for the detection and quantification of plaques, tangles and cerebrovascular amyloid. Methoxy-X04 displays high in vitro binding affinity (Ki = 26.8 nM); binds selectively to fibrillar β-sheet deposits. Methoxy-X04 is a derivative of Congo Red (Cat. No. 5167). Methoxy-X04 can be used for in vivo Aβ plaque labeling. Methoxy-X04 co-localizes with CD68+ phagosomes in plaque-associated Iba1+ microglia; and it labels molecularly distinct plaque-associated microglia populations. Brain penetrant.

Technical Data for Methoxy-X04

M. Wt 344.4
Formula C23H20O3
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 863918-78-9
PubChem ID 16049314
Smiles OC(C=C3)=CC=C3/C=C/C1=CC(OC)=C(/C=C/C2=CC=C(O)C=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Methoxy-X04

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 34.44 100
ethanol 6.89 20

Preparing Stock Solutions for Methoxy-X04

The following data is based on the product molecular weight 344.4. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.9 mL 14.52 mL 29.04 mL
5 mM 0.58 mL 2.9 mL 5.81 mL
10 mM 0.29 mL 1.45 mL 2.9 mL
50 mM 0.06 mL 0.29 mL 0.58 mL

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Product Datasheets for Methoxy-X04

Certificate of Analysis / Product Datasheet
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References for Methoxy-X04

References are publications that support the biological activity of the product.

Bolmont et al (2008) Dynamics of the microglial/amyloid interaction indicate a role in plaque maintenance. J.Neurosci. 28 4283 PMID: 18417708

Yamanaka et al (2012) PPARγ/RXRα-induced and CD36-mediated microglial amyloid-β phagocytosis results in cognitive improvement in amyloid precursor protein/presenilin 1 mice. J.Neurosci. 32 17321 PMID: 23197723

Klunk et al (2002) Imaging Aβ plaques in living transgenic mice with multiphoton microscopy and methoxy-X04, a systemically administered Congo red derivative. J.Neuropathol.Exp.Neurol. 61 797 PMID: 12230326

Grubman et al (2021) Transcriptional signature in microglia associated with Aβ plaque phagocytosis. Nat.Commun. 12 3015 PMID: 34021136

Heneka et al (2013) NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice. Nature. 493 674 PMID: 23254930

If you know of a relevant reference for Methoxy-X04, please let us know.

Keywords: Methoxy-X04, Methoxy-X04 supplier, amyloid, beta, fluorescent, probes, detection, alzheimers, disease, brain, penetrant, blood, barrier, permeable, high, affinity, amyloidbeta, amyloidb, amyloidβ, Amyloid, Beta, Peptides, Fluorescent, B, Probes, 4920, Tocris Bioscience

8 Citations for Methoxy-X04

Citations are publications that use Tocris products. Selected citations for Methoxy-X04 include:

Fu et al (2016) IL-33 ameliorates Alzheimer's disease-like pathology and cognitive decline. Elife 113 E2705 PMID: 27091974

Socodato et al (2020) Microglia Dysfunction Caused by the Loss of Rhoa Disrupts Neuronal Physiology and Leads to Neurodegeneration Cell Rep 31 PMID: 32579923

Zhao et al (2020) Cellular and Molecular Probing of Intact Human Organs Cell 180 769 PMID: 32059778

Medrano-Jimé nez et al (2019) Malva parviflora extract ameliorates the deleterious effects of a high fat diet on the cognitive deficit in a mouse model of Alzheimer's disease by restoring microglial function via a PPAR-γ-dependent mechanism. J Neuroinflammation 16 143 PMID: 31291963

Do you know of a great paper that uses Methoxy-X04 from Tocris? Please let us know.

Reviews for Methoxy-X04

Average Rating: 5 (Based on 1 Review.)

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Amyloid plaque labelling.
By Anonymous on 11/19/2018
Assay Type: In Vivo
Species: Mouse

Injected IV, Methoxy-X04 labels amyloid plaques in AD models. Great tool to image various cellular processes around AD plaques.

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals