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Description: DNA alkylating agent; cytotoxic and antineoplastic
Chemical Name: 4-[Bis(2-Chloroethyl)amino]-L-phenylalanine
Purity: ≥95% (HPLC)
Citations (2)
Literature (1)
Pathways (1)

Biological Activity for Melphalan

Melphalan is a DNA alkylating agent; induces cytotoxicity through the formation of stable interstrand and intrastrand crosslinks within DNA. Inhibits growth of PC-3 cells (IC50 values are 0.074 and 0.77 μg/ml for sequential dosing and single dosing respectively).

Technical Data for Melphalan

M. Wt 305.2
Formula C13H18Cl2N2O2
Storage Store at RT
Purity ≥95% (HPLC)
CAS Number 148-82-3
PubChem ID 460612
Smiles ClCCN(CCCl)C1=CC=C(C[C@H](N)C(O)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Melphalan

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 6.1 20
1eq. HCl 6.1 20

Preparing Stock Solutions for Melphalan

The following data is based on the product molecular weight 305.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 16.38 mL 81.91 mL 163.83 mL
1 mM 3.28 mL 16.38 mL 32.77 mL
2 mM 1.64 mL 8.19 mL 16.38 mL
10 mM 0.33 mL 1.64 mL 3.28 mL

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Product Datasheets for Melphalan

Certificate of Analysis / Product Datasheet
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References for Melphalan

References are publications that support the biological activity of the product.

Blumberg et al (1965) An alkylating agent for multiple myeloma: melp. JAMA. 191 547 PMID: 14239026

Merck Index 12 5872

Bauer and Povirk (1997) Specificity and kinetics of interstrand and intrastrand bifunctional alkylation by nitrogen mustards at a G-G-C sequence. Nucleic Acids Res. 25 1211 PMID: 9092631

Mougenot et al (2006) In vitro cytotoxic effect of melp. and pilot phase II study in hormone-refractory prostate cancer. Anticancer Res. 26 2197 PMID: 16821586

If you know of a relevant reference for Melphalan, please let us know.

View Related Products by Target

Keywords: Melphalan, Melphalan supplier, antineoplastics, alkylating, DNA, agents, chemotherapeutics, cytotoxic, crosslinks, Other, Apoptosis, 4619, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Melphalan, which is known to the State of California to cause cancer. For more information, go to

2 Citations for Melphalan

Citations are publications that use Tocris products. Selected citations for Melphalan include:

Carlos et al (2019) Chlorambucil targets BRCA1/2-deficient tumours and counteracts PARP inhibitor resistance. EMBO Mol Med 11 e9982 PMID: 31273933

Carlos et al (2019) Chlorambucil targets BRCA1/2-deficient tumours and counteracts PARP inhibitor resistance. EMBO Mol Med PMID: 31127019

Do you know of a great paper that uses Melphalan from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Programmed Cell Death Poster

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Pathways for Melphalan

Apoptosis Signaling Pathway

Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.