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Submit ReviewMelatonin
Description: Endogenous hormone; agonist at MT1 and MT2
Chemical Name: N-Acetyl-5-methoxytryptamine
Purity: ≥99% (HPLC)
Biological Activity for Melatonin
Melatonin is an endogenous hormone that acts as an agonist at melatonin receptors MT1 and MT2. Exhibits a prominent role in the control of circadian rhythm, displays immunomodulatory activity and acts as a powerful antioxidant in vivo.
Compound Libraries for Melatonin
Melatonin is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.
Technical Data for Melatonin
| M. Wt | 232.28 |
| Formula | C13H16N2O2 |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 73-31-4 |
| PubChem ID | 896 |
| InChI Key | DRLFMBDRBRZALE-UHFFFAOYSA-N |
| Smiles | O=C(C)NCCC2=CNC1=CC=C(OC)C=C12 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Melatonin
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.61 | 50 |
Preparing Stock Solutions for Melatonin
The following data is based on the product molecular weight 232.28. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 8.61 mL | 43.05 mL | 86.1 mL |
| 2.5 mM | 1.72 mL | 8.61 mL | 17.22 mL |
| 5 mM | 0.86 mL | 4.31 mL | 8.61 mL |
| 25 mM | 0.17 mL | 0.86 mL | 1.72 mL |
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Product Datasheets for Melatonin
References for Melatonin
References are publications that support the biological activity of the product.
Bubenik (2008) Thirty four years since the discovery of gastrointestinal melatonin. J.Physiol.Pharmacol. 59 33 PMID: 18812627
Fischer et al (2008) Melatonin as a major skin protectant: from free radical scavenging to DNA damage repair. Exp.Dermatol. 17 713 PMID: 18643846
Cardinali et al (2009) Melatonin and melatonergic drugs on sleep: possible mechanisms of action. Int.J.Neurosci. 119 821 PMID: 19326288
If you know of a relevant reference for Melatonin, please let us know.
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Keywords: Melatonin, Melatonin supplier, Endogenous, hormone, agonists, MT1, MT2, Receptors, Melatonin, circadian, rhythms, (MT), 3550, Tocris Bioscience
10 Citations for Melatonin
Citations are publications that use Tocris products. Selected citations for Melatonin include:
Serge et al (2022) PDK4 Inhibition Ameliorates Melatonin Therapy by Modulating Cerebral Metabolism and Remyelination in an EAE Demyelinating Mouse Model of Multiple Sclerosis. Front Immunol 13 862316 PMID: 35355991
Huang et al (2010) Comparative cytotoxicity and intracellular accumulation of five polybrominated diphenyl ether congeners in mouse cerebellar granule neurons. Nature 114 124 PMID: 19969594
Vivek et al (2020) Three-dimensional growth of breast cancer cells potentiates the anti-tumor effects of unacylated ghrelin and AZP-531. Elife 9 PMID: 32667883
Giordano et al (2008) Neurotoxicity of a polybrominated diphenyl ether mixture (DE-71) in mouse neurons and astrocytes is modulated by intracellular glutathione levels. Toxicol Appl Pharmacol 232 161 PMID: 18656495
Chen et al (2014) Antinociceptive effects of novel melatonin receptor agonists in mouse models of abdominal pain. Toxicol Sci 20 1298 PMID: 24574803
Akoume et al (2019) A Differential Hypofunctionality of Gαi Proteins Occurs in Adolescent Idiopathic Scoliosis and Correlates with the Risk of Disease Progression. Sci Rep 9 10074 PMID: 31296888
Hou et al (2017) Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats. Front Cell Neurosci 11 98 PMID: 28473754
Costa et al (2015) The brominated flame retardant BDE-47 causes oxidative stress and apoptotic cell death in vitro and in vivo in mice. Neurotoxicology 48 68 PMID: 25797475
Jie et al (2021) Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats. Front Cell Dev Biol 9 629274 PMID: 33644068
Fernandes et al (2019) Melatonin and Leishmania amazonensis Infection Altered miR-294, miR-30e, and miR-302d Impacting on Tnf, Mcp-1, and Nos2 Expression. Front Cell Infect Microbiol 9 60 PMID: 30949455
Do you know of a great paper that uses Melatonin from Tocris? Please let us know.
Reviews for Melatonin
Average Rating: 5 (Based on 1 Review.)
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Excellent choice for interaction studies with melatonin receptors.
By
Benjamin Stauch on 10/11/2022
Assay Type: In Vitro
Species: Human
This product is an excellent choice for in vitro interaction studies with melatonin receptors. Given that its the endogenous ligand of these receptors, it offers an important point of comparison when testing ligands with unknown activity profiles. In our lab, we typically use this product in micromolar concentrations in all buffers when handling melatonin receptors, unless a more highly stabilizing or potent ligand (such as 2-phenylmelatonin or ramelteon) is needed. These ligands can be more expensive, making melatonin preferable in some cases. We found this product consistently of the highest quality. Highly recommended