LDN 57444

Pricing Availability   Qty
Cat.No. 3998 - LDN 57444 | C17H11Cl3N2O3 | CAS No. 668467-91-2
Description: Ubiquitin C-terminal hydrolase-L1 (UCH-L1) inhibitor
Chemical Name: 5-Chloro-1-[(2,5-dichlorophenyl)methyl]-1H-indole-2,3-dione 3-(O-acetyloxime)
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Reviews (1)
Literature (1)

Biological Activity

Inhibitor of ubiquitin C-terminal hydrolase-L1 (UCH-L1) activity (Ki = 0.4 μM). Causes cell death through the apoptosis pathway; increases levels of highly ubiquitinated proteins and decreases ubiquitin proteasome activity. Activity leads to dramatic alterations in synaptic protein distribution and spine morphology in vivo.

Technical Data

M. Wt 397.64
Formula C17H11Cl3N2O3
Storage Desiccate at +4°C
Purity ≥98% (HPLC)
CAS Number 668467-91-2
PubChem ID 24906255
InChI Key OPQRFPHLZZPCCH-UHFFFAOYSA-N
Smiles ClC1=CC=C(N(CC3=CC(Cl)=CC=C3Cl)C(C2=NOC(C)=O)=O)C2=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 19.88 50

Preparing Stock Solutions

The following data is based on the product molecular weight 397.64. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 5.03 mL 25.15 mL 50.3 mL
2.5 mM 1.01 mL 5.03 mL 10.06 mL
5 mM 0.5 mL 2.51 mL 5.03 mL
25 mM 0.1 mL 0.5 mL 1.01 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:

References

References are publications that support the biological activity of the product.

Liu et al (2003) Discovery of inhibitors that elucidate the role of UCH-L1 activity in the H1299 lung cancer cell line. Chem.Biol. 10 837 PMID: 14522054

Tan et al (2008) Endoplasmic reticulum stress contributes to the cell death induced by UCH-L1 inhibitor. Mol.Cell.Biochem. 318 109 PMID: 18622688

Cartier et al (2009) Regulation of synaptic structure by ubiquitin C-terminal hydrolase L1. J.Neurosci. 29 7857 PMID: 19535597


If you know of a relevant reference for LDN 57444, please let us know.

View Related Products by Product Action

View all Deubiquitinating Enzyme Inhibitors

Keywords: LDN 57444, LDN 57444 supplier, LDN57444, UCH, L1, inhibitors, inhibits, ubiquitin, C-terminal, hydrolases, UCH-L1, Deubiquitinating, Enzymes, 3998, Tocris Bioscience

1 Citation for LDN 57444

Citations are publications that use Tocris products. Selected citations for LDN 57444 include:

Zlatic et al (2018) Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Cell Syst 6 368 PMID: 29397366


Do you know of a great paper that uses LDN 57444 from Tocris? Please let us know.

Reviews for LDN 57444

Average Rating: 4 (Based on 1 Review.)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used LDN 57444?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Culture with LDN57444.
By Anonymous on 12/10/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Fibroblasts

Basal oxygen consumption rates were collected for 4 hours following the addition of CuCl2, LDN57444

PMID: 29397366
review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


New Product Guide

New Product Guide [Spring/Summer 2019]

Our new product guide highlights over 215 new products added to the Tocris Bioscience range during the first half of 2019.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Chemogenetics
  • Epigenetics
  • Fluorescent Imaging
  • PROTACs
  • Signal Transduction
  • Stem Cells