LDN 57444

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Cat.No. 3998 - LDN 57444 | C17H11Cl3N2O3 | CAS No. 668467-91-2
Description: Ubiquitin C-terminal hydrolase-L1 (UCH-L1) inhibitor
Chemical Name: 5-Chloro-1-[(2,5-dichlorophenyl)methyl]-1H-indole-2,3-dione 3-(O-acetyloxime)
Purity: ≥98% (HPLC)
Citations (1)
Reviews (1)

Biological Activity for LDN 57444

LDN 57444 is an inhibitor of ubiquitin C-terminal hydrolase-L1 (UCH-L1) activity (Ki = 0.4 μM). Causes cell death through the apoptosis pathway; increases levels of highly ubiquitinated proteins and decreases ubiquitin proteasome activity. Activity leads to dramatic alterations in synaptic protein distribution and spine morphology in vivo.

Technical Data for LDN 57444

M. Wt 397.64
Formula C17H11Cl3N2O3
Storage Desiccate at +4°C
Purity ≥98% (HPLC)
CAS Number 668467-91-2
PubChem ID 24906255
Smiles ClC1=CC=C(N(CC3=CC(Cl)=CC=C3Cl)C(C2=NOC(C)=O)=O)C2=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for LDN 57444

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 19.88 50

Preparing Stock Solutions for LDN 57444

The following data is based on the product molecular weight 397.64. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 5.03 mL 25.15 mL 50.3 mL
2.5 mM 1.01 mL 5.03 mL 10.06 mL
5 mM 0.5 mL 2.51 mL 5.03 mL
25 mM 0.1 mL 0.5 mL 1.01 mL

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Product Datasheets for LDN 57444

Certificate of Analysis / Product Datasheet
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References for LDN 57444

References are publications that support the biological activity of the product.

Liu et al (2003) Discovery of inhibitors that elucidate the role of UCH-L1 activity in the H1299 lung cancer cell line. Chem.Biol. 10 837 PMID: 14522054

Tan et al (2008) Endoplasmic reticulum stress contributes to the cell death induced by UCH-L1 inhibitor. Mol.Cell.Biochem. 318 109 PMID: 18622688

Cartier et al (2009) Regulation of synaptic structure by ubiquitin C-terminal hydrolase L1. J.Neurosci. 29 7857 PMID: 19535597

If you know of a relevant reference for LDN 57444, please let us know.

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Keywords: LDN 57444, LDN 57444 supplier, LDN57444, UCH, L1, inhibitors, inhibits, ubiquitin, C-terminal, hydrolases, UCH-L1, Deubiquitinating, Enzymes, 3998, Tocris Bioscience

1 Citation for LDN 57444

Citations are publications that use Tocris products. Selected citations for LDN 57444 include:

Zlatic et al (2018) Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Cell Syst 6 368 PMID: 29397366

Do you know of a great paper that uses LDN 57444 from Tocris? Please let us know.

Reviews for LDN 57444

Average Rating: 4 (Based on 1 Review.)

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Culture with LDN57444.
By Anonymous on 12/10/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Fibroblasts

Basal oxygen consumption rates were collected for 4 hours following the addition of CuCl2, LDN57444

PMID: 29397366
review image