Hygromycin B

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Description: Aminoglycoside antibiotic; active against prokaryotic and eukaryotic cells
Chemical Name: O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
Purity: ≥90% (HPLC)
Reviews (1)

Biological Activity for Hygromycin B

Hygromycin B is an aminoglycoside antibiotic. Active against both prokaryotic and eukaryotic cells. Inhibits protein synthesis by binding to the 30S subunit and inhibiting the ribosomal translocation step of elongation.

Technical Data for Hygromycin B

M. Wt 527.52
Formula C20H37N3O13
Storage Store at +4°C
Purity ≥90% (HPLC)
CAS Number 31282-04-9
PubChem ID 90488914
Smiles O[C@H]([C@@H](NC)C[C@@H](N)[C@@H]4O)C4O[C@H]1[C@]2([H])[C@](OC3(O[C@]([H])([C@@H](N)CO)[C@H](O)[C@@H](O)[C@H]3O)O2)([H])[C@@H](O)[C@@H](CO)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Hygromycin B

Solvent Max Conc. mg/mL Max Conc. mM
water 50 95

Preparing Stock Solutions for Hygromycin B

The following data is based on the product molecular weight 527.52. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.95 mM 2 mL 9.98 mL 19.95 mL
4.75 mM 0.4 mL 2 mL 3.99 mL
9.5 mM 0.2 mL 1 mL 2 mL
47.5 mM 0.04 mL 0.2 mL 0.4 mL

Molarity Calculator

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Product Datasheets for Hygromycin B

Certificate of Analysis / Product Datasheet
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References for Hygromycin B

References are publications that support the biological activity of the product.

Blochlinger and Diggelmann (1984) Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eukaryotic cells. Mol.Cell.Biol. 4 2929 PMID: 6098829

Brodersen et al (2000) The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell 103 1143 PMID: 11163189

Ganoza and Kiel (2001) A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob.Agents Chemother. 45 2813 PMID: 11557474

If you know of a relevant reference for Hygromycin B, please let us know.

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Citations for Hygromycin B

Citations are publications that use Tocris products.

Currently there are no citations for Hygromycin B. Do you know of a great paper that uses Hygromycin B from Tocris? Please let us know.

Reviews for Hygromycin B

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Hygromycin used as a selection marker in cells engineered with Hygromycin resistant gene.
By Anonymous on 11/15/2018
Assay Type: In Vitro
Species: Human

Hygromycin was used as a selection marker for few eukaryotic cells which were engineered with Hygromycin resistant gene. Product works beautifully to inhibit translation and killing non resistant strains giving it desirable specificity.

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