Hygromycin B

Pricing Availability Delivery Time Qty
Cat.No. 4137 - Hygromycin B | C20H37N3O13 | CAS No. 31282-04-9
Description: Aminoglycoside antibiotic; active against prokaryotic and eukaryotic cells
Chemical Name: O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
Purity: ≥90% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Aminoglycoside antibiotic. Active against both prokaryotic and eukaryotic cells. Inhibits protein synthesis by binding to the 30S subunit and inhibiting the ribosomal translocation step of elongation.

Technical Data

M. Wt 527.52
Formula C20H37N3O13
Storage Store at +4°C
Purity ≥90% (HPLC)
CAS Number 31282-04-9
PubChem ID 90488914
InChI Key GRRNUXAQVGOGFE-SZNRJEGWSA-N
Smiles O[C@H]([C@@H](NC)C[C@@H](N)[C@@H]4O)C4O[C@H]1[C@]2([H])[C@](OC3(O[C@]([H])([C@@H](N)CO)[C@H](O)[C@@H](O)[C@H]3O)O2)([H])[C@@H](O)[C@@H](CO)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 52.75 100

Preparing Stock Solutions

The following data is based on the product molecular weight 527.52. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.9 mL 9.48 mL 18.96 mL
5 mM 0.38 mL 1.9 mL 3.79 mL
10 mM 0.19 mL 0.95 mL 1.9 mL
50 mM 0.04 mL 0.19 mL 0.38 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Blochlinger and Diggelmann (1984) Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eukaryotic cells. Mol.Cell.Biol. 4 2929 PMID: 6098829

Brodersen et al (2000) The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell 103 1143 PMID: 11163189

Ganoza and Kiel (2001) A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob.Agents Chemother. 45 2813 PMID: 11557474


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Keywords: hygromycinB aminoglycoside antibiotics inhibits protein synthesis ribosomal translocation ribosome 30S eukaryotic prokaryotic cells Antibiotics

Citations for Hygromycin B

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New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for Hygromycin B

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