HLM 006474

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Cat.No. 5283 - HLM 006474 | C25H25N3O2 | CAS No. 353519-63-8
Description: E2F transcription factor inhibitor
Chemical Name: 7-[(4-Ethoxy-3-methylphenyl)(2-pyridinylamino)methyl]-2-methyl-8-quinolinol
Purity: ≥98% (HPLC)

Biological Activity

E2F transcription factor inhibitor; inhibits E2F DNA binding activity. Inhibits E2F4 DNA binding and reduces E2F4 protein levels in A375 melanoma cells. Induces apoptosis of A375 and MDA-MB-231 breast cancer cells. Inhibits A375 proliferation and invasion in a three dimensional skin model of A375 invasion. Also attenuates human embryonic stem cell proliferation.

Technical Data

M. Wt 399.48
Formula C25H25N3O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 353519-63-8
PubChem ID 2895500
Smiles CC1=C(OCC)C=CC(C(C3=C(O)C(N=C(C)C=C4)=C4C=C3)NC2=NC=CC=C2)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 7.99 20

Preparing Stock Solutions

The following data is based on the product molecular weight 399.48. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.5 mL 12.52 mL 25.03 mL
5 mM 0.5 mL 2.5 mL 5.01 mL
10 mM 0.25 mL 1.25 mL 2.5 mL
50 mM 0.05 mL 0.25 mL 0.5 mL

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Product Datasheets

Safety Datasheet


References are publications that support the products' biological activity.

Ma et al (2008) A small-molecule E2F inhibitor blocks growth in a melanoma culture model. Cancer Res. 68 6292 PMID: 18676853

Conklin et al (2012) The RB family is required for the self-renewal and survival of human embryonic stem cells. Nat.Commun. 3 1244 PMID: 23212373

If you know of a relevant reference for HLM 006474, please let us know.

Keywords: HLM 006474, supplier, HLM006474, E2F, transcription, factors, inhibits, inhibitors, DNA, binding, A375, melanoma, stems, cells, proliferation, human, embryonic, Other, Transcription, Factors, Tocris Bioscience

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New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for HLM 006474


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