Home / Cell Biology / Signal Transduction / Transcription Factors / Other Transcription Factors / HLM 006474

HLM 006474

Cat. No. 5283 1 Citation Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Cat.No. 5283 - HLM 006474 | C25H25N3O2 | CAS No. 353519-63-8
Description: E2F transcription factor inhibitor
Chemical Name: 7-[(4-Ethoxy-3-methylphenyl)(2-pyridinylamino)methyl]-2-methyl-8-quinolinol
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Reviews
Literature
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

E2F transcription factor inhibitor; inhibits E2F DNA binding activity. Inhibits E2F4 DNA binding and reduces E2F4 protein levels in A375 melanoma cells. Induces apoptosis of A375 and MDA-MB-231 breast cancer cells. Inhibits A375 proliferation and invasion in a three dimensional skin model of A375 invasion. Also attenuates human embryonic stem cell proliferation.

Technical Data

M. Wt 399.48
Formula C25H25N3O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 353519-63-8
PubChem ID 2895500
InChI Key CYNZBLNMIJNBSF-UHFFFAOYSA-N
Smiles CC1=C(OCC)C=CC(C(C3=C(O)C(N=C(C)C=C4)=C4C=C3)NC2=NC=CC=C2)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.99 20

Preparing Stock Solutions

The following data is based on the product molecular weight 399.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 12.52 mL 62.58 mL 125.16 mL
1 mM 2.5 mL 12.52 mL 25.03 mL
2 mM 1.25 mL 6.26 mL 12.52 mL
10 mM 0.25 mL 1.25 mL 2.5 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References

References are publications that support the biological activity of the product.

Ma et al (2008) A small-molecule E2F inhibitor blocks growth in a melanoma culture model. Cancer Res. 68 6292 PMID: 18676853

Conklin et al (2012) The RB family is required for the self-renewal and survival of human embryonic stem cells. Nat.Commun. 3 1244 PMID: 23212373

If you know of a relevant reference for HLM 006474, please let us know.

View Related Products by Target

Keywords: HLM 006474, HLM 006474 supplier, HLM006474, E2F, transcription, factors, inhibits, inhibitors, DNA, binding, A375, melanoma, stems, cells, proliferation, human, embryonic, Other, Transcription, Factors, Stem, Cell, Proliferation, 5283, Tocris Bioscience

1 Citation for HLM 006474

Citations are publications that use Tocris products. Selected citations for HLM 006474 include:

Dong (2018) BRD4 regulates cellular senescence in gastric cancer cells via E2F/miR-106b/p21 axis. Cell Death Dis 9 203 PMID: 29434197

Do you know of a great paper that uses HLM 006474 from Tocris? Please let us know.

Reviews for HLM 006474

There are currently no reviews for this product. Be the first to review HLM 006474 and earn rewards!

Have you used HLM 006474?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

New Product Guide

New Product Guide (Fall/Winter 2018/2019)

Our new product guide highlights over 190 new products added to the Tocris Bioscience range during the second half of 2018.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Chemogenetics
  • Epigenetics
  • Fluorescent Imaging
  • PROTACs
  • Signal Transduction
  • Stem Cells