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Submit ReviewFNIR-Tag, NHS
Description: Near-infrared fluorescent dye for labeling of amines; supplied as NHS ester
Chemical Name: 2-((E)-2-((E)-2-(3-((4-((2,5-dioxopyrrolidin-1-yl)oxy)-4-oxobutyl)dimethylammonio)propoxy)-3-(2-((E)-1-(2-(2-(2-methoxyethoxy)ethoxy)ethyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)ethylidene)cyclohex-1-en-1-yl)vinyl)-1-(2-(2-(2-methoxyethoxy)ethoxy)ethyl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
Purity: ≥70% (HPLC)
Biological Activity for FNIR-Tag, NHS
FNIR-Tag, NHS is a near-infrared fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. FNIR-Tag, NHS allows a higher degree of labeling (DOL) with less aggregation than leading competitor product. When conjugated to antibodies, FNIR-Tag, NHS exhibits reduced liver uptake in vivo compared with leading competitor conjugates. In addition, FNIR-Tag, NHS conjugates are significantly brighter than leading competitor conjugates at similar labeling densities in vivo and in vitro. Antibody conjugation efficiency is greatest at pH 7.4. Excitation/emission maxima = 766/788 nm; Extinction coefficient = 200,000 M-1cm-1; QY = 0.099.
For further information about how FNIR-Tag, NHS may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Scientific Data
Flow Cytometry
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Application of FNIR-Tag, NHS in vitro. C166 mouse endothelial cells were incubated with 20 nM virus-like particles (VLPs) at 37 °C for 1 h. Samples were extensively washed to remove VLPs not bound to the cellular surface and subsequently fixed to preserve any interactions prior to analysis by flow cytometry. FNIR-Tag conjugates (red) were significantly brighter than IR-800CW conjugates (black) at similar labeling at low (A) and high (B) fluorophore loadings. For more information on this work read Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512
In vivo Imaging
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Application of FNIR-Tag, NHS in vivo. (A) Representative images demonstrating detection of virus-like particle (VLP) conjugates in the liver at 2 h (left panel) and 24 h (right panel) post injection. Scale bar represents epifluorescent in total radiant efficiency. (B) Quantitative analysis of the fluorescence intensity detected at 2 and 24 h post injection. Data are mean value ± standard error. Statistical analysis was performed between groups at the same time point using Student’s t-test. *p ≤ 0.05; **p ≤ 0.01. For more information on this work read Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512
Licensing Information
Sold under license from the National Institute of Health
Optical Data for FNIR-Tag, NHS
| Emission Color | Near-IR |
|---|---|
| Brightness | |
| λabs | 765 nm |
| λem | 788 nm |
| Extinction Coefficient (ε) | 200,000 M-1cm-1 |
| Quantum Yield (φ) | 0.099 |
| Closest Laser line | 750 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Correction Factor 280 | 0.05 |
| Application | Flow Cytometry, In Vivo Imaging |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for FNIR-Tag, NHS
| M. Wt | 1157.4 |
| Formula | C57H80N4O17S2 |
| Storage | Store at -20°C |
| Purity | ≥70% (HPLC) |
| CAS Number | 2365033-54-9 |
| InChI Key | UZSWYHGTBPSLCH-UHFFFAOYSA-N |
| Smiles | [O-]S(=O)(C1=CC=C2[N+](CCOCCOCCOC)=C(C(C)(C2=C1)C)/C=C/C3=C(/C(CCC3)=C/C=C4C(C)(C5=CC(S([O-])(=O)=O)=CC=C5N\4CCOCCOCCOC)C)OCCC[N+](CCCC(ON6C(CCC6=O)=O)=O)(C)C)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for FNIR-Tag, NHS
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.57 | 10 | |
| DMF | 11.57 | 10 |
Preparing Stock Solutions for FNIR-Tag, NHS
The following data is based on the product molecular weight 1157.4. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 8.64 mL | 43.2 mL | 86.4 mL |
| 0.5 mM | 1.73 mL | 8.64 mL | 17.28 mL |
| 1 mM | 0.86 mL | 4.32 mL | 8.64 mL |
| 5 mM | 0.17 mL | 0.86 mL | 1.73 mL |
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Product Datasheets for FNIR-Tag, NHS
References for FNIR-Tag, NHS
References are publications that support the biological activity of the product.
Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512
If you know of a relevant reference for FNIR-Tag, NHS, please let us know.
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Keywords: FNIR-Tag, NHS, FNIR-Tag, NHS supplier, near, infrared, fluorescent, dye, fluorescence, imaging, flow, cytometry, amine, labeling, labelling, NHS, ester, Cyanine, Dyes, (Cy, Dyes), Near, Infrared, (NIR), Fluorescent, 7373, Tocris Bioscience
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Protocols for FNIR-Tag, NHS
The following protocol features additional information for the use of FNIR-Tag, NHS (Cat. No. 7373).
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
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