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Cat.No. 7373 - FNIR-Tag, NHS | C57H80N4O17S2 | CAS No. 2365033-54-9
Description: Near-infrared fluorescent dye for labeling of amines; supplied as NHS ester
Chemical Name: 2-((E)-2-((E)-2-(3-((4-((2,5-dioxopyrrolidin-1-yl)oxy)-4-oxobutyl)dimethylammonio)propoxy)-3-(2-((E)-1-(2-(2-(2-methoxyethoxy)ethoxy)ethyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)ethylidene)cyclohex-1-en-1-yl)vinyl)-1-(2-(2-(2-methoxyethoxy)ethoxy)ethyl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
Purity: ≥90% (HPLC)
Protocols (1)
Literature (1)

Biological Activity for FNIR-Tag, NHS

FNIR-Tag-NHS is a near-infrared fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. FNIR-Tag-NHS allows a higher degree of labeling (DOL) with less aggregation than IRDye® 800CW. When conjugated to antibodies, FNIR-Tag-NHS exhibits reduced liver uptake in vivo compared with IRDye® 800CW conjugates. In addition, FNIR-Tag-NHS conjugates are sigificantly brighter than IRDye® 800CW conjugates at similar labeling densities in vivo and in vitro. Antibody conjugation efficiency is greatest at pH 7.4. Excitation/emission maxima = 766/788 nm; Extinction coefficient = 200,000 M-1cm-1; QY = 0.099.

For further information about how FNIR-Tag, NHS may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.

IRDye® is a registered trademark of LI-COR Biosciences.

Licensing Information

Sold under license from the National Institute of Health

Optical Data for FNIR-Tag, NHS

FNIR-Tag, NHS Dye Spectra
Emission Color Near-IR
λabs 765 nm
λem 788 nm
Extinction Coefficient (ε) 200,000 M-1cm-1
Quantum Yield (φ) 0.099
Closest Laser line 750 nm
Reactive Group NHS ester
Reactivity Primary amines

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Technical Data for FNIR-Tag, NHS

M. Wt 1157.4
Formula C57H80N4O17S2
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 2365033-54-9
Smiles [O-]S(=O)(C1=CC=C2[N+](CCOCCOCCOC)=C(C(C)(C2=C1)C)/C=C/C3=C(/C(CCC3)=C/C=C4C(C)(C5=CC(S([O-])(=O)=O)=CC=C5N\4CCOCCOCCOC)C)OCCC[N+](CCCC(ON6C(CCC6=O)=O)=O)(C)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for FNIR-Tag, NHS

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 11.57 10
DMF 11.57 10

Preparing Stock Solutions for FNIR-Tag, NHS

The following data is based on the product molecular weight 1157.4. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 8.64 mL 43.2 mL 86.4 mL
0.5 mM 1.73 mL 8.64 mL 17.28 mL
1 mM 0.86 mL 4.32 mL 8.64 mL
5 mM 0.17 mL 0.86 mL 1.73 mL

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Product Datasheets for FNIR-Tag, NHS

References for FNIR-Tag, NHS

References are publications that support the biological activity of the product.

Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512

If you know of a relevant reference for FNIR-Tag, NHS, please let us know.

Keywords: FNIR-Tag, NHS, FNIR-Tag, NHS supplier, near, infrared, fluorescent, dye, fluorescence, imaging, flow, cytometry, amine, labeling, labelling, NHS, ester, Cyanine, Dyes, (Cy, Dyes), 7373, Tocris Bioscience

Citations for FNIR-Tag, NHS

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Protocols for FNIR-Tag, NHS

The following protocol features additional information for the use of FNIR-Tag, NHS (Cat. No. 7373).

Literature in this Area

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Fluorescent Probes and Dyes Research Product Guide

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