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Submit ReviewFNIR-Tag, NHS is a near-infrared fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. FNIR-Tag, NHS allows a higher degree of labeling (DOL) with less aggregation than leading competitor product. When conjugated to antibodies, FNIR-Tag, NHS exhibits reduced liver uptake in vivo compared with leading competitor conjugates. In addition, FNIR-Tag, NHS conjugates are significantly brighter than leading competitor conjugates at similar labeling densities in vivo and in vitro. Antibody conjugation efficiency is greatest at pH 7.4. Excitation/emission maxima = 766/788 nm; Extinction coefficient = 200,000 M-1cm-1; QY = 0.099.
For further information about how FNIR-Tag, NHS may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Application of FNIR-Tag, NHS in vitro. C166 mouse endothelial cells were incubated with 20 nM virus-like particles (VLPs) at 37 °C for 1 h. Samples were extensively washed to remove VLPs not bound to the cellular surface and subsequently fixed to preserve any interactions prior to analysis by flow cytometry. FNIR-Tag conjugates (red) were significantly brighter than IR-800CW conjugates (black) at similar labeling at low (A) and high (B) fluorophore loadings. For more information on this work read Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512
Application of FNIR-Tag, NHS in vivo. (A) Representative images demonstrating detection of virus-like particle (VLP) conjugates in the liver at 2 h (left panel) and 24 h (right panel) post injection. Scale bar represents epifluorescent in total radiant efficiency. (B) Quantitative analysis of the fluorescence intensity detected at 2 and 24 h post injection. Data are mean value ± standard error. Statistical analysis was performed between groups at the same time point using Student’s t-test. *p ≤ 0.05; **p ≤ 0.01. For more information on this work read Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512
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Spectral ViewerM. Wt | 1157.4 |
Formula | C57H80N4O17S2 |
Storage | Store at -20°C |
Purity | ≥70% (HPLC) |
CAS Number | 2365033-54-9 |
InChI Key | UZSWYHGTBPSLCH-UHFFFAOYSA-N |
Smiles | [O-]S(=O)(C1=CC=C2[N+](CCOCCOCCOC)=C(C(C)(C2=C1)C)/C=C/C3=C(/C(CCC3)=C/C=C4C(C)(C5=CC(S([O-])(=O)=O)=CC=C5N\4CCOCCOCCOC)C)OCCC[N+](CCCC(ON6C(CCC6=O)=O)=O)(C)C)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solvent | Max Conc. mg/mL | Max Conc. mM | |
---|---|---|---|
Solubility | |||
DMSO | 11.57 | 10 | |
DMF | 11.57 | 10 |
The following data is based on the product molecular weight 1157.4. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
0.1 mM | 8.64 mL | 43.2 mL | 86.4 mL |
0.5 mM | 1.73 mL | 8.64 mL | 17.28 mL |
1 mM | 0.86 mL | 4.32 mL | 8.64 mL |
5 mM | 0.17 mL | 0.86 mL | 1.73 mL |
References are publications that support the biological activity of the product.
Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512
If you know of a relevant reference for FNIR-Tag, NHS, please let us know.
Keywords: FNIR-Tag, NHS, FNIR-Tag, NHS supplier, near, infrared, fluorescent, dye, fluorescence, imaging, flow, cytometry, amine, labeling, labelling, NHS, ester, Cyanine, Dyes, (Cy, Dyes), Near, Infrared, (NIR), Fluorescent, 7373, Tocris Bioscience
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The following protocol features additional information for the use of FNIR-Tag, NHS (Cat. No. 7373).
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
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