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Biological Activity for FNIR-Tag, NHS
FNIR-Tag-NHS is a near-infrared fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. FNIR-Tag-NHS allows a higher degree of labeling (DOL) with less aggregation than IRDye® 800CW. When conjugated to antibodies, FNIR-Tag-NHS exhibits reduced liver uptake in vivo compared with IRDye® 800CW conjugates. In addition, FNIR-Tag-NHS conjugates are sigificantly brighter than IRDye® 800CW conjugates at similar labeling densities in vivo and in vitro. Antibody conjugation efficiency is greatest at pH 7.4. Excitation/emission maxima = 766/788 nm; Extinction coefficient = 200,000 M-1cm-1; QY = 0.099.For further information about how FNIR-Tag, NHS may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.
IRDye® is a registered trademark of LI-COR Biosciences.
Sold under license from the National Institute of Health
Optical Data for FNIR-Tag, NHS
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Technical Data for FNIR-Tag, NHS
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for FNIR-Tag, NHS
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for FNIR-Tag, NHS
The following data is based on the product molecular weight 1157.4. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||8.64 mL||43.2 mL||86.4 mL|
|0.5 mM||1.73 mL||8.64 mL||17.28 mL|
|1 mM||0.86 mL||4.32 mL||8.64 mL|
|5 mM||0.17 mL||0.86 mL||1.73 mL|
References for FNIR-Tag, NHS
References are publications that support the biological activity of the product.
Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512
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Keywords: FNIR-Tag, NHS, FNIR-Tag, NHS supplier, near, infrared, fluorescent, dye, fluorescence, imaging, flow, cytometry, amine, labeling, labelling, NHS, ester, Cyanine, Dyes, (Cy, Dyes), 7373, Tocris Bioscience
Citations for FNIR-Tag, NHS
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Protocols for FNIR-Tag, NHS
The following protocol features additional information for the use of FNIR-Tag, NHS (Cat. No. 7373).
Literature in this Area
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Fluorescent Probes and Dyes Research Product Guide
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