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Biological Activity for dTAG-13
dTAG-13 is a degrader targeting mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. Application of dTAG-13 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo. dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation.
Negative control (Cat. No. 6916) also available.
FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in. Custom knock-in cell lines for the dTAG and aTAG platforms are available from our sister brand R&D Systems. Email TPD@bio-techne.com to enquire.
Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.
Sold under license from Dana-Farber Cancer Institute
Technical Data for dTAG-13
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for dTAG-13
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for dTAG-13
The following data is based on the product molecular weight 1049.18. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.5 mM||1.91 mL||9.53 mL||19.06 mL|
|2.5 mM||0.38 mL||1.91 mL||3.81 mL|
|5 mM||0.19 mL||0.95 mL||1.91 mL|
|25 mM||0.04 mL||0.19 mL||0.38 mL|
References for dTAG-13
References are publications that support the biological activity of the product.
Erb et al (2017) Transcription control by the ENL YEATS domain in acute leukaemia. Nature 543 270 PMID: 28241139
Nabet et al (2018) The dTAG system for immediate and target-specific protein degradation. Nat.Chem.Biol. 14 431 PMID: 29581585
Bensimon et al (2020) Targeted degradation of SLC transporters reveals amenability of multi-pass transmembrane proteins to ligand-induced proteolysis. Cell Chem.Biol. 27 728 PMID: 32386596
If you know of a relevant reference for dTAG-13, please let us know.
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Keywords: dTAG-13, dTAG-13 supplier, dTAG13, degraders, degrades, degradation, FKBP12F36V, fusion, protein, mutant, PROTAC, proteolysis, targeting, chimera, cereblon, PROTACs, TAG, Degradation, Platform, 6605, Tocris Bioscience
20 Citations for dTAG-13
Citations are publications that use Tocris products. Selected citations for dTAG-13 include:
Kristian et al (2021) Generation of locus-specific degradable tag knock-ins in mouse and human cell lines. STAR Protoc 2 100575 PMID: 34151298
Hao et al (2021) Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment. Immunity 54 1392-1404.e10 PMID: 34019797
William P et al (2021) Gene-specific quantification of nascent transcription following targeted degradation of endogenous proteins in cultured cells. STAR Protoc 2 101000 PMID: 34917979
Yuan et al (2022) Effective Menin inhibitor-based combinations against AML with MLL rearrangement or NPM1 mutation (NPM1c). Blood Cancer J 12 5 PMID: 35017466
Daniel A et al (2022) A ubiquitin-independent proteasome pathway controls activation of the CARD8 inflammasome. J Biol Chem 298 102032 PMID: 35580636
Francisca et al (2022) Systematic profiling of conditional degron tag technologies for target validation studies. Nat Commun 13 5495 PMID: 36127368
Rafael et al (2022) BRD2 compartmentalizes the accessible genome. Nat Genet 54 481-491 PMID: 35410381
Fei et al (2022) Regulation of cohesin-mediated chromosome folding by PDS5 in mammals. EMBO Rep 23 e54853 PMID: 36129789
Xin et al (2022) A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Cell Rep 39 110928 PMID: 35675764
Anja et al (2022) NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modes. Nucleic Acids Res 50 5349-5368 PMID: 35489058
Neil et al (2022) Xist-mediated silencing requires additive functions of SPEN and Polycomb together with differentiation-dependent recruitment of SmcHD1. Cell Rep 39 110830 PMID: 35584662
Anna-Katerina et al (2022) Generation of knock-in degron tags for endogenous proteins in mice using the dTAG system. STAR Protoc 3 101660 PMID: 36097386
Tibor et al (2022) The histone methyltransferase SETD2 negatively regulates cell size. J Cell Sci 135 PMID: 36052643
Sahillioglu et al (2021) CRASH-IT switch enables reversible and dose-dependent control of TCR and CAR T-cell function. Cancer Immunol.Res. 9 999 PMID: 34193461
Xavier et al (2019) An efficient auxin-inducible degron system with low basal degradation in human cells. Nat Methods 16 866-869 PMID: 31451765
Kaji et al (2020) Characterization of cereblon-dependent targeted protein degrader by visualizing the spatiotemporal ternary complex formation in cells Sci.Rep. 10 3088 PMID: 32080280
Ma et al (2023) Engineered PROTAC-CID systems for mammalian inducible gene regulation. J Am Chem Soc 145 1593 PMID: 36626587
Orth-He et al (2023) Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation. Cell Rep 42 111965 PMID: 36649711
Rutger A F et al (2023) CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression. Nat Commun 14 3225 PMID: 37270532
Cristian et al (2023) MYC regulates CSF-1 expression via microRNA 17/20a to modulate tumor-associated macrophages in osteosarcoma. JCI Insight PMID: 37279073
Do you know of a great paper that uses dTAG-13 from Tocris? Please let us know.
Reviews for dTAG-13
Average Rating: 5 (Based on 4 Reviews.)
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I tested this product to degrade FFKBP12-Myc protein. It is working nicely.
Assayed the degradation of FKBP-tagged-protein with HA tag in glioma cells using dTAG-13 at 500nM concentration for 0h, 4h, 6h and 24h time intervals. Untransfected cells has no HA signal and in transfected cells with dTAG-13 treatment lead to complete degradation of FKBP-tagged-protein in whole cell lysates
Assayed different dTag concentrations (50nm, 250nm, 500nm) for the knockdown of a FKBP-tagged chromatin-bound protein (180kDa). Whole cell lysate (RIPA) revealed no change to WT cells (150kDa) and considerable knockdown in all conditions after just an hour of treatment.
Used dTAG-13 to degrade a transgene tagged with FKBM12-F36V and HA tags to visualize the protein.
This is a western blot probed for HA tag to track degradation. All samples are treated with 500nM dTAG-13. Lanes are: 1) parental cell line, 2) control (no treatment), 3) 30 mins of treatment, 4) 1hr of treatment, 5) 2hrs of treatment, 6) 4hrs of treatment, 7) 6hrs of treatment. The constructs, as shown by the western, was degraded almost entirely (>98% quantified), within 30 mins. I have tested batch 2 of this product at conc. as low as 50nM and still just as effective. No apparent toxicity to cells. Extremely satisfied.
Literature in this Area
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Targeted Protein Degradation Research Product GuideUpdated
This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:
- Active Degraders
- TAG Degradation Platform
- Degrader Building Blocks
- Ubiquitin-Proteasome System Proteins
- Assays for Protein Degradation
Targeted Protein Degradation Poster
Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia