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dTAG-13

Cat. No. 6605 2 Reviews Submit a Review Datasheet / COA / SDS
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Cat.No. 6605 - dTAG-13 | C57H68N4O15 | CAS No. 2064175-41-1
Description: Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation
Chemical Name: 1-[(2S)-1-Oxo-2-(3,4,5-trimethoxyphenyl)butyl]-(2S)-2-piperidinecarboxylate (1R)-3-(3,4-dimethoxyphenyl)-1-[2-[2-[[6-[[2-(2,6-dioxo-3-piperidinyl)-2,3-dihydro-1,3-dioxo-1H-isoindol-4-yl]oxy]hexyl]amino]-2-oxoethoxy]phenyl]propyl ester
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews (2)
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

PROTAC targeting mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques (via transgene expression or CRISPR-mediated locus-specific knock-in). Application of dTAG-13 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo. dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation.

Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.

Licensing Information

Sold under license from Dana-Farber Cancer Institute

Technical Data

M. Wt 1049.18
Formula C57H68N4O15
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2064175-41-1
PubChem ID 124187630
InChI Key BJFBRLAWLPZOMJ-QHVFGHLPSA-N
Smiles CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NCCCCCCOC4=C5C(N(C(C5=CC=C4)=O)C6CCC(NC6=O)=O)=O)=O)CCC7=CC(OC)=C(C=C7)OC)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 52.46 50
ethanol 20.98 20

Preparing Stock Solutions

The following data is based on the product molecular weight 1049.18. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 1.91 mL 9.53 mL 19.06 mL
2.5 mM 0.38 mL 1.91 mL 3.81 mL
5 mM 0.19 mL 0.95 mL 1.91 mL
25 mM 0.04 mL 0.19 mL 0.38 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the biological activity of the product.

Erb et al (2017) Transcription control by the ENL YEATS domain in acute leukaemia. Nature 543 270 PMID: 28241139

Nabet et al (2018) The dTAG system for immediate and target-specific protein degradation. Nat.Chem.Biol. 14 431 PMID: 29581585

Bensimon et al (2020) Targeted degradation of SLC transporters reveals amenability of multi-pass transmembrane proteins to ligand-induced proteolysis. Cell Chem.Biol. 27 PMID: 32386596

If you know of a relevant reference for dTAG-13, please let us know.

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Keywords: dTAG-13, dTAG-13 supplier, dTAG13, degraders, degrades, degradation, FKBP12F36V, fusion, protein, mutant, PROTAC, proteolysis, targeting, chimera, TAG, Degradation, Platform, 6605, Tocris Bioscience

Citations for dTAG-13

Citations are publications that use Tocris products.

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Reviews for dTAG-13

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Knockdown of FKBP-tagged Chromatin Protein.
By Sam Weeks on 01/30/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: mESC

Assayed different dTag concentrations (50nm, 250nm, 500nm) for the knockdown of a FKBP-tagged chromatin-bound protein (180kDa). Whole cell lysate (RIPA) revealed no change to WT cells (150kDa) and considerable knockdown in all conditions after just an hour of treatment.

review image
Works better than expected.
By Abderhman Abuhashem on 10/14/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: E14 (mouse Embryonic Stem cells)

Used dTAG-13 to degrade a transgene tagged with FKBM12-F36V and HA tags to visualize the protein.

This is a western blot probed for HA tag to track degradation. All samples are treated with 500nM dTAG-13. Lanes are: 1) parental cell line, 2) control (no treatment), 3) 30 mins of treatment, 4) 1hr of treatment, 5) 2hrs of treatment, 6) 4hrs of treatment, 7) 6hrs of treatment. The constructs, as shown by the western, was degraded almost entirely (>98% quantified), within 30 mins. I have tested batch 2 of this product at conc. as low as 50nM and still just as effective. No apparent toxicity to cells. Extremely satisfied.

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Literature in this Area

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Targeted Protein Degradation

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Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia