ClC-Ka chloride channel blocker (IC50 = 100 μM). Blocks the maxi chloride channel in apical membranes from human placenta. Displays antagonistic activity at TRPM4 and TRPC4 channels; potentiates agonist-induced TRPV1 currents (IC50 = 4.88 μM in rat DRG neurons). Inhibits RAD51 recombinase activity (KD = 2 μM).
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|Potassium bicarbonate (0.1M)||4.98||10|
Preparing Stock Solutions
The following data is based on the product molecular weight 498.48. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.01 mL||10.03 mL||20.06 mL|
|5 mM||0.4 mL||2.01 mL||4.01 mL|
|10 mM||0.2 mL||1 mL||2.01 mL|
|50 mM||0.04 mL||0.2 mL||0.4 mL|
References are publications that support the products' biological activity.
Ishida et al (2009) DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange. Nucleic Acids Res. 37 3367 PMID: 19336413
Wulff (2008) New light on the "old" chloride channel blocker DIDS. ACS Chem.Biol. 3 399 PMID: 18642798
Zhang et al (2012) Agonist-dependent potentiation of vanilloid receptor transient receptor potential vanilloid type 1 function by stilbene derivatives. Mol.Pharmacol. 81 689 PMID: 22328719
Riquelme and Parra et al (1999) Regulation of human placental chloride channel by arachidonic acid and other cis unsaturated fatty acids. Am.J.Obstet.Gynecol. 180 469 PMID: 9988821
If you know of a relevant reference for DIDS, please let us know.
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Keywords: DIDS, supplier, chloride, channels, ClC-Ka, blockers, trpm4, trpc4, transient, receptor, potential, vanilloid, receptors, trpv1, rad51, homologous, recombination, dna, damage, repair, double, strand, breaks, 4,4-Diisothiocyanato-2,2-stilbenedisulfonic, Other, Chloride, Channels, Other, Chloride, Channels, Tocris Bioscience
2 Citations for DIDS
Citations are publications that use Tocris products. Selected citations for DIDS include:
Cao et al (2014) SLC17A9 protein functions as a lysosomal ATP transporter and regulates cell viability. BMB Rep 289 23189 PMID: 24962569
Harper and Poole (2013) Chloride channels are necessary for full platelet phosphatidylserine exposure and procoagulant activity. J Biol Chem 4 e969 PMID: 24357800
Do you know of a great paper that uses DIDS from Tocris? If so please let us know.
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