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Description: Chloride channel blocker; TRPV1 modulator; RAD51 recombinase inhibitor
Chemical Name: 4,4'-Diisothiocyanato-2,2'-stilbenedisulfonic acid disodium salt
Purity: ≥95% (HPLC)
Citations (10)
Reviews (1)

Biological Activity for DIDS

DIDS is a ClC-Ka chloride channel blocker (IC50 = 100 μM). Blocks the maxi chloride channel in apical membranes from human placenta. Displays antagonistic activity at TRPM4 and TRPC4 channels; potentiates agonist-induced TRPV1 currents (IC50 = 4.88 μM in rat DRG neurons). Inhibits RAD51 recombinase activity (KD = 2 μM).

Technical Data for DIDS

M. Wt 498.48
Formula C16H8N2Na2O6S4
Storage Store at +4°C
Purity ≥95% (HPLC)
CAS Number 67483-13-0
PubChem ID 5702690
Smiles [Na+].[Na+].[O-]S(=O)(=O)C1=C(\C=C\C2=C(C=C(C=C2)N=C=S)S([O-])(=O)=O)C=CC(=C1)N=C=S

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DIDS

Solvent Max Conc. mg/mL Max Conc. mM
Potassium bicarbonate (0.1M) 2.49 5

Preparing Stock Solutions for DIDS

The following data is based on the product molecular weight 498.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 40.12 mL 200.61 mL 401.22 mL
0.25 mM 8.02 mL 40.12 mL 80.24 mL
0.5 mM 4.01 mL 20.06 mL 40.12 mL
2.5 mM 0.8 mL 4.01 mL 8.02 mL

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Product Datasheets for DIDS

Certificate of Analysis / Product Datasheet
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References for DIDS

References are publications that support the biological activity of the product.

Ishida et al (2009) DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange. Nucleic Acids Res. 37 3367 PMID: 19336413

Wulff (2008) New light on the "old" chloride channel blocker DIDS. ACS Chem.Biol. 3 399 PMID: 18642798

Zhang et al (2012) Agonist-dependent potentiation of vanilloid receptor transient receptor potential vanilloid type 1 function by stilbene derivatives. Mol.Pharmacol. 81 689 PMID: 22328719

Riquelme and Parra et al (1999) Regulation of human placental chloride channel by arachidonic acid and other cis unsaturated fatty acids. Am.J.Obstet.Gynecol. 180 469 PMID: 9988821

If you know of a relevant reference for DIDS, please let us know.

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Keywords: DIDS, DIDS supplier, chloride, channels, ClC-Ka, blockers, trpm4, trpc4, transient, receptor, potential, vanilloid, receptors, trpv1, rad51, homologous, recombination, dna, damage, repair, double, strand, breaks, 4,4-Diisothiocyanato-2,2-stilbenedisulfonic, Other, Chloride, Channels, 4523, Tocris Bioscience

10 Citations for DIDS

Citations are publications that use Tocris products. Selected citations for DIDS include:

Georg et al (2020) Cannabidiol (CBD): a killer for inflammatory rheumatoid arthritis synovial fibroblasts. Cell Death Dis 11 714 PMID: 32873774

Myung-Jin et al (2020) Suppression of CaMKIIβ Inhibits ANO1-Mediated Glioblastoma Progression. Cells 9 PMID: 32357567

Cao et al (2014) SLC17A9 protein functions as a lysosomal ATP transporter and regulates cell viability. BMB Rep 289 23189 PMID: 24962569

Rastogi et al (2021) Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF PLoS Pathog 17 e1009712 PMID: 34324582

Do you know of a great paper that uses DIDS from Tocris? Please let us know.

Reviews for DIDS

Average Rating: 5 (Based on 1 Review.)

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Blocks cellular swelling.
By Anonymous on 02/06/2018
Assay Type: Ex Vivo
Species: Rat

Used at 250microM-500microM to block cellular swelling in the rat brain slice.