DIDS

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Cat.No. 4523 - DIDS | C16H8N2Na2O6S4 | CAS No. 67483-13-0
Description: Chloride channel blocker; TRPV1 modulator; RAD51 recombinase inhibitor
Chemical Name: 4,4'-Diisothiocyanato-2,2'-stilbenedisulfonic acid disodium salt
Purity: ≥95% (HPLC)
Datasheet
Citations (2)
Reviews (1)
Literature (1)

Biological Activity

ClC-Ka chloride channel blocker (IC50 = 100 μM). Blocks the maxi chloride channel in apical membranes from human placenta. Displays antagonistic activity at TRPM4 and TRPC4 channels; potentiates agonist-induced TRPV1 currents (IC50 = 4.88 μM in rat DRG neurons). Inhibits RAD51 recombinase activity (KD = 2 μM).

Technical Data

M. Wt 498.48
Formula C16H8N2Na2O6S4
Storage Store at +4°C
Purity ≥95% (HPLC)
CAS Number 67483-13-0
PubChem ID 5702690
InChI Key GEPAYBXVXXBSKP-SEPHDYHBSA-L
Smiles [Na+].[Na+].[O-]S(=O)(=O)C1=C(\C=C\C2=C(C=C(C=C2)N=C=S)S([O-])(=O)=O)C=CC(=C1)N=C=S

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 4.98 10
Potassium bicarbonate (0.1M) 4.98 10

Preparing Stock Solutions

The following data is based on the product molecular weight 498.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 20.06 mL 100.3 mL 200.61 mL
0.5 mM 4.01 mL 20.06 mL 40.12 mL
1 mM 2.01 mL 10.03 mL 20.06 mL
5 mM 0.4 mL 2.01 mL 4.01 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Ishida et al (2009) DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange. Nucleic Acids Res. 37 3367 PMID: 19336413

Wulff (2008) New light on the "old" chloride channel blocker DIDS. ACS Chem.Biol. 3 399 PMID: 18642798

Zhang et al (2012) Agonist-dependent potentiation of vanilloid receptor transient receptor potential vanilloid type 1 function by stilbene derivatives. Mol.Pharmacol. 81 689 PMID: 22328719

Riquelme and Parra et al (1999) Regulation of human placental chloride channel by arachidonic acid and other cis unsaturated fatty acids. Am.J.Obstet.Gynecol. 180 469 PMID: 9988821


If you know of a relevant reference for DIDS, please let us know.

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Keywords: DIDS, DIDS supplier, chloride, channels, ClC-Ka, blockers, trpm4, trpc4, transient, receptor, potential, vanilloid, receptors, trpv1, rad51, homologous, recombination, dna, damage, repair, double, strand, breaks, 4,4-Diisothiocyanato-2,2-stilbenedisulfonic, Other, Chloride, Channels, 4523, Tocris Bioscience

2 Citations for DIDS

Citations are publications that use Tocris products. Selected citations for DIDS include:

Cao et al (2014) SLC17A9 protein functions as a lysosomal ATP transporter and regulates cell viability. BMB Rep 289 23189 PMID: 24962569

Harper and Poole (2013) Chloride channels are necessary for full platelet phosphatidylserine exposure and procoagulant activity. J Biol Chem 4 e969 PMID: 24357800


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Reviews for DIDS

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Blocks cellular swelling.
By Anonymous on 02/06/2018
Assay Type: Ex Vivo
Species: Rat

Used at 250microM-500microM to block cellular swelling in the rat brain slice.


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