Cytochalasin B

Pricing Availability   Qty

Save up to 40% on RUO Reagents with BIOSPRING24 (see details)

Description: Inhibitor of actin polymerization
Chemical Name: (3E,5R,9R,11E,12aS,13S,15S,15aS,16S,18aS)-6,7,8,9,10,12a,13,14,15,15a,16,17-Dodecahydro-5,13-dihydroxy-9,15-dimethyl-14-methylene-16-(phenylmethyl)-2H-oxacyclotetradecino[2,3-d]isoindole-2,18(5H)-dione
Purity: ≥98% (HPLC)
Citations (7)

Biological Activity for Cytochalasin B

Cytochalasin B is an inhibitor of actin polymerization. Inhibits cell division, migration and glucose transport. Causes G2/M cell cycle arrest and apoptosis in HCT-116 colorectal carcinoma cells. Cell permeable.

Technical Data for Cytochalasin B

M. Wt 479.61
Formula C29H37NO5
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 14930-96-2
PubChem ID 5311281
Smiles C=C([C@H]1C)[C@@H](O)[C@]([C@@]2(O3)[C@]1([H])[C@H](CC4=CC=CC=C4)NC2=O)([H])/C=C/C[C@H](C)CCC[C@@H](O)/C=C/C3=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Cytochalasin B

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 4.8 10 with gentle warming

Preparing Stock Solutions for Cytochalasin B

The following data is based on the product molecular weight 479.61. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 20.85 mL 104.25 mL 208.5 mL
0.5 mM 4.17 mL 20.85 mL 41.7 mL
1 mM 2.09 mL 10.43 mL 20.85 mL
5 mM 0.42 mL 2.09 mL 4.17 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Cytochalasin B

Certificate of Analysis / Product Datasheet
Select another batch:

References for Cytochalasin B

References are publications that support the biological activity of the product.

Theodoropoulos et al (1994) Cytochalasin B may shorten actin filaments by a mechanism independent of barbed end capping. Biochem.Pharmacol. 47 1875 PMID: 8204105

Whitesell et al (2005) Compartmentalization of transport and phosphorylation of glucose in a hepatoma cell line. Biochem.J. 386 245 PMID: 15473866

Buldak et al (2014) Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment. Eur.J.Histochem. 58 2408 PMID: 25308845

If you know of a relevant reference for Cytochalasin B, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Actin Inhibitors

Keywords: Cytochalasin B, Cytochalasin B supplier, inhibitors, inhibits, actin, polymerization, polymerisation, cell, permeable, division, migration, cycle, arrest, cytokinesis, Actin, 5474, Tocris Bioscience

7 Citations for Cytochalasin B

Citations are publications that use Tocris products. Selected citations for Cytochalasin B include:

Maria F et al (2021) ADF and cofilin-1 collaborate to promote cortical actin flow and the leader bleb-based migration of confined cells. Elife 10 PMID: 34169836

Bernier et al (2019) Nanoscale Surveillance of the Brain by Microglia via cAMP-Regulated Filopodia. Cell Rep 27 2895 PMID: 31167136

David Ian et al (2022) Development of reconstructed intestinal micronucleus cytome (RICyt) assay in 3D human gut model for genotoxicity assessment of orally ingested substances. Arch Toxicol 96 1455-1471 PMID: 35226136

Ingrid et al (2022) WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. Dev Cell 57 47-62.e9 PMID: 34919802

Do you know of a great paper that uses Cytochalasin B from Tocris? Please let us know.

Reviews for Cytochalasin B

There are currently no reviews for this product. Be the first to review Cytochalasin B and earn rewards!

Have you used Cytochalasin B?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review