CK 666

Pricing Availability   Qty
Cat.No. 3950 - CK 666 | C18H17FN2O | CAS No. 442633-00-3
Description: Arp2/3 inhibitor; inhibits actin polymerization
Chemical Name: 2-Fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide
Purity: ≥99% (HPLC)
Citations (9)

Biological Activity

Inhibitor of the Arp2/3 complex; inhibits actin polymerization (IC50 = 4 μM).

Technical Data

M. Wt 296.34
Formula C18H17FN2O
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 442633-00-3
PubChem ID 589075
Smiles FC1=CC=CC=C1C(NCCC2=C(C)NC3=C2C=CC=C3)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 29.63 100
ethanol 29.63 100

Preparing Stock Solutions

The following data is based on the product molecular weight 296.34. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.37 mL 16.87 mL 33.75 mL
5 mM 0.67 mL 3.37 mL 6.75 mL
10 mM 0.34 mL 1.69 mL 3.37 mL
50 mM 0.07 mL 0.34 mL 0.67 mL

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References are publications that support the biological activity of the product.

Nolen et al (2009) Characterization of two classes of small molecule inhibitors of Arp2/3 complex. Nature 460 1031 PMID: 19648907

Han and Nolen (2009) X-ray crystal structures and molecular mechanism of improved Arp2/3 complex inhibitors. 1751 ACSB 2009 Abstracts. American Society for Cel

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Keywords: CK 666, CK 666 supplier, CK666, arp2/3, actin, polymerization, inhibitors, inhibits, actin-related, proteins, arp2, arp3, Actin, 3950, Tocris Bioscience

9 Citations for CK 666

Citations are publications that use Tocris products. Selected citations for CK 666 include:

Yamagashi et al (2018) Use of CK-548 and CK-869 as Arp2/3 complex inhibitors directly suppresses microtubule assembly both in vitro and in vivo. Biochem Biophys Res Commun 496 834 PMID: 29395083

Bharadwaj (2017) αV-class integrins exert dual roles on α5β1 integrins to strengthen adhesion to fibronectin. Nat Commun 8 14348 PMID: 28128308

Tornavaca et al (2015) ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation. Proc Natl Acad Sci U S A 208 821 PMID: 25753039

Moreau et al (2015) Signal strength regulates antigen-mediated T-cell deceleration by distinct mechanisms to promote local exploration or arrest. Nat Commun 112 12151 PMID: 26371316

Sorce et al (2015) Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement. Cell Calcium 6 8872 PMID: 26602832

Basu et al (2016) Arp2/3 and VASP Are Essential for Fear Memory Formation in Lateral Amygdala. Eneuro 3 PMID: 27957528

Han et al (2016) Macrophages redirect phagocytosis by non-professional phagocytes and influence inflammation Nature 539 570 PMID: 27820945

Panousopoulou et al (2016) Epiboly generates the epidermal basal monolayer and spreads the nascent mammalian skin to enclose the embryonic body. J Cell Sci 129 1915 PMID: 26989131

Dalton and Carroll (2013) Biased inheritance of mitochondria during asymmetric cell division in the mouse oocyte. J Cell Sci 126 2955 PMID: 23659999

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