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BDY TR-X, SE
Biological Activity for BDY TR-X, SE
BDY TR-X, SE is a fluorescent orange BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to dyes TFAX 594 and Texas Red®. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY TR-X makes it ideal for labeling lipids and cell membranes. BDY TR-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~588 nM; emission maximum ~616 nM.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data for BDY TR-X, SE
|Extinction Coefficient (ε)||68000 M-1cm-1|
|Quantum Yield (φ)||0.9|
|Reactive Group||NHS ester|
|Correction Factor 260||0.15|
|Correction Factor 280||0.19|
|Application||Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy|
|Alternative For||5-TAMRA; Alexa Fluor 555; DyLight 549; TRITC|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
Technical Data for BDY TR-X, SE
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for BDY TR-X, SE
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for BDY TR-X, SE
The following data is based on the product molecular weight 634.46. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.58 mL||7.88 mL||15.76 mL|
|5 mM||0.32 mL||1.58 mL||3.15 mL|
|10 mM||0.16 mL||0.79 mL||1.58 mL|
|50 mM||0.03 mL||0.16 mL||0.32 mL|
References for BDY TR-X, SE
References are publications that support the biological activity of the product.
Vernall et al (2012) Highly potent and selective fluorescent antagonists of the human adenosine A3 receptor based on the 1,2,4-triazolo[4,3-a]quinoxalin-1-one scaffold. J.Med.Chem. 55 1771 PMID: 22277057
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Keywords: BDY TR-X, SE, BDY TR-X, SE supplier, BDYTR-X,SE, 217190131, fluorescent, reagents, labeling, amines, BDY, Dyes, 5467, Tocris Bioscience
Citations for BDY TR-X, SE
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Protocols for BDY TR-X, SE
The following protocol features additional information for the use of BDY TR-X, SE (Cat. No. 5467).
Literature in this Area
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Fluorescent Probes and Dyes Research Product Guide
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