BDY TMR-X, SE

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Cat.No. 5466 - BDY TMR-X, SE | C31H35BF2N4O6 | CAS No. 217190-15-3
Description: Yellow BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester
Chemical Name: (T-4)-[N-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-5-[[5-(4-methoxyphenyl)-2H-pyrrol-2-ylidene-κN]methyl]-2,4-dimethyl-1H-pyrrole-3-propanamidato-κN1]difluoroboron
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Literature (1)

Biological Activity

Fluorescent yellow BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to dye TRITC. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY TMR-X makes it ideal for labeling lipids and cell membranes. BDY TMR-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~544 nM; emission maximum ~570 nM.

BODIPY® is a registered trademark of Molecular Probes.

Optical Data for BDY TMR-X, SE

BDY TMR-X, SE Dye Spectra
Emission Color Yellow
λabs 544 nm
λem 570 nm
Extinction Coefficient (ε) 60000 M-1cm-1
Quantum Yield (φ) 0.96
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.16
Correction Factor 280 0.16
Application Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy
Alternative For 5-TAMRA; Alexa Fluor 555; DyLight 549; TRITC

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M. Wt 608.44
Formula C31H35BF2N4O6
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 217190-15-3
PubChem ID 25164047
InChI Key GRDQURHVJJVDRY-UHFFFAOYSA-N
Smiles CC(C(CCC(NCCCCCC(ON1C(CCC1=O)=O)=O)=O)=C2C)=[N+](C2=C3)[B-](F)(F)N4C3=CC=C4C5=CC=C(OC)C=C5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 60.84 100

Preparing Stock Solutions

The following data is based on the product molecular weight 608.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.64 mL 8.22 mL 16.44 mL
5 mM 0.33 mL 1.64 mL 3.29 mL
10 mM 0.16 mL 0.82 mL 1.64 mL
50 mM 0.03 mL 0.16 mL 0.33 mL

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References

References are publications that support the biological activity of the product.

Clardy et al (2014) Fluorescent exendin-4 derivatives for pancreatic β-cell analysis. Bioconjug.Chem. 25 171 PMID: 24328216


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Keywords: BDY TMR-X, SE, BDY TMR-X, SE supplier, BDYTMR-X,SE, fluorescent, dye, amines, labeling, bodipy, BDY, Dyes, 5466, Tocris Bioscience

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Protocols for BDY TMR-X, SE

The following protocol features additional information for the use of BDY TMR-X, SE (Cat. No. 5466).

Literature in this Area

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