BDY FL Staurosporine

Pricing Availability   Qty
Description: Fluorescent probe targeting kinases; kinase tracer for TR-FRET assays
Purity: ≥95% (HPLC)
Literature (2)

Biological Activity for BDY FL Staurosporine

BDY FL Staurosporine is a fluorescent kinase-targeting probe. It can be paired with antibodies labelled with TR-FRET donors such as CoraFluor™ 1 (Cat. No. 7920) for high throughput binding and protein quantification assays. BDY FL Staurosporine shows high specificity for tyrosine kinase (TK) and tyrosine kinase-like (TKL) family kinases. Excitation and emission maxima (λ) are 514 and 516 nm, respectively; quantum yield = 0.11; extinction coefficient = 35,000 M-1cm-1.

The measured KD values, from TR-FRET based saturation binding assay data, are provided in the accompanying supplementary file (see below).

Supplementary Information for BDY FL Staurosporine

The measured KD values, from TR-FRET based saturation binding assay data, are provided in the accompanying supplementary file.

Optical Data for BDY FL Staurosporine

BDY FL Staurosporine Dye Spectra
Emission Color Green
λabs 514 nm
λem 516 nm
Extinction Coefficient (ε) 35400 M-1cm-1
Quantum Yield (φ) 0.11
Closest Laser line 488 nm
Application Time-resolved fluorescence energy transfer (TR-FRET)

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Technical Data for BDY FL Staurosporine

M. Wt 901.82
Formula C49H54BF2N7O7
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2411845-57-1
PubChem ID 155531579
Smiles C[C@]12N3C4=C(C5=CC=CC=C53)C(CNC6=O)=C6C7=C4N([C@](C[C@H]([C@H]2OC)NCCOCCOCCOCCNC(CCC8=CC=C(C=C9[N+]%10=C(C=C9C)C)N8[B-]%10(F)F)=O)([H])O1)C%11=CC=CC=C7%11

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for BDY FL Staurosporine

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 9.02 10

Preparing Stock Solutions for BDY FL Staurosporine

The following data is based on the product molecular weight 901.82. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 11.09 mL 55.44 mL 110.89 mL
0.5 mM 2.22 mL 11.09 mL 22.18 mL
1 mM 1.11 mL 5.54 mL 11.09 mL
5 mM 0.22 mL 1.11 mL 2.22 mL

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Product Datasheets for BDY FL Staurosporine

References for BDY FL Staurosporine

References are publications that support the biological activity of the product.

Hirozane et al (2019) Structure-based rational design of staurosporine-based fluorescent probe with broad-ranging kinase affinity for kinase panel application. Bioorg.Med.Chem.Lett. 29 126641 PMID: 31526603

If you know of a relevant reference for BDY FL Staurosporine, please let us know.

Keywords: BDY FL Staurosporine, BDY FL Staurosporine supplier, BDY, FL, Staurosporine, fluorescent, probe, BODIPY, fluorescence, probes, assay, reagents, TR-FRET, time-resolved, resonance, energy, transfer, kinases, and, FP, Assay, Reagents, Other, Fluorescent, Probes, 7985, Tocris Bioscience

Citations for BDY FL Staurosporine

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Literature in this Area

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