BDY 650-X, SE

Discontinued Product

BDY 650-X, SE (Cat. No. 5469) has been withdrawn from sale for commercial reasons.
Description: Far-red BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester
Chemical Name: (T-4)-[2-[4-[2-[2-[([2,2'-Bi-1H-pyrrol]-5-yl-κN1)methylene]-2H-pyrrol-5-yl-κN]ethenyl]phenoxy]-N-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]acetamidato]difluoroboron
Purity: ≥90% (HPLC)
Citations (1)
Protocols (1)
Literature (1)

Biological Activity for BDY 650-X, SE

BDY 650-X, SE is a fluorescent far-red BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY 650-X makes it ideal for labeling lipids and cell membranes. BDY 650-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~646 nM; emission maximum ~660 nM.

For more information on how BDY 650-X, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.

BODIPY® is a registered trademark of Molecular Probes.

Optical Data for BDY 650-X, SE

BDY 650-X, SE Dye Spectra
Emission Color Far Red
λabs 646 nm
λem 660 nm
Extinction Coefficient (ε) 102000 M-1cm-1
Quantum Yield (φ) 0.52
Closest Laser line 640 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.04
Correction Factor 280 0.04
Application Confocal fluorescence, fluorescence polarization assays and two-photon excitation (TPE) microscopy
Alternative For BDY 630-X, SE; Cy5; Alexa Fluor 647

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for BDY 650-X, SE

M. Wt 643.44
Formula C33H32BF2N5O6
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 235439-04-0
PubChem ID 25164046
Smiles F[B-]1(F)[N+]2=C(C=CC2=CC3=CC=C(C4=CC=CN4)N13)/C=C/C5=CC=C(OCC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)C=C5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for BDY 650-X, SE

Certificate of Analysis / Product Datasheet
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References for BDY 650-X, SE

References are publications that support the biological activity of the product.

Yang et al (2013) A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking. Nat.Chem. 5 692 PMID: 23881501

Keywords: BDY 650-X, SE, BDY 650-X, SE supplier, BDY650-X,SE, fluorescent, reagents, labeling, amines, bodipy, BDY, Dyes, 5469, Tocris Bioscience

1 Citation for BDY 650-X, SE

Citations are publications that use Tocris products. Selected citations for BDY 650-X, SE include:

Jane et al (2022) NR4A1 modulates intestinal smooth muscle cell phenotype and dampens inflammation-associated intestinal remodeling. FASEB J 36 e22609 PMID: 36250380

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Protocols for BDY 650-X, SE

The following protocol features additional information for the use of BDY 650-X, SE (Cat. No. 5469).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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