BDY 650-X, SE

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Cat.No. 5469 - BDY 650-X, SE | C33H32BF2N5O6 | CAS No. 235439-04-0
Description: Far-red BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester
Chemical Name: (T-4)-[2-[4-[2-[2-[([2,2'-Bi-1H-pyrrol]-5-yl-κN1)methylene]-2H-pyrrol-5-yl-κN]ethenyl]phenoxy]-N-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]acetamidato]difluoroboron
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Literature (1)

Biological Activity

Fluorescent far-red BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY 650-X makes it ideal for labeling lipids and cell membranes. BDY 650-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~646 nM; emission maximum ~660 nM.

This product is predominantly the E isomer with <10% Z isomer present.

BODIPY® is a registered trademark of Molecular Probes.

Optical Data for BDY 650-X, SE

BDY 650-X, SE Dye Spectra
Emission Color Far Red
λabs 646 nm
λem 660 nm
Extinction Coefficient (ε) 102000 M-1cm-1
Quantum Yield (φ) 0.52
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.04
Correction Factor 280 0.04
Application Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy
Alternative For BDY 630-X, SE; Cy5; Alexa Fluor 647

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M. Wt 643.44
Formula C33H32BF2N5O6
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 235439-04-0
PubChem ID 25164046
InChI Key CDXXFTJLAKSQSR-JXMROGBWSA-N
Smiles F[B-]1(F)[N+]2=C(C=CC2=CC3=CC=C(C4=CC=CN4)N13)/C=C/C5=CC=C(OCC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)C=C5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 64.34 100

Preparing Stock Solutions

The following data is based on the product molecular weight 643.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.55 mL 7.77 mL 15.54 mL
5 mM 0.31 mL 1.55 mL 3.11 mL
10 mM 0.16 mL 0.78 mL 1.55 mL
50 mM 0.03 mL 0.16 mL 0.31 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Yang et al (2013) A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking. Nat.Chem. 5 692 PMID: 23881501


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Keywords: BDY 650-X, SE, BDY 650-X, SE supplier, BDY650-X,SE, fluorescent, reagents, labeling, amines, bodipy, BDY, Dyes, 5469, Tocris Bioscience

Citations for BDY 650-X, SE

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Protocols for BDY 650-X, SE

The following protocol features additional information for the use of BDY 650-X, SE (Cat. No. 5469).

Literature in this Area

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