BDY 630-X, SE

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Cat.No. 5468 - BDY 630-X, SE | C33H31BF2N4O6S | CAS No. 380367-48-6
Description: Red BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester
Chemical Name: (T-4)-[2,5-Dioxo-1-pyrrolidinyl 6-[[2-[4-[2-[5-[[5-(2-thienyl)-2H-pyrrol-2-ylidene-κN]methyl]-1H-pyrrol-2-yl-κN]ethenyl]phenoxy]acetyl]amino]hexanoato]difluoroboron
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Literature (1)

Biological Activity

Fluorescent red BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY 630-X makes it ideal for labeling lipid and cell membranes. BDY 630-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~625 nM; emission maximum ~640 nM.

BODIPY® is a registered trademark of Molecular Probes.

Optical Data for BDY 630-X, SE

BDY 630-X, SE Dye Spectra
Emission Color Red
λabs 625 nm
λem 640 nm
Extinction Coefficient (ε) 101000 M-1cm-1
Quantum Yield (φ) 0.91
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.029
Correction Factor 280 0.035
Application Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy
Alternative For BDY 650-X, SE; Cy5; Alexa Fluor 647

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data

M. Wt 660.5
Formula C33H31BF2N4O6S
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 380367-48-6
PubChem ID 25164049
InChI Key TZBGLTNFAMQEEZ-JXMROGBWSA-N
Smiles F[B-]1(F)[N+]2=C(C=CC2=CC3=CC=C(C4=CC=CS4)N13)/C=C/C5=CC=C(OCC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)C=C5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 66.05 100

Preparing Stock Solutions

The following data is based on the product molecular weight 660.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.51 mL 7.57 mL 15.14 mL
5 mM 0.3 mL 1.51 mL 3.03 mL
10 mM 0.15 mL 0.76 mL 1.51 mL
50 mM 0.03 mL 0.15 mL 0.3 mL

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References

References are publications that support the biological activity of the product.

Vernall et al (2012) Highly potent and selective fluorescent antagonists of the human adenosine A3 receptor based on the 1,2,4-triazolo[4,3-a]quinoxalin-1-one scaffold. J.Med.Chem. 55 1771 PMID: 22277057


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Keywords: BDY 630-X, SE, BDY 630-X, SE supplier, BDY630-X,SE, fluorescent, reagents, labeling, amines, bodipy®, BDY, Dyes, 5468, Tocris Bioscience

Citations for BDY 630-X, SE

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Protocols for BDY 630-X, SE

The following protocol features additional information for the use of BDY 630-X, SE (Cat. No. 5468).

Literature in this Area

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