6-Hydroxydopamine hydrobromide

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Cat.No. 2547 - 6-Hydroxydopamine hydrobromide | C8H11NO3.HBr | CAS No. 636-00-0
Description: Selective catecholaminergic neurotoxin
Alternative Names: 6-OHDA
Chemical Name: 5-(2-Aminoethyl)-1,2,4-benzenetriol hydrobromide
Datasheet
Citations (11)
Reviews (2)
Literature (3)

Biological Activity for 6-Hydroxydopamine hydrobromide

6-Hydroxydopamine hydrobromide is a selective catecholaminergic neurotoxin. Depletes brain catecholamine levels via uptake and accumulation by a transport mechanism specific to these neurons. Causes almost complete destruction of nigral dopaminergic neurons and their striatal terminals when injected into the substantia nigra of rats, producing an animal model of Parkinson's disease.

Technical Data for 6-Hydroxydopamine hydrobromide

M. Wt 250.09
Formula C8H11NO3.HBr
Storage Desiccate at -20°C
CAS Number 636-00-0
PubChem ID 176170
InChI Key MLACDGUOKDOLGC-UHFFFAOYSA-N
Smiles NCCC1=CC(O)=C(O)C=C1O.Br

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 6-Hydroxydopamine hydrobromide

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 20.56 100
DMSO 20.56 100

Preparing Stock Solutions for 6-Hydroxydopamine hydrobromide

The following data is based on the product molecular weight 250.09. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4 mL 19.99 mL 39.99 mL
5 mM 0.8 mL 4 mL 8 mL
10 mM 0.4 mL 2 mL 4 mL
50 mM 0.08 mL 0.4 mL 0.8 mL

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Product Datasheets for 6-Hydroxydopamine hydrobromide

Certificate of Analysis / Product Datasheet
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References for 6-Hydroxydopamine hydrobromide

References are publications that support the biological activity of the product.

Breese and Traylor (1970) Effect of 6-hydroxyDA on brain NE and DA: evidence for selective degeneration of catecholamine neurons. J.Pharmacol.Exp.Ther. 174 413 PMID: 5456173

Soto-Otero et al (2000) Autoxidation and neurotoxicity of 6-hydroxyDA in the presence of some antioxidants: potential implication in relation to the pathogenesis of Parkinson's disease. J.Neurochem. 74 1605 PMID: 10737618

Fujita et al (2006) Cell-permeable cAMP analog suppresses 6-hydroxyDA-induced apoptosis in PC12 cells through the activation of the Akt pathway. Brain Res. 1113 10 PMID: 16945353


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Keywords: 6-Hydroxydopamine hydrobromide, 6-Hydroxydopamine hydrobromide supplier, Selective, catecholaminergic, neurotoxin, Dopamine, Receptors, adrenoceptors, adrenergic, dopaminergic, neurotoxins, parkinson's, parkinsons, 6-OHDA, Dopaminergic-Related, Adrenergic, Related, Compounds, 2547, Tocris Bioscience

11 Citations for 6-Hydroxydopamine hydrobromide

Citations are publications that use Tocris products. Selected citations for 6-Hydroxydopamine hydrobromide include:

Kitta et al (2012) Suppression of bladder overactivity by adenosine A2A receptor antagonist in a rat model of Parkinson disease. J Urol 187 1890 PMID: 22425056

Fan (2018) External light activates hair follicle stem cells through eyes via an ipRGC-SCN-sympathetic neural pathway. Proc Natl Acad Sci U S A. 115 E6880 PMID: 29959210

Reinhardt et al (2013) Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling. Neural Plast 8 e59252 PMID: 23533608

McIver et al (2019) Maladaptive Downregulation of Autonomous Subthalamic Nucleus Activity following the Loss of Midbrain Dopamine Neurons Cell Rep 28 992 PMID: 31340159


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Reviews for 6-Hydroxydopamine hydrobromide

Average Rating: 4.5 (Based on 2 Reviews.)

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Works well.
By Anonymous on 06/15/2020
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: neuron

3-4 mg/ml

PMID: 31340159
review image

Great neurotoxic effects in dopaminergic neurons.
By Luiz Alexandre Magno on 07/03/2018
Assay Type: In Vivo
Species: Mouse

I used the 6-Hydroxydopamine (6-OHDA) to deplete dopaminergic cells from the mouse brain, which is largely used as an experimental model of Parkinson's Disease. The 6-OHDA was injected into the mouse striatum and 21 days later we observed that the dopaminergic neurons (positive for tyrosine hydroxylase immunofluorescence) were almost absent in the substantia nigra pars compacta (SNc) from the 6-OHDA injected hemisphere (on the left). As a control for analytical comparisons, we also injected PBS into the contralateral hemisphere (on the right). When this neurotoxin is injected into striatum, as in this case, there is only a slight depletion of dopaminergic neurons from the ventral tegmental area (the region located into the middle of the image). The animals injected with 6-OHDA display several motor dysfunctions and are used to study the pathology and therapies for Parkinsonian symptoms.

Although 6-Hydroxydopamine hydrobromide (6-OHDA.HBr #2547) dissolves very well in aqueous solutions, some tips are necessary to guarantee its stability for biological experiments. 1. When calculating the 6-OHDA concentration, take in consideration that the doses that are often reported are for the free base and not for the hydrobromide (HBr) conjugated salt. Thus, in the case of 6-OHDA.HBr (#2547), the presence of HBr has to be taken into account in making the proper solution. HBr has a molecular weight of 80.91 and represents 32.352% of the 6-OHDA.HBr (#2547) molecular mass.2. Always dissolve the 6-OHDA salt in buffers containing antioxidant capacity. I suggest adding 1 mM ascorbic acid (#4055) into PBS. I know some colleagues that have used sodium metabisulfite as well with great success.3. Always make fresh solutions. The 6-OHDA solution is extremely prone to oxidation, even when resuspended in antioxidant buffers. When fresh, it looks dark red. However, it turns to brown after a few hours.4. Finally, 6-OHDA is extremely toxic. Therefore always be cautious when handling it.

Reference
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