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Biological Activity for 5-Ph-IAA
5-Ph-IAA is a selective and potent "Bump & Hole" TAG Degrader (DC50,6h = 17.0 nM, T1/2 = 62.3 min), for use within an auxin-inducible degron (AID2) system. 5-Ph-IAA is an analog (the “Bump”) of natural auxin that induces rapid degradation of a target protein tagged with mAID F-box protein (the "Hole" or mini AID), and recruits a mutant E3 ligase complex, OsTIR1(F74G). The induced degradation is reversible 3 h after washout with fresh medium. 5-Ph-IAA shows rapid depletion of mAID-tagged nuclear and cytoplasmic proteins in proliferating and non-proliferating mammalian cells with no detectable basal degradation, lower cytotoxicity level, increased potency and faster degradation of the reporter in comparison with the conventional AID system. The AID2 system also works in budding yeast and fission yeast, and it can control protein expression in multiple mouse organs in vivo.
5-Ph-IAA-AM (Cat. No. 7893), an analog of 5-Ph-IAA, is also available.
To enquire about custom knock-in cell lines for the AID2 platform, please email TPD@bio-techne.com
Plasmid vectors for the AAV transfection and CRISPR-mediated knock-in of OsTIR1F74G and mAID tag are available from Addgene.
Technical Data for 5-Ph-IAA
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for 5-Ph-IAA
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for 5-Ph-IAA
The following data is based on the product molecular weight 251.29. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.98 mL||19.9 mL||39.79 mL|
|5 mM||0.8 mL||3.98 mL||7.96 mL|
|10 mM||0.4 mL||1.99 mL||3.98 mL|
|50 mM||0.08 mL||0.4 mL||0.8 mL|
References for 5-Ph-IAA
References are publications that support the biological activity of the product.
Yeung et al (2021) One-step multiplex toolkit for efficient generation of conditional gene silencing human cell lines. Mol.Biol.Cell 32 1320 PMID: 33979199
Yesbolatova et al (2020) The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat.Commun. 11 5701 PMID: 33177522
Zhang et al (2021) An improved auxin-inducible degron system for fission yeast. G3 Genes|Genomes|Genetics 12 PMID: 34849776
If you know of a relevant reference for 5-Ph-IAA, please let us know.
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Keywords: 5-Ph-IAA, 5-Ph-IAA supplier, Bump, Hole, auxin-inducible, degron, AID2, AID, TAG, Degrades, Degraders, degradation, target, fusion, protein, mAID, Degradation, Platform, Molecular, Glues, 7392, Tocris Bioscience
Citations for 5-Ph-IAA
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Literature in this Area
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Targeted Protein Degradation Research Product GuideUpdated
This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:
- Active Degraders
- TAG Degradation Platform
- Degrader Building Blocks
- Ubiquitin-Proteasome System Proteins
- Assays for Protein Degradation
Targeted Protein Degradation Poster
Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia