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Description: Selective and potent TAG Degrader for auxin-inducible degron 2 system; 5-Ph-IAA analog
Chemical Name: Acetoxymethyl 2-(5-phenyl-1H-indol-3-yl)acetate
Purity: ≥98% (HPLC)
Literature (3)

Biological Activity for 5-Ph-IAA-AM

5-Ph-IAA-AM is a potent and selective "Bump & Hole" TAG Degrader, for use within an auxin-inducible degron (AID2) system; it is an egg-permeant analog of 5-Ph-IAA (Cat. No. 7392). 5-Ph-IAA-AM induces selective degradation of a target protein tagged with a single-point mutant AtTIR1F79G F-box protein. 5-Ph-IAA-AM induces target protein degradation in C. elegans embryos. No developmental abnormality is observed when the embryos are treated with 50 μM 5-Ph-IAA-AM.

Technical Data for 5-Ph-IAA-AM

M. Wt 323.35
Formula C19H17NO4
Storage Store at -20°C
Purity ≥98% (HPLC)
PubChem ID 162641049
Smiles O=C(OCOC(C)=O)CC1=CNC2=C1C=C(C=C2)C3=CC=CC=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 5-Ph-IAA-AM

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 32.34 100
ethanol 3.23 10

Preparing Stock Solutions for 5-Ph-IAA-AM

The following data is based on the product molecular weight 323.35. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.09 mL 15.46 mL 30.93 mL
5 mM 0.62 mL 3.09 mL 6.19 mL
10 mM 0.31 mL 1.55 mL 3.09 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

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References for 5-Ph-IAA-AM

References are publications that support the biological activity of the product.

Negishi et al (2022) The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans. Genetics 220 iyab218 PMID: 34865044

If you know of a relevant reference for 5-Ph-IAA-AM, please let us know.

Keywords: 5-Ph-IAA-AM, 5-Ph-IAA-AM supplier, auxin-inducible, degron, AID2, AID, TAG, Degrades, Degraders, degradation, target, fusion, protein, mAID, 5-Ph-IAA, Degradation, Platform, 7893, Tocris Bioscience

Citations for 5-Ph-IAA-AM

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Literature in this Area

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Targeted Protein Degradation Research Product Guide

Targeted Protein Degradation Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:

  • Active Degraders
  • TAG Degradation Platform
  • Degrader Building Blocks
  • Ubiquitin-Proteasome System Proteins
  • Assays for Protein Degradation
Targeted Protein Degradation Poster

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia

Validating Targets for TPD Using dTAG Poster

Validating Targets for TPD Using dTAG Poster

The dTAG platform offers a generalizable strategy to degrade, in principle, any intracellular protein of interest (POI) and is a useful strategy for exploration and validation of targets. This poster presents a workflow solution for target validation, from custom TAG knock-in cell-lines for your POI, different dTAG Degraders to knockdown you POI, to automated assays for protein degradation using Simple WesternTM instruments. Data are also presented on the use of an antibody that recognizes the TAG domain (FKBP12F36V) for detection of degradation. Presented at TPD Europe, March 2022, London, UK.