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Description: Click-reactive fluorescent dye. Suitable for live cell imaging
Chemical Name: 3-Azido-7-hydroxy-2H-1-benzopyran-2-one
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for 3-Azido-7-hydroxycoumarin

Key information: 3-Azido-7-hydroxycoumarin is a water-soluble fluorogenic fluorescent dye. Supplied with an azide reactive handle for click chemistry. It emits intense fluorescence only after 'click' reaction when it forms triazoles at the site of alkyne modified biomolecules. Suitable for live-cell imaging.

Application: Fluorescence microscopy. Can be used to label DNA in proliferating cells following 5-Ethynyl-2'-deoxyuridine (Cat.No. 7207) incorporation. Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 404 nm and 477 nm, respectively, after click reaction.

Optical Data for 3-Azido-7-hydroxycoumarin

Emission Color Blue
λabs 404 nm
λem 477 nm
Application Fluorescence Microscopy

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Technical Data for 3-Azido-7-hydroxycoumarin

M. Wt 203.15
Formula C9H5N3O3
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 817638-68-9
PubChem ID 25127608
Smiles [N-]=[N+]=NC1=CC=2C=CC(O)=CC2OC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 3-Azido-7-hydroxycoumarin

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 20.32 100
DMF 20.32 100

Preparing Stock Solutions for 3-Azido-7-hydroxycoumarin

The following data is based on the product molecular weight 203.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.92 mL 24.61 mL 49.22 mL
5 mM 0.98 mL 4.92 mL 9.84 mL
10 mM 0.49 mL 2.46 mL 4.92 mL
50 mM 0.1 mL 0.49 mL 0.98 mL

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Product Datasheets for 3-Azido-7-hydroxycoumarin

References for 3-Azido-7-hydroxycoumarin

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Keywords: 3-Azido-7-hydroxycoumarin, 3-Azido-7-hydroxycoumarin supplier, 3azido7hydroxycoumarin, fluorescence, triazoles, Fluorescent, fluorogenic, dyes, dye, imaging, Click, Reactive, Click-Reactive, alkyne, Dyes, 7664, Tocris Bioscience

Citations for 3-Azido-7-hydroxycoumarin

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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