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Description: Click-reactive fluorescent dye
Chemical Name: 3-Azido-7-hydroxy-2H-1-benzopyran-2-one
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for 3-Azido-7-hydroxycoumarin

3-Azido-7-hydroxycoumarin is a water-soluble fluorogenic fluorescent dye. It emits intense fluorescence only after 'click' reaction when it forms triazoles at the site of alkyne modified biomolecules. Excitation and emission maxima are 404 nm and 477 nm respectively, after click reaction.

Optical Data for 3-Azido-7-hydroxycoumarin

Emission Color Blue
λabs 404 nm
λem 477 nm

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Technical Data for 3-Azido-7-hydroxycoumarin

M. Wt 203.15
Formula C9H5N3O3
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 817638-68-9
PubChem ID 25127608
Smiles [N-]=[N+]=NC1=CC=2C=CC(O)=CC2OC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 3-Azido-7-hydroxycoumarin

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 20.32 100
DMF 20.32 100

Preparing Stock Solutions for 3-Azido-7-hydroxycoumarin

The following data is based on the product molecular weight 203.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.92 mL 24.61 mL 49.22 mL
5 mM 0.98 mL 4.92 mL 9.84 mL
10 mM 0.49 mL 2.46 mL 4.92 mL
50 mM 0.1 mL 0.49 mL 0.98 mL

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Product Datasheets for 3-Azido-7-hydroxycoumarin

References for 3-Azido-7-hydroxycoumarin

References are publications that support the biological activity of the product.

Sivakumar et al (2004) A fluorogenic 1,3-dipolar cycloaddition reaction of 3-azidocoumarins and acetylenes. Org.Lett. 6 4603 PMID: 15548086

Li et al (2010) Fluorogenic "click" reaction for labeling and detection of DNA in proliferating cells. Biotechniques 49 525 PMID: 20615206

Liu et al (2020) CLICK-17, a DNA enzyme that harnesses ultra-low concentrations of either Cu+ or Cu2+ to catalyze the azide-alkyne 'click' reaction in water. Nucleic Acids Res 48 7356 PMID: 32520335

Bobrin et al (2021) Water-borne nanocoating for rapid inactivation of SARS-CoV-2 and other viruses. ACS Nano 15 14915 PMID: 34423970

Silini et al (2021) CM from intact hAM: an easily obtained product with relevant implications for translation in regenerative medicine. Stem Cell Res.Ther. 12 540 PMID: 34641958

If you know of a relevant reference for 3-Azido-7-hydroxycoumarin, please let us know.

Keywords: 3-Azido-7-hydroxycoumarin, 3-Azido-7-hydroxycoumarin supplier, 3azido7hydroxycoumarin, fluorescence, triazoles, Fluorescent, fluorogenic, dyes, dye, imaging, Click, Reactive, Click-Reactive, alkyne, Dyes, 7664, Tocris Bioscience

Citations for 3-Azido-7-hydroxycoumarin

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