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Submit Review3-Azido-7-hydroxycoumarin
Description: Click-reactive fluorescent dye
Chemical Name: 3-Azido-7-hydroxy-2H-1-benzopyran-2-one
Purity: ≥95% (HPLC)
Biological Activity for 3-Azido-7-hydroxycoumarin
3-Azido-7-hydroxycoumarin is a water-soluble fluorogenic fluorescent dye. It emits intense fluorescence only after 'click' reaction when it forms triazoles at the site of alkyne modified biomolecules. Excitation and emission maxima are 404 nm and 477 nm respectively, after click reaction.
Optical Data for 3-Azido-7-hydroxycoumarin
| Emission Color | Blue |
|---|---|
| λabs | 404 nm |
| λem | 477 nm |
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Technical Data for 3-Azido-7-hydroxycoumarin
| M. Wt | 203.15 |
| Formula | C9H5N3O3 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 817638-68-9 |
| PubChem ID | 25127608 |
| InChI Key | KKJNQKVCZCNJDI-UHFFFAOYSA-N |
| Smiles | [N-]=[N+]=NC1=CC=2C=CC(O)=CC2OC1=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for 3-Azido-7-hydroxycoumarin
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 20.32 | 100 | |
| DMF | 20.32 | 100 |
Preparing Stock Solutions for 3-Azido-7-hydroxycoumarin
The following data is based on the product molecular weight 203.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.92 mL | 24.61 mL | 49.22 mL |
| 5 mM | 0.98 mL | 4.92 mL | 9.84 mL |
| 10 mM | 0.49 mL | 2.46 mL | 4.92 mL |
| 50 mM | 0.1 mL | 0.49 mL | 0.98 mL |
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Product Datasheets for 3-Azido-7-hydroxycoumarin
References for 3-Azido-7-hydroxycoumarin
References are publications that support the biological activity of the product.
Sivakumar et al (2004) A fluorogenic 1,3-dipolar cycloaddition reaction of 3-azidocoumarins and acetylenes. Org.Lett. 6 4603 PMID: 15548086
Li et al (2010) Fluorogenic "click" reaction for labeling and detection of DNA in proliferating cells. Biotechniques 49 525 PMID: 20615206
Liu et al (2020) CLICK-17, a DNA enzyme that harnesses ultra-low concentrations of either Cu+ or Cu2+ to catalyze the azide-alkyne 'click' reaction in water. Nucleic Acids Res 48 7356 PMID: 32520335
Bobrin et al (2021) Water-borne nanocoating for rapid inactivation of SARS-CoV-2 and other viruses. ACS Nano 15 14915 PMID: 34423970
Silini et al (2021) CM from intact hAM: an easily obtained product with relevant implications for translation in regenerative medicine. Stem Cell Res.Ther. 12 540 PMID: 34641958
If you know of a relevant reference for 3-Azido-7-hydroxycoumarin, please let us know.
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Keywords: 3-Azido-7-hydroxycoumarin, 3-Azido-7-hydroxycoumarin supplier, 3azido7hydroxycoumarin, fluorescence, triazoles, Fluorescent, fluorogenic, dyes, dye, imaging, Click, Reactive, Click-Reactive, alkyne, Dyes, 7664, Tocris Bioscience
Citations for 3-Azido-7-hydroxycoumarin
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