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Description: Inhibits hepatitis C viral life cycle; also α-crystallin (cryAB) chaperone
Chemical Name: Cholest-5-ene-3β,25-diol
Purity: ≥95% (HPLC)
Citations (2)

Biological Activity for 25-Hydroxycholesterol

25-Hydroxycholesterol is an antiviral. Stimulates expression of miR-185 microRNA which inhibits the hepatitis C virus life cycle via inhibition of cholesterol biosynthesis and virus cell membrane fusion. Also α-crystallin (cryAB) chaperone (Kd = 10.1 μM). Blocks α-crystallin aggregation and partially reverses cataract formation by binding and stabilizing soluble forms of cryAB in vitro and in vivo.

Technical Data for 25-Hydroxycholesterol

M. Wt 402.65
Formula C27H46O2
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2140-46-7
PubChem ID 65094
Smiles O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCCC(C)(O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 25-Hydroxycholesterol

Solvent Max Conc. mg/mL Max Conc. mM
ethanol 8.05 20

Preparing Stock Solutions for 25-Hydroxycholesterol

The following data is based on the product molecular weight 402.65. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 12.42 mL 62.09 mL 124.18 mL
1 mM 2.48 mL 12.42 mL 24.84 mL
2 mM 1.24 mL 6.21 mL 12.42 mL
10 mM 0.25 mL 1.24 mL 2.48 mL

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Product Datasheets for 25-Hydroxycholesterol

Certificate of Analysis / Product Datasheet
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References for 25-Hydroxycholesterol

References are publications that support the biological activity of the product.

Singaravelu et al (2015) MicroRNAs regulate the immunometabolic response to viral infection in the liver. Nat.Chem.Biol. 11 988 PMID: 26479438

Makley et al (2015) Pharmacological chaperone for alpha-crystallin partially restores transparency in cataract models. Science 350 674 PMID: 26542570

If you know of a relevant reference for 25-Hydroxycholesterol, please let us know.

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Keywords: 25-Hydroxycholesterol, 25-Hydroxycholesterol supplier, Antiviral, alpha-crystallin, chaperone, a-crystallin, α-crystallin, microRNA, miR-185, hepatitis, C, virus, HCV, cholesterol, aggregation, cataract, cryAB, cryAA, amyloid, Hepatitis, Viruses, 5741, Tocris Bioscience

2 Citations for 25-Hydroxycholesterol

Citations are publications that use Tocris products. Selected citations for 25-Hydroxycholesterol include:

Judith et al (2022) Senolysis induced by 25-hydroxycholesterol targets CRYAB in multiple cell types. iScience 25 103848 PMID: 35198901

Keith S et al (2021) Listeria monocytogenes-infected human monocytic derived dendritic cells activate Vγ9Vδ2 T cells independently of HMBPP production. Sci Rep 11 16347 PMID: 34381163

Do you know of a great paper that uses 25-Hydroxycholesterol from Tocris? Please let us know.

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