These Stem Cell Protocol snapshots are intended to highlight how small molecules may be used in place of or in combination with growth factors and other proteins in cell culture, reprogramming and differentiation to generate Organoids. Using small molecules in organoid generation protocols offers several advantages:
- good temporal control: their effects are rapid and reversible
- they are cell permeable
- they are chemically synthesized, with low batch-to-batch variability
- have high purity
- animal-free production