Antibiotic; inhibits GlcNAc phosphotransferase (GPT). Blocks the formation of N-glycosidic linkages by inhibiting the first step in glycoprotein synthesis. Activity induces ER stress and causes G1 arrest; can be used to induce autophagy. Tunicamycin contains four main components as follows:
- Homolog A, n=8, C37H60N4O16, molecular weight = 816.90
- Homolog B, n=9, C38H62N4O16, molecular weight = 830.93
- Homolog C, n=10, C39H64N4O16, molecular weight = 844.95
- Homolog D, n=11, C40H66N4O16, molecular weight = 858.99
|Formula||C39H64N4O16 (tunicamycin C, n=10)|
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 844.95. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.18 mL||5.92 mL||11.84 mL|
|5 mM||0.24 mL||1.18 mL||2.37 mL|
|10 mM||0.12 mL||0.59 mL||1.18 mL|
|50 mM||0.02 mL||0.12 mL||0.24 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Duriez et al (2008) The hepatitis B virus precore protein is retrotransported from endoplasmic reticulum (ER) to cytosol through the ER-associated pathway. J.Biol.Chem. 283 32352 PMID: 18805786
Lauer et al (2009) Primary murine airway smooth muscle cells exposed to poly(I:C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix. J.Biol.Chem. 284 5299 PMID: 19088077
Ding et al (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 282 4702 PMID: 17135238
If you know of a relevant reference for Tunicamycin, please let us know.
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Keywords: antibiotics GlcNAc phosphotransferases GTP protein glycosylation inhibitors inhibits Other Transferases
1 Citation for Tunicamycin
Citations are publications that use Tocris products. Selected citations for Tunicamycin include:
Deldicque et al (2011) ER stress induces anabolic resistance in muscle cells through PKB-induced blockade of mTORC1. PLoS One 6 e20993 PMID: 21698202
Do you know of a great paper that uses Tunicamycin from Tocris? If so please let us know.
Literature in this Area
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