Tunicamycin

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Cat.No. 3516 - Tunicamycin | C39H64N4O16 (tunicamycin C, n=10) | CAS No. 11089-65-9
Description: Antibiotic; GlcNAc phosphotransferase inhibitor
Chemical Name: Tunicamycin from Streptomyces sp.
Datasheet
Citations (7)
Reviews (1)
Literature

Biological Activity

Antibiotic; inhibits GlcNAc phosphotransferase (GPT). Blocks the formation of N-glycosidic linkages by inhibiting the first step in glycoprotein synthesis. Activity induces ER stress and causes G1 arrest; can be used to induce autophagy. Tunicamycin contains four main components as follows:

  • Homolog A, n=8, C37H60N4O16, molecular weight = 816.90
  • Homolog B, n=9, C38H62N4O16, molecular weight = 830.93
  • Homolog C, n=10, C39H64N4O16, molecular weight = 844.95
  • Homolog D, n=11, C40H66N4O16, molecular weight = 858.99
The composition of this product will vary from batch to batch and can be found on the relevant certificate of analysis.

Technical Data

M. Wt 844.95
Formula C39H64N4O16 (tunicamycin C, n=10)
Storage Store at +4°C
CAS Number 11089-65-9
PubChem ID 90488851
InChI Key ZOCXUHJGZXXIGQ-SQXRCPDGSA-N
Smiles O=C(C=C3)NC(N3[C@@H]2O[C@@H]([C@@H](O)[C@H]2O)[C@H](O)C[C@@H]1[C@H](O)[C@H](O)[C@@H](NC(/C=C/CCCCCCCCCCC(C)C)=O)[C@H](OC4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4NC(C)=O)O1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 42.25 50

Preparing Stock Solutions

The following data is based on the product molecular weight 844.95. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.37 mL 11.84 mL 23.67 mL
2.5 mM 0.47 mL 2.37 mL 4.73 mL
5 mM 0.24 mL 1.18 mL 2.37 mL
25 mM 0.05 mL 0.24 mL 0.47 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Duriez et al (2008) The hepatitis B virus precore protein is retrotransported from endoplasmic reticulum (ER) to cytosol through the ER-associated pathway. J.Biol.Chem. 283 32352 PMID: 18805786

Lauer et al (2009) Primary murine airway smooth muscle cells exposed to poly(I:C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix. J.Biol.Chem. 284 5299 PMID: 19088077

Ding et al (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 282 4702 PMID: 17135238


If you know of a relevant reference for Tunicamycin, please let us know.

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Keywords: Tunicamycin, Tunicamycin supplier, antibiotics, GlcNAc, phosphotransferases, GTP, protein, glycosylation, inhibitors, inhibits, Other, Transferases, Antibiotics, Autophagy, 3516, Tocris Bioscience

7 Citations for Tunicamycin

Citations are publications that use Tocris products. Selected citations for Tunicamycin include:

Zhang et al (2019) A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem PMID: 30792309

Skrenkova et al (2018) N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors. Front Mol Neurosci 11 188 PMID: 29915530

Hossain et al (2018) Hyperactivation of nuclear receptor coactivators induces PERK-dependent cell death. Oncotarget 9 11707 PMID: 29545931

Greer et al (2018) ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget 9 18454 PMID: 29719618

Gupta et al (2016) NCOA3 coactivator is a transcriptional target of XBP1 and regulates PERK-eIF2α-ATF4 signalling in breast cancer. Oncogene 35 5860 PMID: 27109102

Bajikar et al (2017) Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell 43 418 PMID: 29161592

Deldicque et al (2011) ER stress induces anabolic resistance in muscle cells through PKB-induced blockade of mTORC1. PLoS One 6 e20993 PMID: 21698202


Do you know of a great paper that uses Tunicamycin from Tocris? Please let us know.

Reviews for Tunicamycin

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Tunicamycin Usage Information.
By Chao Li on 01/08/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Human Intestine Subepithelial myofibroblasts

It is working well and easily dissolved into DMSO. I used 2.5, 5 µg/ml of Tunicamycin with serum free culture medium to treat the cells for 8h, 16h, 24h, and 48h.


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