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Description: Antibiotic; GlcNAc phosphotransferase inhibitor
Chemical Name: Tunicamycin from Streptomyces sp.
Purity: ≥98% (HPLC)
Citations (26)
Reviews (4)

Biological Activity for Tunicamycin

Tunicamycin is an antibiotic; inhibits GlcNAc phosphotransferase (GPT). Blocks the formation of N-glycosidic linkages by inhibiting the first step in glycoprotein synthesis. Activity induces ER stress and causes G1 arrest; can be used to induce autophagy. Tunicamycin contains four main components as follows:

  • Homolog A, n=8, C37H60N4O16, molecular weight = 816.90
  • Homolog B, n=9, C38H62N4O16, molecular weight = 830.93
  • Homolog C, n=10, C39H64N4O16, molecular weight = 844.95
  • Homolog D, n=11, C40H66N4O16, molecular weight = 858.99
The composition of this product will vary from batch to batch and can be found on the relevant certificate of analysis.

Technical Data for Tunicamycin

M. Wt 844.95
Formula C39H64N4O16 (tunicamycin C, n=10)
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 11089-65-9
PubChem ID 90488851
Smiles O=C(C=C3)NC(N3[C@@H]2O[C@@H]([C@@H](O)[C@H]2O)[C@H](O)C[C@@H]1[C@H](O)[C@H](O)[C@@H](NC(/C=C/CCCCCCCCCCC(C)C)=O)[C@H](OC4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4NC(C)=O)O1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Tunicamycin

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 42.25 50

Preparing Stock Solutions for Tunicamycin

The following data is based on the product molecular weight 844.95. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.37 mL 11.84 mL 23.67 mL
2.5 mM 0.47 mL 2.37 mL 4.73 mL
5 mM 0.24 mL 1.18 mL 2.37 mL
25 mM 0.05 mL 0.24 mL 0.47 mL

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Product Datasheets for Tunicamycin

Certificate of Analysis / Product Datasheet
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References for Tunicamycin

References are publications that support the biological activity of the product.

Duriez et al (2008) The hepatitis B virus precore protein is retrotransported from endoplasmic reticulum (ER) to cytosol through the ER-associated pathway. J.Biol.Chem. 283 32352 PMID: 18805786

Lauer et al (2009) Primary murine airway smooth muscle cells exposed to poly(I:C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix. J.Biol.Chem. 284 5299 PMID: 19088077

Ding et al (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 282 4702 PMID: 17135238

If you know of a relevant reference for Tunicamycin, please let us know.

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Keywords: Tunicamycin, Tunicamycin supplier, antibiotics, GlcNAc, phosphotransferases, GTP, protein, glycosylation, inhibitors, inhibits, Other, Transferases, Antibiotics, Autophagy, 3516, Tocris Bioscience

26 Citations for Tunicamycin

Citations are publications that use Tocris products. Selected citations for Tunicamycin include:

Skrenkova et al (2018) N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors. Front Mol Neurosci 11 188 PMID: 29915530

Peter et al (2018) Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate. Mol Cell 71 629-636.e5 PMID: 30118681

Nathalie et al (2018) High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect. Elife 7 PMID: 30311906

Karolina M et al (2020) Probing natural variation of IRE1 expression and endoplasmic reticulum stress responses in Arabidopsis accessions. Sci Rep 10 19154 PMID: 33154475

Chao et al (2020) Endoplasmic Reticulum Stress in Subepithelial Myofibroblasts Increases the TGF-β1 Activity That Regulates Fibrosis in Crohn's Disease. Inflamm Bowel Dis 26 809-819 PMID: 32031621

Catarina et al (2020) Antagonistic relationship between the unfolded protein response and myocardin-driven transcription in smooth muscle. J Cell Physiol 235 7370-7382 PMID: 32039481

Nathalie et al (2020) MINPP1 prevents intracellular accumulation of the chelator inositol hexakisphosphate and is mutated in Pontocerebellar Hypoplasia. Nat Commun 11 6087 PMID: 33257696

Daniel R et al (2023) Staufen Impairs Autophagy in Neurodegeneration. Ann Neurol 93 398-416 PMID: 36151701

Mikhail et al (2023) Soluble Klotho protects against glomerular injury through regulation of ER stress response. Commun Biol 6 208 PMID: 36813870

Jochen H M et al (2023) Mannose metabolism inhibition sensitizes acute myeloid leukaemia cells to therapy by driving ferroptotic cell death. Nat Commun 14 2132 PMID: 37059720

Yatrik M et al (2023) Hepatic SEL1L-HRD1 ER-associated degradation regulates systemic iron homeostasis via ceruloplasmin. Proc Natl Acad Sci U S A 120 e2212644120 PMID: 36595688

François et al (2023) Maximizing protein production by keeping cells at optimal secretory stress levels using real-time control approaches. Nat Commun 14 3028 PMID: 37231013

Hossain et al (2018) Hyperactivation of nuclear receptor coactivators induces PERK-dependent cell death. Oncotarget 9 11707 PMID: 29545931

Greer et al (2018) ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget 9 18454 PMID: 29719618

Gupta et al (2016) NCOA3 coactivator is a transcriptional target of XBP1 and regulates PERK-eIF2α-ATF4 signalling in breast cancer. Oncogene 35 5860 PMID: 27109102

Bajikar et al (2017) Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell 43 418 PMID: 29161592

Zhang et al (2019) A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem PMID: 30792309

Deldicque et al (2011) ER stress induces anabolic resistance in muscle cells through PKB-induced blockade of mTORC1. PLoS One 6 e20993 PMID: 21698202

Jean-Christophe et al (2022) The Sec61 translocon is a therapeutic vulnerability in multiple myeloma. EMBO Mol Med 14 e14740 PMID: 35014767

Lu et al (2022) Systemic lipolysis promotes physiological fitness in Drosophila melanogaster. Aging (Albany NY) 14 6481-6506 PMID: 36044277

Parimal et al (2022) Integrated stress response restricts macrophage necroptosis. Life Sci Alliance 5 PMID: 34764207

Andrew V et al (2021) Targeting the IRE1α/XBP1 Endoplasmic Reticulum Stress Response Pathway in ARID1A-Mutant Ovarian Cancers. Cancer Res 81 5325-5335 PMID: 34548333

Troy et al (2021) A genome-wide CRISPR/Cas9 screen in acute myeloid leukemia cells identifies regulators of TAK-243 sensitivity. JCI Insight 6 PMID: 33476303

Ho-Chou et al (2021) Colchicine acts selectively in the liver to induce hepatokines that inhibit myeloid cell activation. Nat Metab 3 513-522 PMID: 33846641

Shemorry et al (2019) Caspase-mediated cleavage of IRE1 controls apoptotic cell commitment during endoplasmic reticulum stress. Elife 8 PMID: 31453810

Quincy A et al (2012) CHOP and caspase 3 induction underlie glioblastoma cell death in response to endoplasmic reticulum stress. Exp Ther Med 3 487-492 PMID: 22969916

Do you know of a great paper that uses Tunicamycin from Tocris? Please let us know.

Reviews for Tunicamycin

Average Rating: 4.8 (Based on 4 Reviews.)

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Good product.
By P Kumar on 04/19/2021
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: AC16

examine effect in AC16 cell line.

review image

Usage Information.
By Anonymous on 06/20/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: KMS11 or OPM2

5 μg/ml

I dissolved tunicamycin in DMSO. I treated cells for 16 hr.

PMID: 31453810 Reference
review image

Worked at once.
By Bibha Dahal on 01/08/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Human primary astrocytes

I dissolved tunicamycin in DMSO. I added tunicamycin at various concentrations (2uM, 4uM, 6uM, and 10uM) for 24 hours in human primary astrocytes and incubated at 37 C and 5% CO2. Next day, I collected lystates and ran a western blot. The first four lanes are tunicamycin added samples in increasing concentration and the last two lanes are without added. I looked for PERK activation using p-PERK antibody.

It took sometime to dissolve so you might want to warm once you add DMSO to tunicamycin in water bath.

review image

Tunicamycin Usage Information.
By Chao Li on 01/08/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Human Intestine Subepithelial myofibroblasts

It is working well and easily dissolved into DMSO. I used 2.5, 5 µg/ml of Tunicamycin with serum free culture medium to treat the cells for 8h, 16h, 24h, and 48h.