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Biological Activity for Tunicamycin
Tunicamycin is an antibiotic; inhibits GlcNAc phosphotransferase (GPT). Blocks the formation of N-glycosidic linkages by inhibiting the first step in glycoprotein synthesis. Activity induces ER stress and causes G1 arrest; can be used to induce autophagy. Tunicamycin contains four main components as follows:
- Homolog A, n=8, C37H60N4O16, molecular weight = 816.90
- Homolog B, n=9, C38H62N4O16, molecular weight = 830.93
- Homolog C, n=10, C39H64N4O16, molecular weight = 844.95
- Homolog D, n=11, C40H66N4O16, molecular weight = 858.99
Technical Data for Tunicamycin
|Formula||C39H64N4O16 (tunicamycin C, n=10)|
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Tunicamycin
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Tunicamycin
The following data is based on the product molecular weight 844.95. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.5 mM||2.37 mL||11.84 mL||23.67 mL|
|2.5 mM||0.47 mL||2.37 mL||4.73 mL|
|5 mM||0.24 mL||1.18 mL||2.37 mL|
|25 mM||0.05 mL||0.24 mL||0.47 mL|
References for Tunicamycin
References are publications that support the biological activity of the product.
Duriez et al (2008) The hepatitis B virus precore protein is retrotransported from endoplasmic reticulum (ER) to cytosol through the ER-associated pathway. J.Biol.Chem. 283 32352 PMID: 18805786
Lauer et al (2009) Primary murine airway smooth muscle cells exposed to poly(I:C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix. J.Biol.Chem. 284 5299 PMID: 19088077
Ding et al (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 282 4702 PMID: 17135238
If you know of a relevant reference for Tunicamycin, please let us know.
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Keywords: Tunicamycin, Tunicamycin supplier, antibiotics, GlcNAc, phosphotransferases, GTP, protein, glycosylation, inhibitors, inhibits, Other, Transferases, Antibiotics, Autophagy, 3516, Tocris Bioscience
8 Citations for Tunicamycin
Citations are publications that use Tocris products. Selected citations for Tunicamycin include:
Skrenkova et al (2018) N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors. Front Mol Neurosci 11 188 PMID: 29915530
Gupta et al (2016) NCOA3 coactivator is a transcriptional target of XBP1 and regulates PERK-eIF2α-ATF4 signalling in breast cancer. Oncogene 35 5860 PMID: 27109102
Shemorry et al (2019) Caspase-mediated cleavage of IRE1 controls apoptotic cell commitment during endoplasmic reticulum stress. Elife 8 PMID: 31453810
Deldicque et al (2011) ER stress induces anabolic resistance in muscle cells through PKB-induced blockade of mTORC1. PLoS One 6 e20993 PMID: 21698202
Zhang et al (2019) A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem PMID: 30792309
Bajikar et al (2017) Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell 43 418 PMID: 29161592
Hossain et al (2018) Hyperactivation of nuclear receptor coactivators induces PERK-dependent cell death. Oncotarget 9 11707 PMID: 29545931
Greer et al (2018) ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget 9 18454 PMID: 29719618
Do you know of a great paper that uses Tunicamycin from Tocris? Please let us know.
Reviews for Tunicamycin
Average Rating: 4.8 (Based on 4 Reviews.)
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examine effect in AC16 cell line.
I dissolved tunicamycin in DMSO. I treated cells for 16 hr.
I dissolved tunicamycin in DMSO. I added tunicamycin at various concentrations (2uM, 4uM, 6uM, and 10uM) for 24 hours in human primary astrocytes and incubated at 37 C and 5% CO2. Next day, I collected lystates and ran a western blot. The first four lanes are tunicamycin added samples in increasing concentration and the last two lanes are without added. I looked for PERK activation using p-PERK antibody.
It took sometime to dissolve so you might want to warm once you add DMSO to tunicamycin in water bath.
It is working well and easily dissolved into DMSO. I used 2.5, 5 µg/ml of Tunicamycin with serum free culture medium to treat the cells for 8h, 16h, 24h, and 48h.