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Biological Activity for Tunicamycin
Tunicamycin is an antibiotic; inhibits GlcNAc phosphotransferase (GPT). Blocks the formation of N-glycosidic linkages by inhibiting the first step in glycoprotein synthesis. Activity induces ER stress and causes G1 arrest; can be used to induce autophagy. Tunicamycin contains four main components as follows:
- Homolog A, n=8, C37H60N4O16, molecular weight = 816.90
- Homolog B, n=9, C38H62N4O16, molecular weight = 830.93
- Homolog C, n=10, C39H64N4O16, molecular weight = 844.95
- Homolog D, n=11, C40H66N4O16, molecular weight = 858.99
Technical Data for Tunicamycin
|Formula||C39H64N4O16 (tunicamycin C, n=10)|
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Tunicamycin
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Tunicamycin
The following data is based on the product molecular weight 844.95. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.5 mM||2.37 mL||11.84 mL||23.67 mL|
|2.5 mM||0.47 mL||2.37 mL||4.73 mL|
|5 mM||0.24 mL||1.18 mL||2.37 mL|
|25 mM||0.05 mL||0.24 mL||0.47 mL|
References for Tunicamycin
References are publications that support the biological activity of the product.
Duriez et al (2008) The hepatitis B virus precore protein is retrotransported from endoplasmic reticulum (ER) to cytosol through the ER-associated pathway. J.Biol.Chem. 283 32352 PMID: 18805786
Lauer et al (2009) Primary murine airway smooth muscle cells exposed to poly(I:C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix. J.Biol.Chem. 284 5299 PMID: 19088077
Ding et al (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 282 4702 PMID: 17135238
If you know of a relevant reference for Tunicamycin, please let us know.
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Keywords: Tunicamycin, Tunicamycin supplier, antibiotics, GlcNAc, phosphotransferases, GTP, protein, glycosylation, inhibitors, inhibits, Other, Transferases, Antibiotics, Autophagy, 3516, Tocris Bioscience
26 Citations for Tunicamycin
Citations are publications that use Tocris products. Selected citations for Tunicamycin include:
Skrenkova et al (2018) N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors. Front Mol Neurosci 11 188 PMID: 29915530
Gupta et al (2016) NCOA3 coactivator is a transcriptional target of XBP1 and regulates PERK-eIF2α-ATF4 signalling in breast cancer. Oncogene 35 5860 PMID: 27109102
Daniel R et al (2023) Staufen Impairs Autophagy in Neurodegeneration. Ann Neurol 93 398-416 PMID: 36151701
Mikhail et al (2023) Soluble Klotho protects against glomerular injury through regulation of ER stress response. Commun Biol 6 208 PMID: 36813870
Jochen H M et al (2023) Mannose metabolism inhibition sensitizes acute myeloid leukaemia cells to therapy by driving ferroptotic cell death. Nat Commun 14 2132 PMID: 37059720
Yatrik M et al (2023) Hepatic SEL1L-HRD1 ER-associated degradation regulates systemic iron homeostasis via ceruloplasmin. Proc Natl Acad Sci U S A 120 e2212644120 PMID: 36595688
François et al (2023) Maximizing protein production by keeping cells at optimal secretory stress levels using real-time control approaches. Nat Commun 14 3028 PMID: 37231013
Jean-Christophe et al (2022) The Sec61 translocon is a therapeutic vulnerability in multiple myeloma. EMBO Mol Med 14 e14740 PMID: 35014767
Lu et al (2022) Systemic lipolysis promotes physiological fitness in Drosophila melanogaster. Aging (Albany NY) 14 6481-6506 PMID: 36044277
Parimal et al (2022) Integrated stress response restricts macrophage necroptosis. Life Sci Alliance 5 PMID: 34764207
Hossain et al (2018) Hyperactivation of nuclear receptor coactivators induces PERK-dependent cell death. Oncotarget 9 11707 PMID: 29545931
Greer et al (2018) ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget 9 18454 PMID: 29719618
Quincy A et al (2012) CHOP and caspase 3 induction underlie glioblastoma cell death in response to endoplasmic reticulum stress. Exp Ther Med 3 487-492 PMID: 22969916
Andrew V et al (2021) Targeting the IRE1α/XBP1 Endoplasmic Reticulum Stress Response Pathway in ARID1A-Mutant Ovarian Cancers. Cancer Res 81 5325-5335 PMID: 34548333
Troy et al (2021) A genome-wide CRISPR/Cas9 screen in acute myeloid leukemia cells identifies regulators of TAK-243 sensitivity. JCI Insight 6 PMID: 33476303
Ho-Chou et al (2021) Colchicine acts selectively in the liver to induce hepatokines that inhibit myeloid cell activation. Nat Metab 3 513-522 PMID: 33846641
Nathalie et al (2020) MINPP1 prevents intracellular accumulation of the chelator inositol hexakisphosphate and is mutated in Pontocerebellar Hypoplasia. Nat Commun 11 6087 PMID: 33257696
Karolina M et al (2020) Probing natural variation of IRE1 expression and endoplasmic reticulum stress responses in Arabidopsis accessions. Sci Rep 10 19154 PMID: 33154475
Chao et al (2020) Endoplasmic Reticulum Stress in Subepithelial Myofibroblasts Increases the TGF-β1 Activity That Regulates Fibrosis in Crohn's Disease. Inflamm Bowel Dis 26 809-819 PMID: 32031621
Catarina et al (2020) Antagonistic relationship between the unfolded protein response and myocardin-driven transcription in smooth muscle. J Cell Physiol 235 7370-7382 PMID: 32039481
Deldicque et al (2011) ER stress induces anabolic resistance in muscle cells through PKB-induced blockade of mTORC1. PLoS One 6 e20993 PMID: 21698202
Peter et al (2018) Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate. Mol Cell 71 629-636.e5 PMID: 30118681
Nathalie et al (2018) High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect. Elife 7 PMID: 30311906
Bajikar et al (2017) Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell 43 418 PMID: 29161592
Shemorry et al (2019) Caspase-mediated cleavage of IRE1 controls apoptotic cell commitment during endoplasmic reticulum stress. Elife 8 PMID: 31453810
Zhang et al (2019) A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem PMID: 30792309
Do you know of a great paper that uses Tunicamycin from Tocris? Please let us know.
Reviews for Tunicamycin
Average Rating: 4.8 (Based on 4 Reviews.)
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examine effect in AC16 cell line.
I dissolved tunicamycin in DMSO. I treated cells for 16 hr.
I dissolved tunicamycin in DMSO. I added tunicamycin at various concentrations (2uM, 4uM, 6uM, and 10uM) for 24 hours in human primary astrocytes and incubated at 37 C and 5% CO2. Next day, I collected lystates and ran a western blot. The first four lanes are tunicamycin added samples in increasing concentration and the last two lanes are without added. I looked for PERK activation using p-PERK antibody.
It took sometime to dissolve so you might want to warm once you add DMSO to tunicamycin in water bath.
It is working well and easily dissolved into DMSO. I used 2.5, 5 µg/ml of Tunicamycin with serum free culture medium to treat the cells for 8h, 16h, 24h, and 48h.