trans-Ned 19

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Description: NAADP antagonist; inhibits Ca2+ release
Chemical Name: (1R,3S)-1-[3-[[4-(2-Fluorophenyl)piperazin-1-yl]methyl]-4-methoxyphenyl]-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid
Purity: ≥98% (HPLC)
Citations (6)

Biological Activity for trans-Ned 19

trans-Ned 19 is a nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist. Inhibits NAADP-mediated Ca2+ release (IC50 = 6 nM); also inhibits [32P]NAADP binding (IC50 = 0.4 nM). Fluorescently labels NAADP receptors in intact cells. Stereosiomer of cis-Ned 19 (Cat. No. 3953).

Optical Data for trans-Ned 19

λabs 368 nm
λem 425 nm
Closest Laser line 355 nm
Cell Permeable Yes
Application Confocal Microscopy

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Technical Data for trans-Ned 19

M. Wt 514.59
Formula C30H31FN4O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1354235-96-3
PubChem ID 1427628
Smiles FC1=CC=CC=C1N(CC6)CCN6CC2=C[C@@]([C@@H]3C4=C(C(C=CC=C5)=C5N4)C[C@@H](C(O)=O)N3)=CC=C2OC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for trans-Ned 19

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 51.46 100

Preparing Stock Solutions for trans-Ned 19

The following data is based on the product molecular weight 514.59. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.94 mL 9.72 mL 19.43 mL
5 mM 0.39 mL 1.94 mL 3.89 mL
10 mM 0.19 mL 0.97 mL 1.94 mL
50 mM 0.04 mL 0.19 mL 0.39 mL

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Product Datasheets for trans-Ned 19

Certificate of Analysis / Product Datasheet
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References for trans-Ned 19

References are publications that support the biological activity of the product.

Naylor et al (2009) Identification of a chemical probe for NAADP by virtual screening. Nat.Chem.Biol. 5 220 PMID: 19234453

Rosen et al (2009) Analogues of the nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist Ned-19 indicate two binding sites on the NAADP receptor. J.Biol.Chem. 284 34930 PMID: 19826006

Thai et al (2009) NAADP receptors mediate calcium signaling stimulated by endothelin-1 and NE in renal afferent arterioles. Am.J.Physiol.Renal Physiol. 297 F510 PMID: 19439521

If you know of a relevant reference for trans-Ned 19, please let us know.

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Keywords: trans-Ned 19, trans-Ned 19 supplier, NAAPD, nicotinic, acid, adenine, dinucleotide, phosphate, antagonists, calcium, signaling, signalling, inhibitors, inhibits, fluorescent, ned19, General, Calcium, Signaling, Agents, 3954, Tocris Bioscience

6 Citations for trans-Ned 19

Citations are publications that use Tocris products. Selected citations for trans-Ned 19 include:

Zhuo et al (2021) TPC2 promotes choroidal angiogenesis and inflammation in a mouse model of neovascular age-related macular degeneration. Life Sci Alliance 4 PMID: 34183443

Simon P et al (2021) Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis. Elife 10 PMID: 34677124

Favia et al (2016) NAADP-Dependent Ca(2+) Signaling Controls Melanoma Progression, Metastatic Dissemination and Neoangiogenesis. Toxicol Lett 6 18925 PMID: 26733361

Joe et al (2020) Cross-talk between CD38 and TTP is essential for resolution of inflammation during microbial sepsis. Cell Rep. 30 1063 PMID: 31995750

Do you know of a great paper that uses trans-Ned 19 from Tocris? Please let us know.

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