RF 9

Pricing Availability   Qty
Description: Selective NPFF1 and NPFF2 antagonist
Purity: ≥95% (HPLC)
Citations (3)

Biological Activity for RF 9

RF 9 is a selective NPFF receptor antagonist (Ki values are 58 and 75 nM at hNPFF1 and hNPFF2 receptors respectively). Displays selectivity over several related receptors including NPY Y1 GPR10, GPR54, GPR103 and the opioid receptors. Blocks NPFF-induced hypothermia and increases in atrial blood pressure and heart rate, and prevents opioid-induced tolerance associated with hyperalgesia in vivo.

Technical Data for RF 9

M. Wt 482.62
Formula C26H38N6O3
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 876310-60-0
PubChem ID 53320361
Smiles O=C(N[C@H]([C@](N[C@H](C(N)=O)CC4=CC=CC=C4)=O)CCCNC(N)=N)C1(CC(C3)C2)CC3CC2C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for RF 9

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 4.83 10

Preparing Stock Solutions for RF 9

The following data is based on the product molecular weight 482.62. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 20.72 mL 103.6 mL 207.2 mL
0.5 mM 4.14 mL 20.72 mL 41.44 mL
1 mM 2.07 mL 10.36 mL 20.72 mL
5 mM 0.41 mL 2.07 mL 4.14 mL

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Product Datasheets for RF 9

Certificate of Analysis / Product Datasheet
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References for RF 9

References are publications that support the biological activity of the product.

Wang et al (2008) Neuropeptide FF receptors antagonist, RF9, attenuates opioid-evoked hypothermia in mice. Peptides 29 1183 PMID: 18406009

Simonin et al (2006) RF9, a potent and selective neuropeptide FF receptor antagonist, prevents opioid-induced tolerance associated with hyperalgesia. Proc.Natl.Acad.Sci.USA 103 466

Fang et al (2008) Inhibition of neuropeptide FF (NPFF)-induced hypothermia and anti-morphine analgesia by RF9, a new selective NPFF receptors antagonist. Regul.Peptides 147 45

If you know of a relevant reference for RF 9, please let us know.

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Keywords: RF 9, RF 9 supplier, Selective, NPFF1, NPFF2, antagonists, npffr1, npffr2, Neuropeptide, FF, AF, Receptors, FF/AF, RF9, neuropeptides, 3672, Tocris Bioscience

3 Citations for RF 9

Citations are publications that use Tocris products. Selected citations for RF 9 include:

Jian et al (2023) RFRP-3 Influences Apoptosis and Steroidogenesis of Yak Cumulus Cells and Compromises Oocyte Meiotic Maturation and Subsequent Developmental Competence. Int J Mol Sci 24 PMID: 37108163

Alsaqati et al (2014) Novel vasocontractile role of the P2Y14 receptor: characterization of its signalling in porcine isolated pancreatic arteries. Br J Pharmacol 171 701 PMID: 24138077

Lee et al (2017) Genetic and neuronal regulation of sleep by neuropeptide VF. Elife 6 PMID: 29106375

Do you know of a great paper that uses RF 9 from Tocris? Please let us know.

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